Gene description for STK26
Gene name serine/threonine protein kinase 26
Gene symbol STK26
Other names/aliases MASK
MST4
Species Homo sapiens
 Database cross references - STK26
ExoCarta ExoCarta_51765
Entrez Gene 51765
HGNC 18174
MIM 300547
UniProt Q9P289  
 STK26 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for STK26
Molecular Function
    identical protein binding GO:0042802 IPI
    protein kinase activity GO:0004672 IMP
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein homodimerization activity GO:0042803 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
Biological Process
    signal transduction by protein phosphorylation GO:0023014 IBA
    programmed cell death GO:0012501 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    regulation of hydrogen peroxide-induced cell death GO:1903205 IMP
    apoptotic process GO:0006915 TAS
    regulation of mitotic cell cycle GO:0007346 IBA
    regulation of apoptotic process GO:0042981 IDA
    microvillus assembly GO:0030033 IDA
    protein phosphorylation GO:0006468 IMP
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    activation of protein kinase activity GO:0032147 IBA
    protein autophosphorylation GO:0046777 IMP
    response to hydrogen peroxide GO:0042542 IDA
Subcellular Localization
    apical plasma membrane GO:0016324 IDA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 IDA
    Golgi membrane GO:0000139 IEA
    cell periphery GO:0071944 IDA
    Golgi apparatus GO:0005794 IDA
    nucleoplasm GO:0005654 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    Golgi-associated vesicle GO:0005798 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    centrosome GO:0005813 IDA
 Experiment description of studies that identified STK26 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STK26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Affinity Capture-MS Homo sapiens
2 STRN4 29888
Affinity Capture-MS Homo sapiens
3 STRIP1 85369
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 RAF1 5894
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 SLMAP  
Affinity Capture-MS Homo sapiens
6 EPHB2 2048
Phenotypic Enhancement Homo sapiens
7 PDCD10 11235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 STRN3 29966
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 STRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
11 FGFR1OP2  
Affinity Capture-MS Homo sapiens
12 FAM40B  
Affinity Capture-MS Homo sapiens
13 DKFZp547A023  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 CTTNBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FLJ21168  
Affinity Capture-MS Homo sapiens
16 PREI3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which STK26 is involved
PathwayEvidenceSource
Apoptotic cleavage of cellular proteins TAS Reactome





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