Gene description for ATP5E
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Gene symbol ATP5E
Other names/aliases ATPE
MC5DN3
Species Homo sapiens
 Database cross references - ATP5E
ExoCarta ExoCarta_514
Vesiclepedia VP_514
Entrez Gene 514
HGNC 838
MIM 606153
UniProt P56381  
 ATP5E identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ATP5E
Molecular Function
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IDA
Biological Process
    proton motive force-driven ATP synthesis GO:0015986 NAS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IBA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IDA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IC
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial matrix GO:0005759 TAS
    proton-transporting ATP synthase complex GO:0045259 IDA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IMP
 Experiment description of studies that identified ATP5E in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ATP5E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CISD3 284106
Affinity Capture-MS Homo sapiens
2 Anapc2  
Affinity Capture-MS Mus musculus
3 C15orf48  
Affinity Capture-MS Homo sapiens
4 ATP5J 522
Co-fractionation Homo sapiens
5 Prkci  
Affinity Capture-MS Mus musculus
6 CYP17A1 1586
Two-hybrid Homo sapiens
7 ATPIF1 93974
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
8 ADCK4 79934
Affinity Capture-MS Homo sapiens
9 Ruvbl1 56505
Affinity Capture-MS Mus musculus
10 ATP5I 521
Co-fractionation Homo sapiens
11 ATL3 25923
Affinity Capture-MS Homo sapiens
12 MCCC2 64087
Affinity Capture-MS Homo sapiens
13 TMEM63B 55362
Affinity Capture-MS Homo sapiens
14 COQ6 51004
Affinity Capture-MS Homo sapiens
15 NME4 4833
Affinity Capture-MS Homo sapiens
16 TRUB2  
Proximity Label-MS Homo sapiens
17 ATP5A1 498
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
18 C6orf203  
Affinity Capture-MS Homo sapiens
19 Hspa14 50497
Affinity Capture-MS Mus musculus
20 C17orf89  
Affinity Capture-MS Homo sapiens
21 COQ7  
Affinity Capture-MS Homo sapiens
22 EGFR 1956
Negative Genetic Homo sapiens
23 NDUFA4 4697
Affinity Capture-MS Homo sapiens
24 C21orf33  
Affinity Capture-MS Homo sapiens
25 SSBP1 6742
Proximity Label-MS Homo sapiens
26 OCIAD1 54940
Affinity Capture-MS Homo sapiens
27 BLK 640
Affinity Capture-MS Homo sapiens
28 C7orf55  
Affinity Capture-MS Homo sapiens
29 AGTRAP 57085
Two-hybrid Homo sapiens
30 Atp5a1 11946
Affinity Capture-MS Mus musculus
31 NDUFAF5  
Affinity Capture-MS Homo sapiens
32 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
33 COQ2  
Affinity Capture-MS Homo sapiens
34 USMG5 84833
Cross-Linking-MS (XL-MS) Homo sapiens
35 APOOL 139322
Affinity Capture-MS Homo sapiens
36 ATP5B 506
Co-fractionation Homo sapiens
37 COQ9  
Affinity Capture-MS Homo sapiens
38 CTDSPL2  
Affinity Capture-MS Homo sapiens
39 PTPMT1 114971
Affinity Capture-MS Homo sapiens
40 DHRS4 10901
Affinity Capture-MS Homo sapiens
41 HINT2 84681
Proximity Label-MS Homo sapiens
42 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
43 OXCT2  
Affinity Capture-MS Homo sapiens
44 UBE2N 7334
Affinity Capture-MS Homo sapiens
45 ATP5C1 509
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
46 Mad2l1 56150
Affinity Capture-MS Mus musculus
47 DDX27 55661
Cross-Linking-MS (XL-MS) Homo sapiens
48 SLC33A1 9197
Affinity Capture-MS Homo sapiens
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