Gene description for RNMT
Gene name RNA (guanine-7-) methyltransferase
Gene symbol RNMT
Other names/aliases CMT1
CMT1c
MET
Met
RG7MT1
cm1p
hCMT1
hMet
Species Homo sapiens
 Database cross references - RNMT
ExoCarta ExoCarta_8731
Vesiclepedia VP_8731
Entrez Gene 8731
HGNC 10075
MIM 603514
UniProt O43148  
 RNMT identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RNMT
Molecular Function
    RNA binding GO:0003723 IMP
    mRNA 5'-cap (guanine-N7-)-methyltransferase activity GO:0004482 IBA
    mRNA 5'-cap (guanine-N7-)-methyltransferase activity GO:0004482 IDA
    mRNA 5'-cap (guanine-N7-)-methyltransferase activity GO:0004482 IMP
    mRNA 5'-cap (guanine-N7-)-methyltransferase activity GO:0004482 TAS
    protein binding GO:0005515 IPI
Biological Process
    7-methylguanosine mRNA capping GO:0006370 IBA
    7-methylguanosine mRNA capping GO:0006370 IDA
    7-methylguanosine mRNA capping GO:0006370 IMP
    7-methylguanosine mRNA capping GO:0006370 TAS
    RNA 5'-cap (guanine-N7)-methylation GO:0106005 IEA
    cellular response to leukemia inhibitory factor GO:1990830 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mRNA capping enzyme complex GO:0031533 IDA
    receptor complex GO:0043235 IDA
    mRNA cap methyltransferase RNMT:RAMAC complex GO:0160130 IDA
 Experiment description of studies that identified RNMT in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RNMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RNGTT 8732
Reconstituted Complex Homo sapiens
3 SRM 6723
Co-fractionation Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 DDR1 780
Co-fractionation Homo sapiens
6 PLEKHA3  
Two-hybrid Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 CUL1 8454
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 MYCN  
Affinity Capture-MS Homo sapiens
11 NCBP1 4686
Co-fractionation Homo sapiens
12 RXRA 6256
Co-fractionation Homo sapiens
13 CLINT1 9685
Co-fractionation Homo sapiens
14 NR4A1  
Affinity Capture-MS Homo sapiens
15 Fam103a1  
Two-hybrid Mus musculus
16 KPNA1 3836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NCBP2 22916
Co-fractionation Homo sapiens
18 CCT2 10576
Co-fractionation Homo sapiens
19 AGO2 27161
Co-fractionation Homo sapiens
20 LONP1 9361
Co-fractionation Homo sapiens
21 ITGB1 3688
Co-fractionation Homo sapiens
22 KPNB1 3837
Affinity Capture-MS Homo sapiens
23 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DLD 1738
Co-fractionation Homo sapiens
25 CUL5 8065
Affinity Capture-MS Homo sapiens
26 KPNA6 23633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 TRADD 8717
Co-fractionation Homo sapiens
29 EGFR 1956
Negative Genetic Homo sapiens
30 KPNA2 3838
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
31 CAND1 55832
Affinity Capture-MS Homo sapiens
32 EIF4E 1977
Co-fractionation Homo sapiens
33 EPPK1 83481
Co-fractionation Homo sapiens
34 KIF23 9493
Co-fractionation Homo sapiens
35 EPRS 2058
Co-fractionation Homo sapiens
36 ARHGAP1 392
Co-fractionation Homo sapiens
37 EIF4E2  
Co-fractionation Homo sapiens
38 APP 351
Co-fractionation Homo sapiens
39 CCT7 10574
Co-fractionation Homo sapiens
40 HEXA 3073
Co-fractionation Homo sapiens
41 COG2 22796
Co-fractionation Homo sapiens
42 KPNA4 3840
Affinity Capture-MS Homo sapiens
43 DPYSL2 1808
Co-fractionation Homo sapiens
44 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
45 APPL1 26060
Co-fractionation Homo sapiens
46 MYC  
Affinity Capture-MS Homo sapiens
47 HECW2  
Affinity Capture-MS Homo sapiens
48 HNRNPH3 3189
Co-fractionation Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 MCM2 4171
Affinity Capture-MS Homo sapiens
51 NT5DC3 51559
Co-fractionation Homo sapiens
52 SMAD3 4088
Co-fractionation Homo sapiens
53 ERBB3 2065
Co-fractionation Homo sapiens
54 ITGB4 3691
Co-fractionation Homo sapiens
55 RPRD1B 58490
Co-fractionation Homo sapiens
56 COPS5 10987
Affinity Capture-MS Homo sapiens
57 RIPK1 8737
Co-fractionation Homo sapiens
58 KRAS 3845
Negative Genetic Homo sapiens
59 NFIC 4782
Co-fractionation Homo sapiens
60 AR 367
Proximity Label-MS Homo sapiens
61 HEXB 3074
Co-fractionation Homo sapiens
62 CUL2 8453
Affinity Capture-MS Homo sapiens
63 MAT2B 27430
Co-fractionation Homo sapiens
64 KPNA5 3841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PRMT1 3276
Affinity Capture-MS Homo sapiens
66 EPS15L1 58513
Co-fractionation Homo sapiens
67 NFATC2IP  
Co-fractionation Homo sapiens
68 TRPC4AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 FAM103A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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