Gene description for DHRS4
Gene name dehydrogenase/reductase (SDR family) member 4
Gene symbol DHRS4
Other names/aliases CR
NRDR
PHCR
PSCD
SCAD-SRL
SDR-SRL
SDR25C1
SDR25C2
Species Homo sapiens
 Database cross references - DHRS4
ExoCarta ExoCarta_10901
Vesiclepedia VP_10901
Entrez Gene 10901
HGNC 16985
MIM 611596
UniProt Q9BTZ2  
 DHRS4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DHRS4
Molecular Function
    3-keto sterol reductase activity GO:0000253 IDA
    carbonyl reductase (NADPH) activity GO:0004090 IBA
    carbonyl reductase (NADPH) activity GO:0004090 IDA
    oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor GO:0016655 IDA
    alcohol dehydrogenase [NAD(P)+] activity GO:0018455 IDA
    3beta-hydroxy-5beta-steroid dehydrogenase activity GO:0033703 IEA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 TAS
Biological Process
    alcohol metabolic process GO:0006066 IDA
    steroid metabolic process GO:0008202 IDA
    ketone metabolic process GO:0042180 IDA
    retinol metabolic process GO:0042572 IEA
    retinal metabolic process GO:0042574 IBA
    positive regulation of reactive oxygen species metabolic process GO:2000379 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 ISS
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal matrix GO:0005782 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DHRS4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DHRS4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DMGDH  
Affinity Capture-MS Homo sapiens
2 PRSS16 10279
Affinity Capture-MS Homo sapiens
3 GSX1  
Affinity Capture-MS Homo sapiens
4 ECH1 1891
Affinity Capture-MS Homo sapiens
5 FOXA1  
Affinity Capture-MS Homo sapiens
6 PDE12 201626
Affinity Capture-MS Homo sapiens
7 CYP2B6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PMPCB 9512
Affinity Capture-MS Homo sapiens
9 HSD17B14  
Affinity Capture-MS Homo sapiens
10 WBSCR16  
Affinity Capture-MS Homo sapiens
11 DGKA 1606
Affinity Capture-MS Homo sapiens
12 GLRX5 51218
Affinity Capture-MS Homo sapiens
13 NDUFS3 4722
Affinity Capture-MS Homo sapiens
14 SLC25A3 5250
Affinity Capture-MS Homo sapiens
15 TP53 7157
Affinity Capture-MS Homo sapiens
16 PITRM1 10531
Affinity Capture-MS Homo sapiens
17 PCCB 5096
Affinity Capture-MS Homo sapiens
18 PHYHIPL  
Affinity Capture-MS Homo sapiens
19 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 EHHADH 1962
Affinity Capture-MS Homo sapiens
22 C17orf89  
Affinity Capture-MS Homo sapiens
23 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ALDH1B1 219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PDHA1 5160
Affinity Capture-MS Homo sapiens
26 AIFM2 84883
Affinity Capture-MS Homo sapiens
27 QPCT 25797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RICTOR 253260
Affinity Capture-MS Homo sapiens
29 MRPL37 51253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 COX6A1  
Affinity Capture-MS Homo sapiens
31 FAHD1 81889
Affinity Capture-MS Homo sapiens
32 BOLA1 51027
Affinity Capture-MS Homo sapiens
33 OXSM  
Co-fractionation Homo sapiens
34 MAGEA9  
Affinity Capture-MS Homo sapiens
35 NAT14 57106
Affinity Capture-MS Homo sapiens
36 NDUFS2 4720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 HEXA 3073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ESR1  
Affinity Capture-MS Homo sapiens
39 DUSP13  
Affinity Capture-MS Homo sapiens
40 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 NDUFAF3 25915
Affinity Capture-MS Homo sapiens
42 DHRS4L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CHCHD2  
Affinity Capture-MS Homo sapiens
45 NDUFAF5  
Affinity Capture-MS Homo sapiens
46 ZUFSP 221302
Affinity Capture-MS Homo sapiens
47 HHIPL1 84439
Affinity Capture-MS Homo sapiens
48 NRD1 4898
Affinity Capture-MS Homo sapiens
49 PRDX3 10935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MRPL13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PMPCA 23203
Affinity Capture-MS Homo sapiens
52 KLHL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CST11  
Affinity Capture-MS Homo sapiens
54 AQP3  
Affinity Capture-MS Homo sapiens
55 DHRS4 10901
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 POLDIP2 26073
Affinity Capture-MS Homo sapiens
57 PEX14 5195
Co-purification Homo sapiens
58 ACADVL 37
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 NDUFA5 4698
Affinity Capture-MS Homo sapiens
60 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
61 LY6G5C  
Affinity Capture-MS Homo sapiens
62 NDUFA10 4705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 BIRC7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 DHRS3 9249
Affinity Capture-MS Homo sapiens
65 LOC283788  
Affinity Capture-MS Homo sapiens
66 PRELP 5549
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 BCKDHA 593
Affinity Capture-MS Homo sapiens
68 AGPS 8540
Proximity Label-MS Homo sapiens
69 ABHD11 83451
Affinity Capture-MS Homo sapiens
70 TRIM43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TMEM70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CLPB 81570
Affinity Capture-MS Homo sapiens
73 SUN2 25777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 DKK2  
Affinity Capture-MS Homo sapiens
75 BDH2 56898
Affinity Capture-MS Homo sapiens
76 ATP5E 514
Affinity Capture-MS Homo sapiens
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