Gene description for SEC63
Gene name SEC63 homolog (S. cerevisiae)
Gene symbol SEC63
Other names/aliases DNAJC23
ERdj2
PRO2507
SEC63L
Species Homo sapiens
 Database cross references - SEC63
ExoCarta ExoCarta_11231
Vesiclepedia VP_11231
Entrez Gene 11231
HGNC 21082
MIM 608648
UniProt Q9UGP8  
 SEC63 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SEC63
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 IBA
    signaling receptor activity GO:0038023 TAS
Biological Process
    liver development GO:0001889 IEA
    protein targeting to membrane GO:0006612 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 IBA
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 IMP
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 IBA
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 IMP
    post-translational protein targeting to membrane, translocation GO:0031204 IMP
    nitrogen cycle metabolic process GO:0071941 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    membrane GO:0016020 IDA
    Sec62/Sec63 complex GO:0031207 IBA
 Experiment description of studies that identified SEC63 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SEC63
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 CHMP2A 27243
Cross-Linking-MS (XL-MS) Homo sapiens
3 SCFD1 23256
Proximity Label-MS Homo sapiens
4 HACD3 51495
Affinity Capture-MS Homo sapiens
5 ITGB1 3688
Proximity Label-MS Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 SENP3 26168
Affinity Capture-MS Homo sapiens
8 ANKLE2 23141
Proximity Label-MS Homo sapiens
9 ATP13A1 57130
Proximity Label-MS Homo sapiens
10 STT3A 3703
Affinity Capture-MS Homo sapiens
11 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
12 METTL7A 25840
Proximity Label-MS Homo sapiens
13 MOSPD2 158747
Proximity Label-MS Homo sapiens
14 SRPRB 58477
Proximity Label-MS Homo sapiens
15 SUCO  
Proximity Label-MS Homo sapiens
16 FA2H  
Affinity Capture-MS Homo sapiens
17 ABCC1 4363
Co-fractionation Homo sapiens
18 JPH1 56704
Proximity Label-MS Homo sapiens
19 SRPR 6734
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
20 STIM2 57620
Proximity Label-MS Homo sapiens
21 SPNS1 83985
Affinity Capture-MS Homo sapiens
22 HSD17B11 51170
Proximity Label-MS Homo sapiens
23 DNAJC25 548645
Proximity Label-MS Homo sapiens
24 SPA17  
Affinity Capture-MS Homo sapiens
25 PTPN1 5770
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
26 STX4 6810
Proximity Label-MS Homo sapiens
27 RTN4 57142
Proximity Label-MS Homo sapiens
28 SLC12A2 6558
Proximity Label-MS Homo sapiens
29 PMF1  
Affinity Capture-MS Homo sapiens
30 TMPO 7112
Proximity Label-MS Homo sapiens
31 ITPR3 3710
Proximity Label-MS Homo sapiens
32 KIAA1429 25962
Affinity Capture-MS Homo sapiens
33 VANGL2  
Proximity Label-MS Homo sapiens
34 RPL4 6124
Affinity Capture-MS Homo sapiens
35 TEX264 51368
Proximity Label-MS Homo sapiens
36 B3GAT1  
Proximity Label-MS Homo sapiens
37 PRR11  
Proximity Label-MS Homo sapiens
38 CACNG3  
Affinity Capture-MS Homo sapiens
39 WDR41  
Proximity Label-MS Homo sapiens
40 RINT1 60561
Proximity Label-MS Homo sapiens
41 FNDC3A 22862
Proximity Label-MS Homo sapiens
42 TRAM1 23471
Affinity Capture-MS Homo sapiens
43 RAB3B 5865
Proximity Label-MS Homo sapiens
44 TRDN 10345
Affinity Capture-MS Homo sapiens
45 RPL18 6141
Affinity Capture-MS Homo sapiens
46 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 GPRC5B 51704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SSR1 6745
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
49 GPBAR1  
Affinity Capture-MS Homo sapiens
50 RPS5 6193
Co-fractionation Homo sapiens
51 DDOST 1650
Co-fractionation Homo sapiens
52 SRP14 6727
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
53 ATP5O 539
Proximity Label-MS Homo sapiens
54 GPR183  
Affinity Capture-MS Homo sapiens
55 C3orf52  
Affinity Capture-MS Homo sapiens
56 VAPB 9217
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
57 DNAJC16  
Proximity Label-MS Homo sapiens
58 NFXL1 152518
Proximity Label-MS Homo sapiens
59 ABCD3 5825
Affinity Capture-MS Homo sapiens
60 SLC25A46 91137
Proximity Label-MS Homo sapiens
61 CCDC115 84317
Proximity Label-MS Homo sapiens
62 MXRA7 439921
Proximity Label-MS Homo sapiens
63 LSG1  
Proximity Label-MS Homo sapiens
64 MTDH 92140
Proximity Label-MS Homo sapiens
65 ATP5H 10476
Proximity Label-MS Homo sapiens
66 MECP2 4204
Affinity Capture-MS Homo sapiens
67 RAB35 11021
Proximity Label-MS Homo sapiens
68 ESYT2 57488
Affinity Capture-MS Homo sapiens
69 MMGT1 93380
Proximity Label-MS Homo sapiens
70 ATP6AP1 537
Proximity Label-MS Homo sapiens
71 CLDND1 56650
Affinity Capture-MS Homo sapiens
72 FAS 355
Proximity Label-MS Homo sapiens
73 CAMLG 819
Proximity Label-MS Homo sapiens
74 LMAN1 3998
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 KIAA1715 80856
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
76 DNAJC14  
Proximity Label-MS Homo sapiens
77 LRRC59 55379
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
78 AUP1 550
Proximity Label-MS Homo sapiens
79 KCNK1  
Affinity Capture-MS Homo sapiens
80 PIGH  
Affinity Capture-MS Homo sapiens
81 EIF2AK3  
Proximity Label-MS Homo sapiens
82 PANX1 24145
Proximity Label-MS Homo sapiens
83 LPAR2 9170
Affinity Capture-MS Homo sapiens
84 EMC10 284361
Proximity Label-MS Homo sapiens
85 SEC61A2 55176
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 SMPD4 55627
Proximity Label-MS Homo sapiens
87 RABL3 285282
Proximity Label-MS Homo sapiens
88 EMD 2010
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
89 STEAP3 55240
Affinity Capture-MS Homo sapiens
90 GAL  
Affinity Capture-MS Homo sapiens
91 TMX1 81542
Proximity Label-MS Homo sapiens
92 RB1CC1 9821
Affinity Capture-MS Homo sapiens
93 TBL2 26608
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 OSBPL8 114882
Proximity Label-MS Homo sapiens
95 CXADR 1525
Proximity Label-MS Homo sapiens
96 RPS10 6204
Affinity Capture-MS Homo sapiens
97 C17orf49 124944
Affinity Capture-MS Homo sapiens
98 G3bp1  
Two-hybrid Mus musculus
99 SEC22B 9554
Proximity Label-MS Homo sapiens
100 ADCY9 115
Proximity Label-MS Homo sapiens
101 KPNA4 3840
Co-fractionation Homo sapiens
102 CACNG4  
Affinity Capture-MS Homo sapiens
103 PGRMC2 10424
Proximity Label-MS Homo sapiens
104 CDKAL1  
Proximity Label-MS Homo sapiens
105 ATP2A2 488
Affinity Capture-MS Homo sapiens
106 CLCC1 23155
Proximity Label-MS Homo sapiens
107 RFX3 5991
Affinity Capture-MS Homo sapiens
108 NTRK1 4914
Affinity Capture-MS Homo sapiens
109 NCR1  
Affinity Capture-MS Homo sapiens
110 CDKN3 1033
Affinity Capture-MS Homo sapiens
111 ACADM 34
Co-fractionation Homo sapiens
112 C19orf25  
Proximity Label-MS Homo sapiens
113 RCC1 1104
Proximity Label-MS Homo sapiens
114 P4HB 5034
Proximity Label-MS Homo sapiens
115 RPA4  
Proximity Label-MS Homo sapiens
116 DHFRL1  
Proximity Label-MS Homo sapiens
117 AKAP7 9465
Affinity Capture-MS Homo sapiens
118 ACBD5 91452
Proximity Label-MS Homo sapiens
119 STX5 6811
Proximity Label-MS Homo sapiens
120 DDX54 79039
Proximity Label-MS Homo sapiens
121 TECR 9524
Affinity Capture-MS Homo sapiens
122 MARCKSL1 65108
Proximity Label-MS Homo sapiens
123 DNAJC30  
Proximity Label-MS Homo sapiens
124 TMEM131  
Proximity Label-MS Homo sapiens
125 SND1 27044
Proximity Label-MS Homo sapiens
126 ELOVL5 60481
Proximity Label-MS Homo sapiens
127 PNMA6A  
Affinity Capture-MS Homo sapiens
128 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
129 DNAJB11 51726
Proximity Label-MS Homo sapiens
130 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 RMDN3 55177
Proximity Label-MS Homo sapiens
132 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
133 REEP5 7905
Proximity Label-MS Homo sapiens
134 PKMYT1  
Proximity Label-MS Homo sapiens
135 HSPA5 3309
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
136 SMCR8 140775
Proximity Label-MS Homo sapiens
137 TMEM199  
Proximity Label-MS Homo sapiens
138 PREB 10113
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
139 EMC3 55831
Proximity Label-MS Homo sapiens
140 RPN2 6185
Proximity Label-MS Homo sapiens
141 SCD5  
Proximity Label-MS Homo sapiens
142 EMC8 10328
Proximity Label-MS Homo sapiens
143 CALR3  
Proximity Label-MS Homo sapiens
144 LMNB1 4001
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
145 ATP5F1 515
Affinity Capture-MS Homo sapiens
146 TRPM7 54822
Proximity Label-MS Homo sapiens
147 HMOX2 3163
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
148 EEF2 1938
Cross-Linking-MS (XL-MS) Homo sapiens
149 LMNB2 84823
Proximity Label-MS Homo sapiens
150 TAPT1 202018
Proximity Label-MS Homo sapiens
151 CISD2 493856
Proximity Label-MS Homo sapiens
152 BCAP31 10134
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
153 HSD3B7 80270
Proximity Label-MS Homo sapiens
154 ATP6AP2 10159
Proximity Label-MS Homo sapiens
155 NR3C1 2908
Proximity Label-MS Homo sapiens
156 STIM1 6786
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
157 NIPAL1  
Affinity Capture-MS Homo sapiens
158 FKBP8 23770
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
159 ARF6 382
Proximity Label-MS Homo sapiens
160 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 GPM6B 2824
Affinity Capture-MS Homo sapiens
162 SPCS3 60559
Proximity Label-MS Homo sapiens
163 GRAMD1A  
Proximity Label-MS Homo sapiens
164 EIF4H 7458
Affinity Capture-MS Homo sapiens
165 SOAT1 6646
Proximity Label-MS Homo sapiens
166 ACSL3 2181
Proximity Label-MS Homo sapiens
167 C3orf18  
Affinity Capture-MS Homo sapiens
168 RPA3 6119
Proximity Label-MS Homo sapiens
169 ATP2A1 487
Proximity Label-MS Homo sapiens
170 EMC2 9694
Proximity Label-MS Homo sapiens
171 CACFD1  
Affinity Capture-MS Homo sapiens
172 RAB9A 9367
Proximity Label-MS Homo sapiens
173 GSTK1 373156
Affinity Capture-MS Homo sapiens
174 STT3B 201595
Proximity Label-MS Homo sapiens
175 NCLN 56926
Affinity Capture-MS Homo sapiens
176 VRK2 7444
Proximity Label-MS Homo sapiens
177 YKT6 10652
Proximity Label-MS Homo sapiens
178 DNAJC3 5611
Proximity Label-MS Homo sapiens
179 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 Bub1b 12236
Affinity Capture-MS Mus musculus
181 DDRGK1 65992
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
182 KTN1 3895
Proximity Label-MS Homo sapiens
183 CYP2C9  
Proximity Label-MS Homo sapiens
184 ARGLU1  
Affinity Capture-MS Homo sapiens
185 FSCN1 6624
Proximity Label-MS Homo sapiens
186 LEMD3  
Proximity Label-MS Homo sapiens
187 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
188 IRF1  
Affinity Capture-MS Homo sapiens
189 AKAP1 8165
Proximity Label-MS Homo sapiens
190 TOMM20 9804
Affinity Capture-MS Homo sapiens
191 HUWE1 10075
Affinity Capture-MS Homo sapiens
192 TMEM231 79583
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
193 UBE2J1 51465
Proximity Label-MS Homo sapiens
194 SLC16A1 6566
Affinity Capture-MS Homo sapiens
195 TOR1AIP2 163590
Proximity Label-MS Homo sapiens
196 MBOAT7 79143
Proximity Label-MS Homo sapiens
197 LBR 3930
Proximity Label-MS Homo sapiens
198 POLE4  
Affinity Capture-MS Homo sapiens
199 SUN1 23353
Proximity Label-MS Homo sapiens
200 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
201 FASN 2194
Negative Genetic Homo sapiens
202 ALDH3A2 224
Proximity Label-MS Homo sapiens
203 STK16 8576
Affinity Capture-MS Homo sapiens
204 TRIM13  
Proximity Label-MS Homo sapiens
205 RRBP1 6238
Proximity Label-MS Homo sapiens
206 TMEM57  
Proximity Label-MS Homo sapiens
207 UBXN4 23190
Proximity Label-MS Homo sapiens
208 RAB3GAP2 25782
Proximity Label-MS Homo sapiens
209 TP53 7157
Synthetic Growth Defect Homo sapiens
210 AGPAT9  
Proximity Label-MS Homo sapiens
211 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
212 VANGL1 81839
Proximity Label-MS Homo sapiens
213 TMEM214 54867
Proximity Label-MS Homo sapiens
214 PDIA4 9601
Proximity Label-MS Homo sapiens
215 RAB3GAP1 22930
Proximity Label-MS Homo sapiens
216 CALU 813
Proximity Label-MS Homo sapiens
217 EMC1 23065
Proximity Label-MS Homo sapiens
218 EMC7 56851