Gene description for SRP54
Gene name signal recognition particle 54kDa
Gene symbol SRP54
Other names/aliases -
Species Homo sapiens
 Database cross references - SRP54
ExoCarta ExoCarta_6729
Vesiclepedia VP_6729
Entrez Gene 6729
HGNC 11301
MIM 604857
UniProt P61011  
 SRP54 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SRP54
Molecular Function
    RNA binding GO:0003723 HDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    7S RNA binding GO:0008312 IBA
    7S RNA binding GO:0008312 IDA
    ATP hydrolysis activity GO:0016887 IEA
    GDP binding GO:0019003 IDA
    endoplasmic reticulum signal peptide binding GO:0030942 IBA
    endoplasmic reticulum signal peptide binding GO:0030942 IDA
    ribonucleoprotein complex binding GO:0043021 IDA
Biological Process
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 IC
    SRP-dependent cotranslational protein targeting to membrane, translocation GO:0006616 IBA
    SRP-dependent cotranslational protein targeting to membrane, translocation GO:0006616 ISS
    SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition GO:0006617 ISS
    neutrophil chemotaxis GO:0030593 ISS
    granulocyte differentiation GO:0030851 IMP
    exocrine pancreas development GO:0031017 ISS
    protein targeting to ER GO:0045047 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IEA
    signal recognition particle, endoplasmic reticulum targeting GO:0005786 IBA
    signal recognition particle, endoplasmic reticulum targeting GO:0005786 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified SRP54 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRP54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SRP19 6728
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 EIF2S3 1968
Affinity Capture-MS Homo sapiens
6 SRPRB 58477
Affinity Capture-MS Homo sapiens
7 CSE1L 1434
Affinity Capture-MS Homo sapiens
8 PTPN1 5770
Proximity Label-MS Homo sapiens
9 STX4 6810
Proximity Label-MS Homo sapiens
10 CCDC102B  
Affinity Capture-MS Homo sapiens
11 PRKDC 5591
Co-fractionation Homo sapiens
12 CHDC2  
Affinity Capture-MS Homo sapiens
13 MRPS18C  
Affinity Capture-MS Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 CAPZB 832
Affinity Capture-MS Homo sapiens
16 TOP2A 7153
Affinity Capture-MS Homo sapiens
17 CPLX2 10814
Affinity Capture-MS Homo sapiens
18 B3GAT1  
Proximity Label-MS Homo sapiens
19 HSPA2 3306
Affinity Capture-MS Homo sapiens
20 METAP2 10988
Affinity Capture-MS Homo sapiens
21 NPM1 4869
Affinity Capture-MS Homo sapiens
22 SSR1 6745
Proximity Label-MS Homo sapiens
23 SMC2 10592
Co-fractionation Homo sapiens
24 GSPT1 2935
Affinity Capture-MS Homo sapiens
25 SRP68 6730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
26 MECP2 4204
Affinity Capture-MS Homo sapiens
27 RPL4 6124
Affinity Capture-MS Homo sapiens
28 RPS11 6205
Affinity Capture-MS Homo sapiens
29 RPL19 6143
Affinity Capture-MS Homo sapiens
30 CTCF  
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SMC4 10051
Co-fractionation Homo sapiens
33 TPT1 7178
Affinity Capture-MS Homo sapiens
34 XRCC4  
Co-fractionation Homo sapiens
35 LRRC59 55379
Proximity Label-MS Homo sapiens
36 GEMIN5 25929
Affinity Capture-Western Homo sapiens
37 SRP9 6726
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 EMD 2010
Proximity Label-MS Homo sapiens
39 SRP14 6727
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 TARBP2  
Affinity Capture-MS Homo sapiens
42 ALDH5A1 7915
Affinity Capture-MS Homo sapiens
43 DNAJC2 27000
Affinity Capture-MS Homo sapiens
44 MAPRE1 22919
Affinity Capture-MS Homo sapiens
45 DNAJC5 80331
Proximity Label-MS Homo sapiens
46 RPL5 6125
Affinity Capture-MS Homo sapiens
47 DDX20 11218
Affinity Capture-Western Homo sapiens
48 Srp72  
Affinity Capture-MS Mus musculus
49 OGT 8473
Reconstituted Complex Homo sapiens
50 RPL31 6160
Proximity Label-MS Homo sapiens
51 SF3B6 51639
Co-fractionation Homo sapiens
52 RPA4  
Proximity Label-MS Homo sapiens
53 GEMIN2 8487
Affinity Capture-Western Homo sapiens
54 DHX9 1660
Affinity Capture-MS Homo sapiens
55 MRPL11 65003
Affinity Capture-MS Homo sapiens
56 MAGEB2 4113
Affinity Capture-MS Homo sapiens
57 CEBPA  
Protein-peptide Homo sapiens
58 SEC61B 10952
Proximity Label-MS Homo sapiens
59 HMGB2 3148
Affinity Capture-MS Homo sapiens
60 DUSP22  
Affinity Capture-MS Homo sapiens
61 ATG4B 23192
Affinity Capture-MS Homo sapiens
62 GIMAP8 155038
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 XPO1 7514
Affinity Capture-MS Homo sapiens
64 EGLN3  
Affinity Capture-MS Homo sapiens
65 BAG2 9532
Affinity Capture-MS Homo sapiens
66 NTRK1 4914
Affinity Capture-MS Homo sapiens
67 MRPS11  
Affinity Capture-MS Homo sapiens
68 TFAP4 7023
Co-fractionation Homo sapiens
69 STIM1 6786
Proximity Label-MS Homo sapiens
70 SSB 6741
Affinity Capture-MS Homo sapiens
71 FKBP8 23770
Proximity Label-MS Homo sapiens
72 SF3A1 10291
Co-fractionation Homo sapiens
73 DMAP1 55929
Affinity Capture-MS Homo sapiens
74 GEMIN4 50628
Affinity Capture-Western Homo sapiens
75 NCAPH 23397
Affinity Capture-MS Homo sapiens
76 PSPC1 55269
Affinity Capture-MS Homo sapiens
77 RPA3 6119
Proximity Label-MS Homo sapiens
78 EGFR 1956
Negative Genetic Homo sapiens
79 SNW1 22938
Affinity Capture-MS Homo sapiens
80 Rpl35 66489
Affinity Capture-MS Mus musculus
81 RNF185  
Affinity Capture-MS Homo sapiens
82 RPS16 6217
Affinity Capture-MS Homo sapiens
83 RBM8A 9939
Affinity Capture-MS Homo sapiens
84 SMN1 6606
Affinity Capture-Western Homo sapiens
85 RPS21 6227
Cross-Linking-MS (XL-MS) Homo sapiens
86 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
87 G3BP1 10146
Affinity Capture-MS Homo sapiens
88 SRP72 6731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
90 SRP54 6729
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
91 STK26 51765
Affinity Capture-MS Homo sapiens
92 G3BP2 9908
Affinity Capture-MS Homo sapiens
93 SEC62 7095
Proximity Label-MS Homo sapiens
94 DDR1 780
Affinity Capture-MS Homo sapiens
95 MND1  
Affinity Capture-MS Homo sapiens
96 CIT 11113
Affinity Capture-MS Homo sapiens
97 DNAJC1 64215
Proximity Label-MS Homo sapiens
98 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
99 CKAP4 10970
Proximity Label-MS Homo sapiens
100 CDC5L 988
Affinity Capture-MS Homo sapiens
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