Gene description for SRP54
Gene name signal recognition particle 54kDa
Gene symbol SRP54
Other names/aliases -
Species Homo sapiens
 Database cross references - SRP54
ExoCarta ExoCarta_6729
Entrez Gene 6729
HGNC 11301
MIM 604857
UniProt P61011  
 SRP54 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SRP54
Molecular Function
    GTP binding GO:0005525 IDA
    ribonucleoprotein complex binding GO:0043021 IDA
    GDP binding GO:0019003 IDA
    GTPase activity GO:0003924 IDA
    poly(A) RNA binding GO:0044822 IDA
    endoplasmic reticulum signal peptide binding GO:0030942 IDA
    7S RNA binding GO:0008312 IDA
    drug binding GO:0008144 IDA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition GO:0006617 ISS
    SRP-dependent cotranslational protein targeting to membrane, translocation GO:0006616 ISS
    response to drug GO:0042493 IDA
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    translation GO:0006412 TAS
    protein targeting to ER GO:0045047 IMP
    gene expression GO:0010467 TAS
Subcellular Localization
    nuclear speck GO:0016607 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    nucleolus GO:0005730 IDA
    signal recognition particle, endoplasmic reticulum targeting GO:0005786 IDA
 Experiment description of studies that identified SRP54 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRP54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRP54 6729
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
2 SRP19  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SRP54 is involved
PathwayEvidenceSource
SRP-dependent cotranslational protein targeting to membrane TAS Reactome





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