Gene description for Hspa14
Gene name heat shock protein 14
Gene symbol Hspa14
Other names/aliases 70kDa
HSP70L1
Hsp70-4
NST-1
hsr.1
Species Mus musculus
 Database cross references - Hspa14
ExoCarta ExoCarta_50497
Vesiclepedia VP_50497
Entrez Gene 50497
UniProt Q99M31  
 Hspa14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspa14
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    heat shock protein binding GO:0031072 IBA
    protein folding chaperone GO:0044183 IBA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IEA
    protein refolding GO:0042026 IBA
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    ribosome GO:0005840 IBA
    ribosome GO:0005840 ISO
    ribosome GO:0005840 ISS
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified Hspa14 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspa14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC2 27000
Affinity Capture-MS Homo sapiens
2 AFG3L2 10939
Affinity Capture-MS Homo sapiens
3 GALNT2 2590
Affinity Capture-MS Homo sapiens
4 TDG 6996
Affinity Capture-MS Homo sapiens
5 DERL2 51009
Affinity Capture-MS Homo sapiens
6 ATP5E 514
Affinity Capture-MS Homo sapiens
7 SLC43A3 29015
Affinity Capture-MS Homo sapiens
8 DHCR7 1717
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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