Gene description for ATP5O
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Gene symbol ATP5O
Other names/aliases ATPO
OSCP
Species Homo sapiens
 Database cross references - ATP5O
ExoCarta ExoCarta_539
Entrez Gene 539
HGNC 850
MIM 600828
UniProt P48047  
 ATP5O identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ATP5O
Molecular Function
    ATPase activity GO:0016887 IDA
    transporter activity GO:0005215 NAS
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IEA
    drug binding GO:0008144 IDA
    protein binding GO:0005515 IPI
    transmembrane transporter activity GO:0022857 IC
Biological Process
    respiratory electron transport chain GO:0022904 TAS
    cellular metabolic process GO:0044237 TAS
    mitochondrial ATP synthesis coupled proton transport GO:0042776 TAS
    proton transport GO:0015992 NAS
    small molecule metabolic process GO:0044281 TAS
    ATP biosynthetic process GO:0006754 NAS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 IDA
    mitochondrial proton-transporting ATP synthase complex GO:0005753 NAS
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified ATP5O in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP5O
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP5O is involved
PathwayEvidenceSource
Formation of ATP by chemiosmotic coupling TAS Reactome





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