Gene description for OTULIN
Gene name OTU deubiquitinase with linear linkage specificity
Gene symbol OTULIN
Other names/aliases FAM105B
GUM
Species Homo sapiens
 Database cross references - OTULIN
ExoCarta ExoCarta_90268
Vesiclepedia VP_90268
Entrez Gene 90268
HGNC 25118
MIM 615712
UniProt Q96BN8  
 OTULIN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for OTULIN
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 EXP
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 IMP
    protein binding GO:0005515 IPI
    cysteine-type peptidase activity GO:0008234 IMP
Biological Process
    sprouting angiogenesis GO:0002040 ISS
    proteolysis GO:0006508 IEA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IBA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IDA
    Wnt signaling pathway GO:0016055 IEA
    protein ubiquitination GO:0016567 TAS
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IDA
    innate immune response GO:0045087 IMP
    negative regulation of inflammatory response GO:0050728 IBA
    negative regulation of inflammatory response GO:0050728 IMP
    negative regulation of inflammatory response GO:0050728 ISS
    regulation of canonical Wnt signaling pathway GO:0060828 ISS
    nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070431 IMP
    protein linear deubiquitination GO:1990108 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    LUBAC complex GO:0071797 IEA
 Experiment description of studies that identified OTULIN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for OTULIN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFT2D1 113402
Two-hybrid Homo sapiens
2 TMEM239  
Two-hybrid Homo sapiens
3 LRRC15 131578
Affinity Capture-MS Homo sapiens
4 HK2 3099
Affinity Capture-MS Homo sapiens
5 SNX27 81609
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-fractionation Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 VIPR2  
Affinity Capture-MS Homo sapiens
8 CYB5B 80777
Affinity Capture-MS Homo sapiens
9 ATG13 9776
Affinity Capture-Western Homo sapiens
10 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CXCR4 7852
Affinity Capture-MS Homo sapiens
12 DVL2 1856
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 TP53 7157
Negative Genetic Homo sapiens
14 ICAM1 3383
Affinity Capture-MS Homo sapiens
15 TSC2 7249
Affinity Capture-MS Homo sapiens
16 TNF  
Affinity Capture-Western Homo sapiens
17 WRNIP1 56897
Affinity Capture-MS Homo sapiens
18 HCST  
Affinity Capture-MS Homo sapiens
19 DSG4 147409
Affinity Capture-MS Homo sapiens
20 OTUD7B 56957
Two-hybrid Homo sapiens
21 RNF31 55072
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 USP5 8078
Affinity Capture-MS Homo sapiens
23 GEMIN5 25929
Affinity Capture-MS Homo sapiens
24 SULT1C2 6819
Affinity Capture-MS Homo sapiens
25 BOP1 23246
Affinity Capture-MS Homo sapiens
26 KLRC4  
Affinity Capture-MS Homo sapiens
27 SUSD6 9766
Two-hybrid Homo sapiens
28 BNIP1 662
Affinity Capture-MS Homo sapiens
29 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
30 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
32 FAM168A  
Two-hybrid Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 BCL10  
Biochemical Activity Homo sapiens
35 HDAC6 10013
Affinity Capture-MS Homo sapiens
36 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
37 CD80 941
Affinity Capture-MS Homo sapiens
38 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 SLC18A2  
Affinity Capture-MS Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 TRIM32 22954
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 RMDN3 55177
Affinity Capture-MS Homo sapiens
43 DUSP9  
Affinity Capture-MS Homo sapiens
44 VCP 7415
Co-fractionation Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
45 SELENBP1 8991
Affinity Capture-MS Homo sapiens
46 SLC27A4 10999
Affinity Capture-MS Homo sapiens
47 USP3 9960
Affinity Capture-MS Homo sapiens
48 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
49 NEDD8 4738
Affinity Capture-MS Homo sapiens
50 PUM1 9698
Affinity Capture-MS Homo sapiens
51 SPG20 23111
Two-hybrid Homo sapiens
52 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SHARPIN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 DSTN 11034
Co-fractionation Homo sapiens
55 MALT1 10892
Affinity Capture-Western Homo sapiens
56 SNW1 22938
Affinity Capture-MS Homo sapiens
57 OSBPL8 114882
Affinity Capture-MS Homo sapiens
58 KLRC1  
Affinity Capture-MS Homo sapiens
59 PDZK1 5174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 UCHL3 7347
Affinity Capture-MS Homo sapiens
61 BRAP  
Affinity Capture-MS Homo sapiens
62 ATP2C1 27032
Affinity Capture-MS Homo sapiens
63 INSRR 3645
Affinity Capture-MS Homo sapiens
64 RBCK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
65 DUSP23 54935
Affinity Capture-MS Homo sapiens
66 B3GALT5  
Affinity Capture-MS Homo sapiens
67 SLC2A9  
Affinity Capture-MS Homo sapiens
68 CUL4A 8451
Affinity Capture-MS Homo sapiens
69 HYPM  
Affinity Capture-MS Homo sapiens
70 GNL3L 54552
Affinity Capture-MS Homo sapiens
71 SYNJ2BP 55333
Affinity Capture-MS Homo sapiens
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