Gene description for HACD3
Gene name 3-hydroxyacyl-CoA dehydratase 3
Gene symbol HACD3
Other names/aliases B-IND1
BIND1
HSPC121
PTPLAD1
Species Homo sapiens
 Database cross references - HACD3
ExoCarta ExoCarta_51495
Entrez Gene 51495
HGNC 24175
MIM 615940
UniProt Q9P035  
 HACD3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HACD3
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IDA
    GTPase activator activity GO:0005096 TAS
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IDA
Biological Process
    Rho protein signal transduction GO:0007266 IEA
    fatty acid elongation GO:0030497 IDA
    positive regulation of GTPase activity GO:0043547 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of viral genome replication GO:0045070 IMP
    very long-chain fatty acid biosynthetic process GO:0042761 IDA
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IEA
    Rac protein signal transduction GO:0016601 NAS
    positive regulation by virus of viral protein levels in host cell GO:0046726 IMP
    activation of JUN kinase activity GO:0007257 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified HACD3 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HACD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRF2  
Affinity Capture-MS Homo sapiens
2 RAC1 5879
Invivo Homo sapiens
3 PELO 53918
Affinity Capture-MS Homo sapiens
4 C20orf24  
Affinity Capture-MS Homo sapiens
5 SERINC3 10955
Affinity Capture-MS Homo sapiens
6 NEK6 10783
Affinity Capture-MS Homo sapiens
7 TSPAN17  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HACD3 is involved
PathwayEvidenceSource
Synthesis of very long-chain fatty acyl-CoAs TAS Reactome





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