Gene description for HEXB
Gene name hexosaminidase B (beta polypeptide)
Gene symbol HEXB
Other names/aliases ENC-1AS
HEL-248
Species Homo sapiens
 Database cross references - HEXB
ExoCarta ExoCarta_3074
Entrez Gene 3074
HGNC 4879
MIM 606873
UniProt P07686  
 HEXB identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HEXB
Molecular Function
    protein heterodimerization activity GO:0046982 IDA
    beta-N-acetylhexosaminidase activity GO:0004563 IEA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    hyaluronan catabolic process GO:0030214 TAS
    hyaluronan metabolic process GO:0030212 TAS
    lipid storage GO:0019915 IEA
    carbohydrate metabolic process GO:0005975 TAS
    sensory perception of sound GO:0007605 IEA
    small molecule metabolic process GO:0044281 TAS
    glycosphingolipid metabolic process GO:0006687 TAS
    locomotory behavior GO:0007626 IEA
    skeletal system development GO:0001501 IEA
    lysosome organization GO:0007040 IEA
    male courtship behavior GO:0008049 IEA
    myelination GO:0042552 IEA
    keratan sulfate catabolic process GO:0042340 TAS
    regulation of cell shape GO:0008360 IEA
    phospholipid biosynthetic process GO:0008654 IEA
    ganglioside catabolic process GO:0006689 IEA
    oligosaccharide catabolic process GO:0009313 IEA
    chondroitin sulfate metabolic process GO:0030204 TAS
    neuromuscular process controlling balance GO:0050885 IEA
    glycosaminoglycan metabolic process GO:0030203 TAS
    cellular protein metabolic process GO:0044267 IEA
    penetration of zona pellucida GO:0007341 IEA
    cellular calcium ion homeostasis GO:0006874 IEA
    sphingolipid metabolic process GO:0006665 TAS
    astrocyte cell migration GO:0043615 IEA
    keratan sulfate metabolic process GO:0042339 TAS
    oogenesis GO:0048477 IEA
    chondroitin sulfate catabolic process GO:0030207 TAS
Subcellular Localization
    lysosomal lumen GO:0043202 TAS
    membrane GO:0016020 IDA
    acrosomal vesicle GO:0001669 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified HEXB in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HEXB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HEXB 3074
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
2 EIF2D 1939
Invitro Homo sapiens
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