Gene description for OSBPL8
Gene name oxysterol binding protein-like 8
Gene symbol OSBPL8
Other names/aliases MST120
MSTP120
ORP8
OSBP10
Species Homo sapiens
 Database cross references - OSBPL8
ExoCarta ExoCarta_114882
Vesiclepedia VP_114882
Entrez Gene 114882
HGNC 16396
MIM 606736
UniProt Q9BZF1  
 OSBPL8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for OSBPL8
Molecular Function
    phosphatidylserine binding GO:0001786 IDA
    protein binding GO:0005515 IPI
    phospholipid transporter activity GO:0005548 TAS
    cholesterol binding GO:0015485 IBA
    cholesterol binding GO:0015485 IDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    phosphatidylserine transfer activity GO:0140343 IDA
Biological Process
    negative regulation of sequestering of triglyceride GO:0010891 IDA
    phospholipid transport GO:0015914 IDA
    negative regulation of cell migration GO:0030336 IEA
    phosphatidylserine acyl-chain remodeling GO:0036150 TAS
    fat cell differentiation GO:0045444 IDA
    positive regulation of D-glucose import GO:0046326 ISS
    positive regulation of insulin receptor signaling pathway GO:0046628 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    protein localization to nuclear pore GO:0090204 IEA
    intermembrane lipid transfer GO:0120009 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    nuclear membrane GO:0031965 IEA
    cortical endoplasmic reticulum GO:0032541 IDA
 Experiment description of studies that identified OSBPL8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for OSBPL8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OR2M7  
Affinity Capture-MS Homo sapiens
2 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 TMBIM6 7009
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 FGD1  
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
9 COPB2 9276
Affinity Capture-MS Homo sapiens
10 GJD3  
Proximity Label-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 DNAJC25 548645
Proximity Label-MS Homo sapiens
14 COPE 11316
Affinity Capture-MS Homo sapiens
15 GPR114  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PTPN1 5770
Proximity Label-MS Homo sapiens
17 STX4 6810
Proximity Label-MS Homo sapiens
18 RTN4 57142
Affinity Capture-MS Homo sapiens
19 CAPZB 832
Affinity Capture-MS Homo sapiens
20 TEX264 51368
Affinity Capture-MS Homo sapiens
21 B3GAT1  
Proximity Label-MS Homo sapiens
22 INSRR 3645
Affinity Capture-MS Homo sapiens
23 GLG1 2734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 OR6T1  
Affinity Capture-MS Homo sapiens
25 P2RY1  
Affinity Capture-MS Homo sapiens
26 CXADR 1525
Proximity Label-MS Homo sapiens
27 FAM8A1 51439
Affinity Capture-MS Homo sapiens
28 RAB3B 5865
Proximity Label-MS Homo sapiens
29 UBXN6 80700
Affinity Capture-MS Homo sapiens
30 GLT8D2 83468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MDC1  
Affinity Capture-MS Homo sapiens
32 ZNF460  
Affinity Capture-MS Homo sapiens
33 SSR1 6745
Proximity Label-MS Homo sapiens
34 RER1 11079
Affinity Capture-MS Homo sapiens
35 PIGH  
Affinity Capture-MS Homo sapiens
36 CLEC14A  
Affinity Capture-MS Homo sapiens
37 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 C3orf52  
Affinity Capture-MS Homo sapiens
39 VAPB 9217
Affinity Capture-MS Homo sapiens
40 DNAJC16  
Proximity Label-MS Homo sapiens
41 DGCR2 9993
Affinity Capture-MS Homo sapiens
42 ATG16L1 55054
Affinity Capture-MS Homo sapiens
43 IL17RA 23765
Affinity Capture-MS Homo sapiens
44 RAB35 11021
Proximity Label-MS Homo sapiens
45 ESYT2 57488
Affinity Capture-MS Homo sapiens
46 CSNK2B 1460
Affinity Capture-MS Homo sapiens
47 OTOP1  
Affinity Capture-MS Homo sapiens
48 MARCKS 4082
Proximity Label-MS Homo sapiens
49 LAMTOR1 55004
Proximity Label-MS Homo sapiens
50 LMAN1 3998
Proximity Label-MS Homo sapiens
51 NUP155 9631
Proximity Label-MS Homo sapiens
52 KIAA1715 80856
Proximity Label-MS Homo sapiens
53 DNAJC14  
Proximity Label-MS Homo sapiens
54 RAB5A 5868
Proximity Label-MS Homo sapiens
55 FAM96A  
Affinity Capture-MS Homo sapiens
56 PANX1 24145
Proximity Label-MS Homo sapiens
57 EMD 2010
Proximity Label-MS Homo sapiens
58 C9orf78 51759
Affinity Capture-MS Homo sapiens
59 RB1CC1 9821
Affinity Capture-MS Homo sapiens
60 PARK2  
Affinity Capture-MS Homo sapiens
61 AMFR 267
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SLC2A8 29988
Affinity Capture-MS Homo sapiens
63 AVPR1B  
Affinity Capture-MS Homo sapiens
64 ZDHHC12  
Affinity Capture-MS Homo sapiens
65 RAB4A 5867
Proximity Label-MS Homo sapiens
66 DNAJC5 80331
Proximity Label-MS Homo sapiens
67 CHMP4B 128866
Affinity Capture-MS Homo sapiens
68 NTRK1 4914
Affinity Capture-MS Homo sapiens
69 STX6 10228
Proximity Label-MS Homo sapiens
70 LGALS1 3956
Affinity Capture-MS Homo sapiens
71 DHFRL1  
Proximity Label-MS Homo sapiens
72 EIF2B5 8893
Affinity Capture-MS Homo sapiens
73 RXFP2  
Affinity Capture-MS Homo sapiens
74 ELOVL5 60481
Proximity Label-MS Homo sapiens
75 NMUR2  
Affinity Capture-MS Homo sapiens
76 CT45A5  
Affinity Capture-MS Homo sapiens
77 PTGIR  
Affinity Capture-MS Homo sapiens
78 REEP5 7905
Proximity Label-MS Homo sapiens
79 CYP26B1  
Affinity Capture-MS Homo sapiens
80 SEC63 11231
Proximity Label-MS Homo sapiens
81 CNGA4  
Affinity Capture-MS Homo sapiens
82 HADHA 3030
Affinity Capture-MS Homo sapiens
83 RPN2 6185
Proximity Label-MS Homo sapiens
84 ACTBL2 345651
Affinity Capture-MS Homo sapiens
85 RAB7A 7879
Proximity Label-MS Homo sapiens
86 SMEK2  
Co-fractionation Homo sapiens
87 LRRC59 55379
Proximity Label-MS Homo sapiens
88 EGLN3  
Affinity Capture-MS Homo sapiens
89 SIRT7  
Affinity Capture-MS Homo sapiens
90 LAMP1 3916
Proximity Label-MS Homo sapiens
91 TMEM9B 56674
Affinity Capture-MS Homo sapiens
92 RSU1 6251
Cross-Linking-MS (XL-MS) Homo sapiens
93 GOLT1B 51026
Affinity Capture-MS Homo sapiens
94 BCAP31 10134
Proximity Label-MS Homo sapiens
95 HSD3B7 80270
Proximity Label-MS Homo sapiens
96 CUL3 8452
Affinity Capture-MS Homo sapiens
97 YWHAZ 7534
Affinity Capture-MS Homo sapiens
98 STIM1 6786
Proximity Label-MS Homo sapiens
99 CHRNA7  
Affinity Capture-MS Homo sapiens
100 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
101 FKBP8 23770
Proximity Label-MS Homo sapiens
102 NAGLU 4669
Affinity Capture-MS Homo sapiens
103 ARF6 382
Proximity Label-MS Homo sapiens
104 METTL21B  
Affinity Capture-MS Homo sapiens
105 FAM187B  
Affinity Capture-MS Homo sapiens
106 RIPK4  
Affinity Capture-MS Homo sapiens
107 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 RPN1 6184
Proximity Label-MS Homo sapiens
109 FABP6  
Affinity Capture-MS Homo sapiens
110 PCDHGB2  
Affinity Capture-MS Homo sapiens
111 PSPC1 55269
Affinity Capture-MS Homo sapiens
112 HECW2  
Affinity Capture-MS Homo sapiens
113 ATP2A1 487
Proximity Label-MS Homo sapiens
114 FBXO2 26232
Affinity Capture-MS Homo sapiens
115 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
116 RAB9A 9367
Proximity Label-MS Homo sapiens
117 OTULIN 90268
Affinity Capture-MS Homo sapiens
118 DDRGK1 65992
Affinity Capture-MS Homo sapiens
119 RHOB 388
Proximity Label-MS Homo sapiens
120 TAS1R2  
Affinity Capture-MS Homo sapiens
121 AVPR2  
Affinity Capture-MS Homo sapiens
122 EMC6 83460
Affinity Capture-MS Homo sapiens
123 MRPL42  
Affinity Capture-MS Homo sapiens
124 HLA-G 3135
Affinity Capture-MS Homo sapiens
125 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
126 TMED10 10972
Affinity Capture-MS Homo sapiens
127 TMEM9 252839
Affinity Capture-MS Homo sapiens
128 KIF14 9928
Affinity Capture-MS Homo sapiens
129 DNAJC19 131118
Affinity Capture-MS Homo sapiens
130 PGRMC1 10857
Affinity Capture-MS Homo sapiens
131 ERLIN1 10613
Affinity Capture-MS Homo sapiens
132 GPR180  
Affinity Capture-MS Homo sapiens
133 ZNF746  
Affinity Capture-MS Homo sapiens
134 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 EMC4 51234
Affinity Capture-MS Homo sapiens
136 SMURF1 57154
Affinity Capture-MS Homo sapiens
137 SQSTM1 8878
Affinity Capture-MS Homo sapiens
138 ERLIN2 11160
Affinity Capture-MS Homo sapiens
139 SNAP25 6616
PCA Homo sapiens
140 OR1M1  
Affinity Capture-MS Homo sapiens
141 C11orf52 91894
Proximity Label-MS Homo sapiens
142 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 TMEM171  
Affinity Capture-MS Homo sapiens
144 IFNLR1  
Affinity Capture-MS Homo sapiens
145 UFL1 23376
Affinity Capture-MS Homo sapiens
146 PXMP2  
Proximity Label-MS Homo sapiens
147 ERGIC2 51290
Proximity Label-MS Homo sapiens
148 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 SEC62 7095
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 PTPRCAP 5790
Affinity Capture-MS Homo sapiens
154 TMED3 23423
Affinity Capture-MS Homo sapiens
155 RAB11A 8766
Proximity Label-MS Homo sapiens
156 TMEM38B  
Affinity Capture-MS Homo sapiens
157 OSBPL5 114879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 EPHA2 1969
Proximity Label-MS Homo sapiens
159 DNAJC1 64215
Proximity Label-MS Homo sapiens
160 KRAS 3845
Proximity Label-MS Homo sapiens
161 RAB5C 5878
Proximity Label-MS Homo sapiens
162 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 SRP9 6726
Affinity Capture-MS Homo sapiens
164 CKAP4 10970
Proximity Label-MS Homo sapiens
165 S1PR4  
Affinity Capture-MS Homo sapiens
166 SPINT2 10653
Affinity Capture-MS Homo sapiens
167 RPA3 6119
Proximity Label-MS Homo sapiens
168 ESYT1 23344
Affinity Capture-MS Homo sapiens
169 ASB14  
Affinity Capture-MS Homo sapiens
170 DERL1 79139
Proximity Label-MS Homo sapiens
171 ARL4D  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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