ExoCarta: Gene summary

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Gene description for ATP5E
Gene name	ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Gene symbol	ATP5E
Other names/aliases	ATPE MC5DN3
Species	Homo sapiens
Database cross references - ATP5E
ExoCarta	ExoCarta_514
Vesiclepedia	VP_514
Entrez Gene	514
HGNC	838
MIM	606153
UniProt	P56381
ATP5E identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells	26054723
Hepatocellular carcinoma cells	26054723

Gene ontology annotations for ATP5E

Molecular Function
	proton-transporting ATPase activity, rotational mechanism	GO:0046961	IEA
	transmembrane transporter activity	GO:0022857	IC
	proton-transporting ATP synthase activity, rotational mechanism	GO:0046933	IEA
	ATPase activity	GO:0016887	IDA
Biological Process
	small molecule metabolic process	GO:0044281	TAS
	respiratory electron transport chain	GO:0022904	TAS
	cellular metabolic process	GO:0044237	TAS
	mitochondrial ATP synthesis coupled proton transport	GO:0042776	TAS
Subcellular Localization
	mitochondrial proton-transporting ATP synthase complex	GO:0005753	IDA
	mitochondrial inner membrane	GO:0005743	IBA
	mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)	GO:0000275	IMP
	mitochondrial matrix	GO:0005759	TAS

Experiment description of studies that identified ATP5E in exosomes

Experiment ID

234

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocellular carcinoma cells

Sample name

HKCI-C3

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing