Gene description for TMEM63B
Gene name transmembrane protein 63B
Gene symbol TMEM63B
Other names/aliases C6orf110
Species Homo sapiens
 Database cross references - TMEM63B
ExoCarta ExoCarta_55362
Vesiclepedia VP_55362
Entrez Gene 55362
HGNC 17735
UniProt Q5T3F8  
 TMEM63B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TMEM63B
Molecular Function
    calcium-activated cation channel activity GO:0005227 IBA
    calcium-activated cation channel activity GO:0005227 ISS
    mechanosensitive monoatomic ion channel activity GO:0008381 IDA
    mechanosensitive monoatomic ion channel activity GO:0008381 ISS
    mechanosensitive monoatomic cation channel activity GO:0140135 IDA
    osmolarity-sensing monoatomic cation channel activity GO:1990760 ISS
Biological Process
    exocytosis GO:0006887 IEA
    sensory perception of sound GO:0007605 ISS
    monoatomic cation transmembrane transport GO:0098655 IEA
    surfactant secretion GO:0160069 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    actin cytoskeleton GO:0015629 IDA
    early endosome membrane GO:0031901 IDA
    alveolar lamellar body membrane GO:0097233 IDA
 Experiment description of studies that identified TMEM63B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TMEM63B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
3 C5AR1  
Affinity Capture-MS Homo sapiens
4 CLCN7 1186
Affinity Capture-MS Homo sapiens
5 NKAIN1  
Affinity Capture-MS Homo sapiens
6 CHRNA4  
Affinity Capture-MS Homo sapiens
7 DERL2 51009
Affinity Capture-MS Homo sapiens
8 TSPAN17 26262
Affinity Capture-MS Homo sapiens
9 RXFP1  
Affinity Capture-MS Homo sapiens
10 SLC22A2 6582
Affinity Capture-MS Homo sapiens
11 OR10H2  
Affinity Capture-MS Homo sapiens
12 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TNPO1 3842
Affinity Capture-MS Homo sapiens
14 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SUB1 10923
Affinity Capture-MS Homo sapiens
16 TMEM9B 56674
Affinity Capture-MS Homo sapiens
17 PRPF40A 55660
Affinity Capture-MS Homo sapiens
18 HMMR  
Affinity Capture-MS Homo sapiens
19 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
20 MS4A15  
Affinity Capture-MS Homo sapiens
21 SLC38A5 92745
Affinity Capture-MS Homo sapiens
22 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
23 PCID2 55795
Affinity Capture-MS Homo sapiens
24 VKORC1 79001
Affinity Capture-MS Homo sapiens
25 MUC1 4582
Affinity Capture-MS Homo sapiens
26 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MAPK14 1432
Two-hybrid Homo sapiens
28 ATP2A2 488
Affinity Capture-MS Homo sapiens
29 PIGH  
Affinity Capture-MS Homo sapiens
30 MAGED2 10916
Affinity Capture-MS Homo sapiens
31 ATP5O 539
Affinity Capture-MS Homo sapiens
32 C3orf52  
Affinity Capture-MS Homo sapiens
33 C20orf24  
Affinity Capture-MS Homo sapiens
34 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
35 PHB 5245
Affinity Capture-MS Homo sapiens
36 GOLGA4  
Affinity Capture-MS Homo sapiens
37 F2RL1  
Affinity Capture-MS Homo sapiens
38 ATP5H 10476
Affinity Capture-MS Homo sapiens
39 LGALS9 3965
Affinity Capture-MS Homo sapiens
40 TMEM63C  
Affinity Capture-MS Homo sapiens
41 PABPN1 8106
Affinity Capture-MS Homo sapiens
42 OASL 8638
Affinity Capture-MS Homo sapiens
43 VAPA 9218
Affinity Capture-MS Homo sapiens
44 ENOPH1 58478
Affinity Capture-MS Homo sapiens
45 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
46 KCNK1  
Affinity Capture-MS Homo sapiens
47 MTUS2 23281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ATP5A1 498
Affinity Capture-MS Homo sapiens
49 SLC1A5 6510
Affinity Capture-MS Homo sapiens
50 RABL3 285282
Affinity Capture-MS Homo sapiens
51 EMD 2010
Affinity Capture-MS Homo sapiens
52 MS4A12  
Affinity Capture-MS Homo sapiens
53 ATP2B1 490
Affinity Capture-MS Homo sapiens
54 CHMP4B 128866
Affinity Capture-MS Homo sapiens
55 APOL2 23780
Affinity Capture-MS Homo sapiens
56 PCDHGC4  
Affinity Capture-MS Homo sapiens
57 SARAF  
Affinity Capture-MS Homo sapiens
58 SLC4A8 9498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 RPN1 6184
Affinity Capture-MS Homo sapiens
61 TMEM63A 9725
Affinity Capture-MS Homo sapiens
62 FLOT2 2319
Affinity Capture-MS Homo sapiens
63 SCN3B  
Affinity Capture-MS Homo sapiens
64 VDAC2 7417
Affinity Capture-MS Homo sapiens
65 ATP5B 506
Affinity Capture-MS Homo sapiens
66 TMEM185A  
Affinity Capture-MS Homo sapiens
67 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TMPO 7112
Affinity Capture-MS Homo sapiens
69 SLC30A2  
Affinity Capture-MS Homo sapiens
70 CXCR4 7852
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TTYH1  
Affinity Capture-MS Homo sapiens
72 AIFM1 9131
Affinity Capture-MS Homo sapiens
73 ATP5I 521
Affinity Capture-MS Homo sapiens
74 LMNB1 4001
Affinity Capture-MS Homo sapiens
75 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ZDHHC12  
Affinity Capture-MS Homo sapiens
77 TMEM109 79073
Affinity Capture-MS Homo sapiens
78 SRPRB 58477
Affinity Capture-MS Homo sapiens
79 CHRNA1  
Affinity Capture-MS Homo sapiens
80 SLC35E1 79939
Affinity Capture-MS Homo sapiens
81 SLC34A2 10568
Affinity Capture-MS Homo sapiens
82 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 YIPF5 81555
Affinity Capture-MS Homo sapiens
84 SGPP1 81537
Affinity Capture-MS Homo sapiens
85 ORMDL1 94101
Affinity Capture-MS Homo sapiens
86 SLC7A5 8140
Affinity Capture-MS Homo sapiens
87 GNA11 2767
Affinity Capture-MS Homo sapiens
88 TSPAN8 7103
Affinity Capture-MS Homo sapiens
89 YME1L1 10730
Affinity Capture-MS Homo sapiens
90 PCDH10 57575
Affinity Capture-MS Homo sapiens
91 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
92 ATP5E 514
Affinity Capture-MS Homo sapiens
93 TMCO1 54499
Affinity Capture-MS Homo sapiens
94 CMTM5  
Affinity Capture-MS Homo sapiens
95 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 YIPF4 84272
Affinity Capture-MS Homo sapiens
97 TGFA  
Affinity Capture-MS Homo sapiens
98 RAF1 5894
Affinity Capture-MS Homo sapiens
99 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 C9orf91 203197
Affinity Capture-MS Homo sapiens
101 ABCD3 5825
Affinity Capture-MS Homo sapiens
102 TAP1 6890
Affinity Capture-MS Homo sapiens
103 FLOT1 10211
Affinity Capture-MS Homo sapiens
104 SLC14A1  
Affinity Capture-MS Homo sapiens
105 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 SLC38A2 54407
Affinity Capture-MS Homo sapiens
107 SLC19A2 10560
Affinity Capture-MS Homo sapiens
108 PHB2 11331
Affinity Capture-MS Homo sapiens
109 IL17RB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 KCNA2  
Affinity Capture-MS Homo sapiens
111 SIDT2 51092
Affinity Capture-MS Homo sapiens
112 TP53 7157
Affinity Capture-MS Homo sapiens
113 GP9 2815
Affinity Capture-MS Homo sapiens
114 ERLIN1 10613
Affinity Capture-MS Homo sapiens
115 RBM25 58517
Affinity Capture-MS Homo sapiens
116 TMEM68  
Affinity Capture-MS Homo sapiens
117 ATP6V1A 523
Affinity Capture-MS Homo sapiens
118 LRRC55  
Affinity Capture-MS Homo sapiens
119 LGALS3 3958
Affinity Capture-MS Homo sapiens
120 TSPAN15 23555
Affinity Capture-MS Homo sapiens
121 ERLIN2 11160
Affinity Capture-MS Homo sapiens
122 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
123 SLC35F6 54978
Affinity Capture-MS Homo sapiens
124 SCAMP3 10067
Affinity Capture-MS Homo sapiens
125 Tmem63b  
Affinity Capture-MS Mus musculus
126 TLR9  
Affinity Capture-MS Homo sapiens
127 SCN4A  
Affinity Capture-MS Homo sapiens
128 DDX39A 10212
Affinity Capture-MS Homo sapiens
129 SOAT1 6646
Affinity Capture-MS Homo sapiens
130 SPPL2B 56928
Affinity Capture-MS Homo sapiens
131 ATP1A1 476
Affinity Capture-MS Homo sapiens
132 TUBD1  
Affinity Capture-MS Homo sapiens
133 LPAR2 9170
Affinity Capture-MS Homo sapiens
134 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 MARCH4  
Affinity Capture-MS Homo sapiens
136 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 CKAP4 10970
Affinity Capture-MS Homo sapiens
138 PTRF 284119
Affinity Capture-MS Homo sapiens
139 CAV1 857
Affinity Capture-MS Homo sapiens
140 FPR2  
Affinity Capture-MS Homo sapiens
141 MYOF 26509
Affinity Capture-MS Homo sapiens
142 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM63B is involved
No pathways found





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