Gene description for DDR1
Gene name discoidin domain receptor tyrosine kinase 1
Gene symbol DDR1
Other names/aliases CAK
CD167
DDR
EDDR1
HGK2
MCK10
NEP
NTRK4
PTK3
PTK3A
RTK6
TRKE
Species Homo sapiens
 Database cross references - DDR1
ExoCarta ExoCarta_780
Vesiclepedia VP_780
Entrez Gene 780
HGNC 2730
MIM 600408
UniProt Q08345  
 DDR1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for DDR1
Molecular Function
    transmembrane receptor protein tyrosine kinase activity GO:0004714 TAS
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IBA
    collagen binding GO:0005518 IDA
    collagen binding GO:0005518 IMP
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IBA
    protein tyrosine kinase collagen receptor activity GO:0038062 IDA
    metal ion binding GO:0046872 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    regulation of cell growth GO:0001558 IEA
    regulation of cell-matrix adhesion GO:0001952 IEA
    chromatin remodeling GO:0006338 IEA
    cell adhesion GO:0007155 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    embryo implantation GO:0007566 IEA
    lactation GO:0007595 IEA
    cell population proliferation GO:0008283 IEA
    negative regulation of cell population proliferation GO:0008285 IEA
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    regulation of extracellular matrix disassembly GO:0010715 IMP
    positive regulation of neuron projection development GO:0010976 IBA
    smooth muscle cell migration GO:0014909 IMP
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IDA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IMP
    peptidyl-tyrosine autophosphorylation GO:0038083 IDA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    ear development GO:0043583 IEA
    wound healing, spreading of cells GO:0044319 IMP
    protein autophosphorylation GO:0046777 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    mammary gland alveolus development GO:0060749 IEA
    smooth muscle cell-matrix adhesion GO:0061302 IMP
    axon development GO:0061564 IEA
    neuron projection extension GO:1990138 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified DDR1 in exosomes
1
Experiment ID 76
MISEV standards
EM
Biophysical techniques
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for DDR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM4 4173
Affinity Capture-MS Homo sapiens
2 UPK2 7379
Affinity Capture-MS Homo sapiens
3 PCDHA8  
Affinity Capture-MS Homo sapiens
4 SGCA  
Affinity Capture-MS Homo sapiens
5 SRP54 6729
Affinity Capture-MS Homo sapiens
6 TMEM200A 114801
Affinity Capture-MS Homo sapiens
7 DRG2 1819
Affinity Capture-MS Homo sapiens
8 TIGIT  
Affinity Capture-MS Homo sapiens
9 CDH2 1000
Affinity Capture-Western Homo sapiens
10 IZUMO1  
Affinity Capture-MS Homo sapiens
11 ERBB2 2064
PCA Homo sapiens
12 ITIH2 3698
Affinity Capture-MS Homo sapiens
13 TGFBR2 7048
Affinity Capture-MS Homo sapiens
14 SHC1 6464
Reconstituted Complex Homo sapiens
15 CLDND1 56650
Affinity Capture-MS Homo sapiens
16 RNF11 26994
Affinity Capture-MS Homo sapiens
17 OPALIN  
Affinity Capture-MS Homo sapiens
18 CD83  
Affinity Capture-MS Homo sapiens
19 WWOX 51741
Affinity Capture-MS Homo sapiens
20 TNFSF8  
Affinity Capture-MS Homo sapiens
21 PCDHAC1  
Affinity Capture-MS Homo sapiens
22 DLL1  
Affinity Capture-MS Homo sapiens
23 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
24 UBQLN2 29978
Two-hybrid Homo sapiens
25 TTR 7276
Two-hybrid Homo sapiens
26 TSPAN33 340348
Affinity Capture-MS Homo sapiens
27 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MYCT1 80177
Affinity Capture-MS Homo sapiens
29 CDH1 999
Affinity Capture-Western Homo sapiens
30 TSPAN15 23555
Affinity Capture-MS Homo sapiens
31 RGS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 EFNB1 1947
Affinity Capture-MS Homo sapiens
33 SHISA3  
Affinity Capture-MS Homo sapiens
34 PCDHA9  
Affinity Capture-MS Homo sapiens
35 ACVR2B  
Affinity Capture-MS Homo sapiens
36 RNMT 8731
Co-fractionation Homo sapiens
37 CEACAM21  
Affinity Capture-MS Homo sapiens
38 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
39 ERBB3 2065
Co-fractionation Homo sapiens
40 KCNE3  
Affinity Capture-MS Homo sapiens
41 FFAR1  
Affinity Capture-MS Homo sapiens
42 TMEM150A  
Affinity Capture-MS Homo sapiens
43 SPACA1 81833
Affinity Capture-MS Homo sapiens
44 RAD50 10111
Affinity Capture-MS Homo sapiens
45 NXF1 10482
Affinity Capture-RNA Homo sapiens
46 VSIG4  
Affinity Capture-MS Homo sapiens
47 SNRNP40 9410
Two-hybrid Homo sapiens
48 KIAA1429 25962
Affinity Capture-MS Homo sapiens
49 MANSC1 54682
Affinity Capture-MS Homo sapiens
50 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MAPK6  
Two-hybrid Homo sapiens
52 DDR2 4921
Affinity Capture-MS Homo sapiens
53 MOV10 4343
Affinity Capture-RNA Homo sapiens
54 LSMEM2  
Affinity Capture-MS Homo sapiens
55 INSRR 3645
Affinity Capture-MS Homo sapiens
56 SNAPIN 23557
Two-hybrid Homo sapiens
57 RELT  
Affinity Capture-MS Homo sapiens
58 C16orf54 283897
Affinity Capture-MS Homo sapiens
59 FAM115C  
Affinity Capture-MS Homo sapiens
60 FLRT1  
Affinity Capture-MS Homo sapiens
61 KIAA1644  
Affinity Capture-MS Homo sapiens
62 ITGB4 3691
Co-fractionation Homo sapiens
63 FLOT2 2319
Affinity Capture-MS Homo sapiens
64 TMEM231 79583
Affinity Capture-MS Homo sapiens
65 EPHA2 1969
Affinity Capture-MS Homo sapiens
66 DLK1  
Affinity Capture-MS Homo sapiens
67 NUDT3 11165
Two-hybrid Homo sapiens
68 EVA1B 55194
Affinity Capture-MS Homo sapiens
69 TSPAN17 26262
Affinity Capture-MS Homo sapiens
70 SLC31A1 1317
Affinity Capture-MS Homo sapiens
71 ITM2A  
Affinity Capture-MS Homo sapiens
72 MRAP2  
Affinity Capture-MS Homo sapiens
73 KCNC3 3748
Affinity Capture-MS Homo sapiens
74 NOTCH1 4851
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
75 GPR83  
Affinity Capture-MS Homo sapiens
76 KCNE4  
Affinity Capture-MS Homo sapiens
77 TM4SF1 4071
Two-hybrid Homo sapiens
78 BCR 613
Affinity Capture-MS Homo sapiens
79 SYT11 23208
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DDR1 is involved
PathwayEvidenceSource
Extracellular matrix organization TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome





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