Gene description for DDR1
Gene name discoidin domain receptor tyrosine kinase 1
Gene symbol DDR1
Other names/aliases CAK
CD167
DDR
EDDR1
HGK2
MCK10
NEP
NTRK4
PTK3
PTK3A
RTK6
TRKE
Species Homo sapiens
 Database cross references - DDR1
ExoCarta ExoCarta_780
Vesiclepedia VP_780
Entrez Gene 780
HGNC 2730
MIM 600408
UniProt Q08345  
 DDR1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for DDR1
Molecular Function
    protein tyrosine kinase collagen receptor activity GO:0038062 IDA
    protein binding GO:0005515 IPI
    transmembrane receptor protein tyrosine kinase activity GO:0004714 TAS
    metal ion binding GO:0046872 IEA
    collagen binding GO:0005518 IMP
    ATP binding GO:0005524 IEA
Biological Process
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    regulation of extracellular matrix disassembly GO:0010715 IMP
    mammary gland alveolus development GO:0060749 IEA
    extracellular matrix organization GO:0030198 TAS
    ear development GO:0043583 IEA
    protein autophosphorylation GO:0046777 IDA
    negative regulation of cell proliferation GO:0008285 IEA
    wound healing, spreading of cells GO:0044319 IMP
    regulation of cell growth GO:0001558 IEA
    smooth muscle cell-matrix adhesion GO:0061302 IMP
    smooth muscle cell migration GO:0014909 IMP
    embryo implantation GO:0007566 IEA
    regulation of cell-matrix adhesion GO:0001952 IEA
    cell adhesion GO:0007155 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IDA
    lactation GO:0007595 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IMP
Subcellular Localization
    integral component of plasma membrane GO:0005887 IEA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified DDR1 in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
EV Enriched markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 66
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix|CD63|CD81
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for DDR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNAPIN 23557
Two-hybrid Homo sapiens
2 SNRNP40 9410
Two-hybrid Homo sapiens
3 TM4SF1 4071
Two-hybrid Homo sapiens
4 TTR 7276
Two-hybrid Homo sapiens
5 RGS2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DDR1 is involved
PathwayEvidenceSource
Non-integrin membrane-ECM interactions TAS Reactome





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