Gene description for TRAF6
Gene name TNF receptor-associated factor 6, E3 ubiquitin protein ligase
Gene symbol TRAF6
Other names/aliases MGC:3310
RNF85
Species Homo sapiens
 Database cross references - TRAF6
ExoCarta ExoCarta_7189
Vesiclepedia VP_7189
Entrez Gene 7189
HGNC 12036
MIM 602355
UniProt Q9Y4K3  
 TRAF6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TRAF6
Molecular Function
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IPI
    thioesterase binding GO:0031996 IPI
    ligase activity GO:0016874 IEA
    tumor necrosis factor receptor binding GO:0005164 IPI
    protein kinase binding GO:0019901 IPI
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    ubiquitin conjugating enzyme binding GO:0031624 IDA
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein kinase B binding GO:0043422 IPI
    histone deacetylase binding GO:0042826 IPI
    protein N-terminus binding GO:0047485 IPI
Biological Process
    protein K63-linked ubiquitination GO:0070534 IGI
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 TAS
    apoptotic signaling pathway GO:0097190 TAS
    myeloid dendritic cell differentiation GO:0043011 IEA
    positive regulation of transcription regulatory region DNA binding GO:2000679 IDA
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IEA
    cell development GO:0048468 IEA
    neural tube closure GO:0001843 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    positive regulation of protein ubiquitination GO:0031398 NAS
    regulation of immunoglobulin secretion GO:0051023 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IMP
    osteoclast differentiation GO:0030316 IEA
    positive regulation of JUN kinase activity GO:0043507 NAS
    positive regulation of interleukin-6 biosynthetic process GO:0045410 IEA
    negative regulation of apoptotic process GO:0043066 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 NAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 IMP
    protein polyubiquitination GO:0000209 IDA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    activation of MAPK activity GO:0000187 TAS
    positive regulation of interleukin-12 biosynthetic process GO:0045084 IEA
    activation of protein kinase activity GO:0032147 IDA
    T-helper 1 type immune response GO:0042088 IEA
    positive regulation of T cell activation GO:0050870 IC
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IEA
    positive regulation of T cell proliferation GO:0042102 IEA
    ossification GO:0001503 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    interleukin-1-mediated signaling pathway GO:0070498 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    bone resorption GO:0045453 IEA
    positive regulation of interleukin-2 production GO:0032743 IMP
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    positive regulation of osteoclast differentiation GO:0045672 IDA
    innate immune response GO:0045087 TAS
    response to interleukin-1 GO:0070555 IDA
    JNK cascade GO:0007254 TAS
    protein autoubiquitination GO:0051865 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    membrane protein intracellular domain proteolysis GO:0031293 TAS
    cellular response to lipopolysaccharide GO:0071222 IDA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    stress-activated MAPK cascade GO:0051403 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    protein complex assembly GO:0006461 IEA
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    positive regulation of T cell cytokine production GO:0002726 IMP
    positive regulation of apoptotic process GO:0043065 TAS
    I-kappaB kinase/NF-kappaB signaling GO:0007249 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
Subcellular Localization
    CD40 receptor complex GO:0035631 ISS
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    cytoplasmic side of plasma membrane GO:0009898 ISS
    lipid particle GO:0005811 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    endosome membrane GO:0010008 TAS
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 TAS
    mitochondrion GO:0005739 IDA
    cell cortex GO:0005938 IEA
    nucleolus GO:0005730 IDA
    protein complex GO:0043234 IDA
 Experiment description of studies that identified TRAF6 in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TRAF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Invivo Homo sapiens
2 TNFAIP3 7128
Invivo Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 CD40 958
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 ZFAND5 7763
Affinity Capture-Western Homo sapiens
6 MAP3K14  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 IRAK2  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
8 TRAF3IP2  
Affinity Capture-MS Homo sapiens
9 IL17RB  
Invivo Homo sapiens
10 XIAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 TRAF2 7186
Two-hybrid Homo sapiens
12 PSMD12 5718
Affinity Capture-MS Homo sapiens
13 NASP 4678
Affinity Capture-MS Homo sapiens
14 ZNF675  
Invivo Homo sapiens
Two-hybrid Homo sapiens
15 TAX1BP1 8887
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 MAP3K7IP2  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
17 ASXL2  
Affinity Capture-MS Homo sapiens
18 PSMD13 5719
Affinity Capture-MS Homo sapiens
19 DDX3X 1654
Affinity Capture-MS Homo sapiens
20 IRAK4 51135
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
21 PAICS 10606
Affinity Capture-MS Homo sapiens
22 EPPK1 83481
Affinity Capture-MS Homo sapiens
23 PRKCZ 5590
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 ALS2CR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
26 TAB1 10454
Invivo Homo sapiens
27 IRAK1  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
28 PSMC3 5702
Affinity Capture-MS Homo sapiens
29 UBB 7314
Affinity Capture-MS Homo sapiens
30 KCNQ1  
Affinity Capture-MS Homo sapiens
31 TAB3 257397
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
32 MAP3K7  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
33 EIF4E2  
Affinity Capture-MS Homo sapiens
34 TMEM189-UBE2V1 387522
Invivo Homo sapiens
Invitro Homo sapiens
35 PSMC1 5700
Affinity Capture-MS Homo sapiens
36 PSMD7 5713
Affinity Capture-MS Homo sapiens
37 SPOP  
Invitro Homo sapiens
38 MAST2  
Affinity Capture-Western Homo sapiens
39 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 TNFRSF11A  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
41 TRIM37  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
42 GANAB 23193
Affinity Capture-MS Homo sapiens
43 EDA2R  
Invivo Homo sapiens
Invitro Homo sapiens
44 NGFR 4804
Affinity Capture-Western Homo sapiens
45 RIPK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
46 SIGIRR 59307
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 USP7 7874
Invitro Homo sapiens
48 TIFA  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
49 MTHFD1 4522
Affinity Capture-MS Homo sapiens
50 PSMB5 5693
Affinity Capture-MS Homo sapiens
51 SRC 6714
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
52 CTPS1 1503
Affinity Capture-MS Homo sapiens
53 TDP2 51567
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 VDAC1 7416
Affinity Capture-MS Homo sapiens
55 SYK 6850
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
56 TNFRSF19  
Invivo Homo sapiens
57 PSMD1 5707
Affinity Capture-MS Homo sapiens
58 IARS 3376
Affinity Capture-MS Homo sapiens
59 TJP2 9414
Affinity Capture-MS Homo sapiens
60 PSMC2 5701
Affinity Capture-MS Homo sapiens
61 VCP 7415
Affinity Capture-MS Homo sapiens
62 OTUD7B 56957
Invitro Homo sapiens
Invivo Homo sapiens
63 TARS 6897
Affinity Capture-MS Homo sapiens
64 IRAK3 11213
Affinity Capture-Western Homo sapiens
65 VARS2  
Affinity Capture-MS Homo sapiens
66 PFAS 5198
Affinity Capture-MS Homo sapiens
67 ECSIT 51295
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
68 PSMD3 5709
Affinity Capture-MS Homo sapiens
69 TNFRSF13B  
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which TRAF6 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Downstream TCR signaling TAS Reactome
FCERI mediated NF-kB activation TAS Reactome
IKK complex recruitment mediated by RIP1 TAS Reactome
Interleukin-1 signaling TAS Reactome
IRAK1 recruits IKK complex TAS Reactome
IRAK1 recruits IKK complex TAS Reactome
IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation TAS Reactome
IRAK2 mediated activation of TAK1 complex TAS Reactome
IRAK2 mediated activation of TAK1 complex TAS Reactome
IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88:Mal cascade initiated on plasma membrane TAS Reactome
NF-kB is activated and signals survival TAS Reactome
NOD1/2 Signaling Pathway TAS Reactome
NRIF signals cell death from the nucleus TAS Reactome
p75NTR recruits signalling complexes TAS Reactome
Regulated proteolysis of p75NTR TAS Reactome
TAK1 activates NFkB by phosphorylation and activation of IKKs complex TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRAF6 mediated induction of TAK1 complex TAS Reactome
TRAF6 mediated IRF7 activation TAS Reactome
TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling TAS Reactome
TRAF6 mediated NF-kB activation TAS Reactome





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