Gene description for HEXA
Gene name hexosaminidase A (alpha polypeptide)
Gene symbol HEXA
Other names/aliases TSD
Species Homo sapiens
 Database cross references - HEXA
ExoCarta ExoCarta_3073
Vesiclepedia VP_3073
Entrez Gene 3073
HGNC 4878
MIM 606869
UniProt P06865  
 HEXA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HEXA
Molecular Function
    beta-N-acetylhexosaminidase activity GO:0004563 IBA
    beta-N-acetylhexosaminidase activity GO:0004563 IDA
    beta-N-acetylhexosaminidase activity GO:0004563 TAS
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 IDA
    beta-N-acetylglucosaminidase activity GO:0016231 IEA
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    skeletal system development GO:0001501 IEA
    carbohydrate metabolic process GO:0005975 IEA
    glycosaminoglycan biosynthetic process GO:0006024 IDA
    N-glycan processing GO:0006491 IBA
    ganglioside catabolic process GO:0006689 IBA
    ganglioside catabolic process GO:0006689 IDA
    lysosome organization GO:0007040 IEA
    sensory perception of sound GO:0007605 IEA
    adult walking behavior GO:0007628 IEA
    lipid storage GO:0019915 IEA
    sexual reproduction GO:0019953 IEA
    glycosaminoglycan metabolic process GO:0030203 IBA
    glycosaminoglycan metabolic process GO:0030203 IDA
    dermatan sulfate catabolic process GO:0030209 IEA
    hyaluronan catabolic process GO:0030214 IEA
    myelination GO:0042552 IEA
    cell morphogenesis involved in neuron differentiation GO:0048667 IEA
    neuromuscular process controlling posture GO:0050884 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    maintenance of location in cell GO:0051651 IEA
Subcellular Localization
    lysosome GO:0005764 IBA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    azurophil granule GO:0042582 IDA
    lysosomal lumen GO:0043202 NAS
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
    beta-N-acetylhexosaminidase complex GO:1905379 IDA
    beta-N-acetylhexosaminidase complex GO:1905379 IPI
 Experiment description of studies that identified HEXA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HEXA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 ARHGAP1 392
Co-fractionation Homo sapiens
3 LDHB 3945
Co-fractionation Homo sapiens
4 STIM2 57620
Co-fractionation Homo sapiens
5 YWHAE 7531
Co-fractionation Homo sapiens
6 ALDH2 217
Co-fractionation Homo sapiens
7 STK39 27347
Co-fractionation Homo sapiens
8 C9orf78 51759
Co-fractionation Homo sapiens
9 RNMT 8731
Co-fractionation Homo sapiens
10 ECH1 1891
Co-fractionation Homo sapiens
11 XRN2 22803
Co-fractionation Homo sapiens
12 APEX1 328
Affinity Capture-RNA Homo sapiens
13 PDCD10 11235
Co-fractionation Homo sapiens
14 GTF2H2C  
Co-fractionation Homo sapiens
15 TLN1 7094
Co-fractionation Homo sapiens
16 H2AFV 94239
Co-fractionation Homo sapiens
17 GDI1 2664
Co-fractionation Homo sapiens
18 HERC4 26091
Co-fractionation Homo sapiens
19 ADD1 118
Co-fractionation Homo sapiens
20 PLCG1 5335
Co-fractionation Homo sapiens
21 SEPT8 23176
Co-fractionation Homo sapiens
22 ATG16L1 55054
Co-fractionation Homo sapiens
23 HEXB 3074
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CCT7 10574
Co-fractionation Homo sapiens
25 HK1 3098
Co-fractionation Homo sapiens
26 RAD23A 5886
Co-fractionation Homo sapiens
27 KIF14 9928
Affinity Capture-MS Homo sapiens
28 PCNA 5111
Co-fractionation Homo sapiens
29 USP22 23326
Two-hybrid Homo sapiens
30 NSUN2 54888
Co-fractionation Homo sapiens
31 ACAT2 39
Co-fractionation Homo sapiens
32 TCP1 6950
Co-fractionation Homo sapiens
33 HMGCS1 3157
Co-fractionation Homo sapiens
34 MARS 4141
Co-fractionation Homo sapiens
35 SPARC 6678
Co-fractionation Homo sapiens
36 BAG6 7917
Affinity Capture-MS Homo sapiens
37 TRMT61A 115708
Co-fractionation Homo sapiens
38 WDR61 80349
Co-fractionation Homo sapiens
39 Ypel5  
Affinity Capture-MS Mus musculus
40 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
41 FH 2271
Co-fractionation Homo sapiens
42 MB21D1  
Proximity Label-MS Homo sapiens
43 HNRNPD 3184
Affinity Capture-MS Homo sapiens
44 AHCY 191
Co-fractionation Homo sapiens
45 YWHAZ 7534
Co-fractionation Homo sapiens
46 NUBP2 10101
Co-fractionation Homo sapiens
47 EFTUD1 79631
Co-fractionation Homo sapiens
48 PRKAR1A 5573
Co-fractionation Homo sapiens
49 CUL3 8452
Affinity Capture-MS Homo sapiens
50 UBR7  
Co-fractionation Homo sapiens
51 DHRS4 10901
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FBXO6 26270
Affinity Capture-MS Homo sapiens
53 GTF3C4 9329
Co-fractionation Homo sapiens
54 HIST1H2BO 8348
Co-fractionation Homo sapiens
55 SGTB  
Affinity Capture-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 HIBADH 11112
Co-fractionation Homo sapiens
58 PLA2G2D  
Affinity Capture-MS Homo sapiens
59 NUBP1 4682
Co-fractionation Homo sapiens
60 DNAJC3 5611
Co-fractionation Homo sapiens
61 KTN1 3895
Co-fractionation Homo sapiens
62 SEPT7 989
Co-fractionation Homo sapiens
63 ROCK1 6093
Co-fractionation Homo sapiens
64 H2AFX 3014
Affinity Capture-MS Homo sapiens
65 KRT18 3875
Co-fractionation Homo sapiens
66 TERF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CEP170P1  
Affinity Capture-MS Homo sapiens
68 SH2D3A  
Affinity Capture-MS Homo sapiens
69 LONP1 9361
Co-fractionation Homo sapiens
70 EZR 7430
Co-fractionation Homo sapiens
71 ALDH1B1 219
Co-fractionation Homo sapiens
72 FKBP9 11328
Co-fractionation Homo sapiens
73 CREBBP  
Two-hybrid Homo sapiens
74 CAPZB 832
Co-fractionation Homo sapiens
75 SSBP1 6742
Co-fractionation Homo sapiens
76 Tuba3a 22144
Affinity Capture-MS Mus musculus
77 ASMTL 8623
Co-fractionation Homo sapiens
78 GM2A 2760
Co-purification Homo sapiens
79 HNRNPH3 3189
Co-fractionation Homo sapiens
80 IGF2R 3482
Affinity Capture-MS Homo sapiens
81 RPRD1B 58490
Co-fractionation Homo sapiens
82 C1QBP 708
Co-fractionation Homo sapiens
83 UBA6 55236
Co-fractionation Homo sapiens
84 NUDT5 11164
Co-fractionation Homo sapiens
85 SULT2B1 6820
Co-fractionation Homo sapiens
86 XPNPEP1 7511
Co-fractionation Homo sapiens
View the network image/svg+xml



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