Gene description for ATP5E
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Gene symbol ATP5E
Other names/aliases ATPE
MC5DN3
Species Homo sapiens
 Database cross references - ATP5E
ExoCarta ExoCarta_514
Entrez Gene 514
HGNC 838
MIM 606153
UniProt P56381  
 ATP5E identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ATP5E
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    transmembrane transporter activity GO:0022857 IC
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IEA
    ATPase activity GO:0016887 IDA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    respiratory electron transport chain GO:0022904 TAS
    cellular metabolic process GO:0044237 TAS
    mitochondrial ATP synthesis coupled proton transport GO:0042776 TAS
Subcellular Localization
    mitochondrial proton-transporting ATP synthase complex GO:0005753 IDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) GO:0000275 IMP
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ATP5E in exosomes
1
Experiment ID 234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ATP5E
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP5E is involved
PathwayEvidenceSource
Formation of ATP by chemiosmotic coupling TAS Reactome





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