Gene description for HACD3
Gene name
3-hydroxyacyl-CoA dehydratase 3
Gene symbol
HACD3
Other names/aliases
B-IND1 BIND1 HSPC121 PTPLAD1
Species
Homo sapiens
Database cross references - HACD3
ExoCarta
ExoCarta_51495
Entrez Gene
51495
HGNC
24175
MIM
615940
UniProt
Q9P035
HACD3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells
23333927
Ovarian cancer cells
23333927
Squamous carcinoma cells
20124223
Gene ontology annotations for HACD3
Experiment description of studies that identified HACD3 in exosomes
1
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
2
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
191
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for HACD3
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
BRF2
Affinity Capture-MS
Homo sapiens
2
RAC1
5879
Invivo
Homo sapiens
3
PELO
53918
Affinity Capture-MS
Homo sapiens
4
C20orf24
Affinity Capture-MS
Homo sapiens
5
SERINC3
10955
Affinity Capture-MS
Homo sapiens
6
NEK6
10783
Affinity Capture-MS
Homo sapiens
7
TSPAN17
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which HACD3 is involved