Gene description for OTUD7B
Gene name OTU deubiquitinase 7B
Gene symbol OTUD7B
Other names/aliases CEZANNE
ZA20D1
Species Homo sapiens
 Database cross references - OTUD7B
ExoCarta ExoCarta_56957
Entrez Gene 56957
HGNC 16683
MIM 611748
UniProt Q6GQQ9  
 OTUD7B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for OTUD7B
Molecular Function
    Lys48-specific deubiquitinase activity GO:1990380 IDA
    DNA binding GO:0003677 IEA
    zinc ion binding GO:0008270 IEA
    ubiquitin-specific protease activity GO:0004843 IDA
    protein binding GO:0005515 IPI
    cysteine-type peptidase activity GO:0008234 ISS
    K63-linked polyubiquitin binding GO:0070530 IEA
Biological Process
    protein K11-linked deubiquitination GO:0035871 IDA
    negative regulation of protein localization to nucleus GO:1900181 IDA
    mucosal immune response GO:0002385 ISS
    protein K63-linked deubiquitination GO:0070536 IDA
    negative regulation of interleukin-8 production GO:0032717 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    protein K48-linked deubiquitination GO:0071108 ISS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified OTUD7B in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OTUD7B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAF6 7189
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which OTUD7B is involved
PathwayEvidenceSource
TNFR1-mediated proapoptotic signaling TAS Reactome





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