Gene description for DOCK8
Gene name dedicator of cytokinesis 8
Gene symbol DOCK8
Other names/aliases HEL-205
MRD2
ZIR8
Species Homo sapiens
 Database cross references - DOCK8
ExoCarta ExoCarta_81704
Vesiclepedia VP_81704
Entrez Gene 81704
HGNC 19191
MIM 611432
UniProt Q8NF50  
 DOCK8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DOCK8
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    immunological synapse formation GO:0001771 IEA
    small GTPase-mediated signal transduction GO:0007264 IEA
    regulation of Rho protein signal transduction GO:0035023 IBA
    dendritic cell migration GO:0036336 IEA
    positive regulation of GTPase activity GO:0043547 IDA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    memory T cell proliferation GO:0061485 IMP
    negative regulation of T cell apoptotic process GO:0070233 IEA
    positive regulation of establishment of T cell polarity GO:1903905 IBA
    positive regulation of establishment of T cell polarity GO:1903905 IMP
    cellular response to chemokine GO:1990869 IMP
    positive regulation of T cell migration GO:2000406 IBA
    positive regulation of T cell migration GO:2000406 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    cell leading edge GO:0031252 IBA
    leading edge membrane GO:0031256 IDA
    lamellipodium membrane GO:0031258 IEA
 Experiment description of studies that identified DOCK8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOCK8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Two-hybrid Homo sapiens
2 PDHA1 5160
Affinity Capture-MS Homo sapiens
3 LDHA 3939
Affinity Capture-MS Homo sapiens
4 MCM3 4172
Affinity Capture-MS Homo sapiens
5 ANP32A 8125
Affinity Capture-MS Homo sapiens
6 LDHB 3945
Affinity Capture-MS Homo sapiens
7 CPNE7 27132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PAICS 10606
Affinity Capture-MS Homo sapiens
9 BYSL 705
Two-hybrid Homo sapiens
10 SSB 6741
Affinity Capture-MS Homo sapiens
11 TMEM263 90488
Affinity Capture-MS Homo sapiens
12 ANXA6 309
Affinity Capture-MS Homo sapiens
13 SNRPF 6636
Affinity Capture-MS Homo sapiens
14 SRSF8 10929
Affinity Capture-MS Homo sapiens
15 RTP5  
Two-hybrid Homo sapiens
16 UBA1 7317
Affinity Capture-MS Homo sapiens
17 CKB 1152
Affinity Capture-MS Homo sapiens
18 RUVBL1 8607
Affinity Capture-MS Homo sapiens
19 SNRNP70 6625
Affinity Capture-MS Homo sapiens
20 PGK1 5230
Affinity Capture-MS Homo sapiens
21 PRDX3 10935
Affinity Capture-MS Homo sapiens
22 PFKFB2 5208
Affinity Capture-MS Homo sapiens
23 ABLIM1 3983
Affinity Capture-MS Homo sapiens
24 SHMT2 6472
Affinity Capture-MS Homo sapiens
25 KRT40  
Two-hybrid Homo sapiens
26 CALD1 800
Affinity Capture-MS Homo sapiens
27 MAGED2 10916
Affinity Capture-MS Homo sapiens
28 ATP5O 539
Affinity Capture-MS Homo sapiens
29 NME2 4831
Affinity Capture-MS Homo sapiens
30 APPBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PFN1 5216
Affinity Capture-MS Homo sapiens
32 BSG 682
Affinity Capture-MS Homo sapiens
33 AURKA 6790
Affinity Capture-MS Homo sapiens
34 DLD 1738
Affinity Capture-MS Homo sapiens
35 PPIB 5479
Affinity Capture-MS Homo sapiens
36 DSG1 1828
Affinity Capture-MS Homo sapiens
37 STIP1 10963
Affinity Capture-MS Homo sapiens
38 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
39 FAM124A  
Two-hybrid Homo sapiens
40 LRCH1 23143
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TRIM21 6737
Affinity Capture-MS Homo sapiens
42 TPI1 7167
Affinity Capture-MS Homo sapiens
43 S100A8 6279
Affinity Capture-MS Homo sapiens
44 EXOSC5 56915
Two-hybrid Homo sapiens
45 MEOX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 SMAD2 4087
Two-hybrid Homo sapiens
47 RPN1 6184
Affinity Capture-MS Homo sapiens
48 ZNF581  
Two-hybrid Homo sapiens
49 ZFC3H1 196441
Two-hybrid Homo sapiens
50 CRLF3 51379
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CEBPA  
Protein-peptide Homo sapiens
52 MRPL2 51069
Affinity Capture-MS Homo sapiens
53 LRCH3 84859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
54 BAG3 9531
Affinity Capture-MS Homo sapiens
55 ACAT1 38
Affinity Capture-MS Homo sapiens
56 IPO5 3843
Affinity Capture-MS Homo sapiens
57 MOB1B 92597
Proximity Label-MS Homo sapiens
58 RPN2 6185
Affinity Capture-MS Homo sapiens
59 DOCK8 81704
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 LRRC59 55379
Affinity Capture-MS Homo sapiens
61 TPR 7175
Affinity Capture-MS Homo sapiens
62 DOCK7 85440
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 LRCH4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 LMNB2 84823
Affinity Capture-MS Homo sapiens
65 S100A7 6278
Affinity Capture-MS Homo sapiens
66 SRSF5 6430
Affinity Capture-MS Homo sapiens
67 PDIA3 2923
Affinity Capture-MS Homo sapiens
68 LGALS7 3963
Affinity Capture-MS Homo sapiens
69 FBXL12 54850
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 CDC42 998
Two-hybrid Homo sapiens
71 MRPS28  
Affinity Capture-MS Homo sapiens
72 XPO1 7514
Affinity Capture-MS Homo sapiens
73 COG6 57511
Two-hybrid Homo sapiens
74 LUC7L2 51631
Affinity Capture-MS Homo sapiens
75 MTG1  
Two-hybrid Homo sapiens
76 MYO6 4646
Proximity Label-MS Homo sapiens
77 SF3A1 10291
Affinity Capture-MS Homo sapiens
78 DGCR6  
Two-hybrid Homo sapiens
79 HSPE1 3336
Affinity Capture-MS Homo sapiens
80 CARHSP1 23589
Two-hybrid Homo sapiens
81 SEPT7 989
Affinity Capture-MS Homo sapiens
82 LRCH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MRPL38  
Two-hybrid Homo sapiens
84 DOCK6 57572
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 NSMAF  
Affinity Capture-MS Homo sapiens
86 PA2G4 5036
Affinity Capture-MS Homo sapiens
87 ARPC3 10094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 CS 1431
Affinity Capture-MS Homo sapiens
89 RPS27A 6233
Affinity Capture-MS Homo sapiens
90 PRKCSH 5589
Affinity Capture-MS Homo sapiens
91 PCMT1 5110
Affinity Capture-MS Homo sapiens
92 FANCL 55120
Two-hybrid Homo sapiens
93 COX5A 9377
Affinity Capture-MS Homo sapiens
94 MOB1A 55233
Proximity Label-MS Homo sapiens
95 MORC3 23515
Affinity Capture-MS Homo sapiens
96 S100A9 6280
Affinity Capture-MS Homo sapiens
97 MED30  
Two-hybrid Homo sapiens
98 TAGLN2 8407
Affinity Capture-MS Homo sapiens
99 TGM3 7053
Affinity Capture-MS Homo sapiens
100 FAM161A  
Two-hybrid Homo sapiens
101 CLTA 1211
Affinity Capture-MS Homo sapiens
102 ANXA5 308
Affinity Capture-MS Homo sapiens
103 HBZ  
Two-hybrid Homo sapiens
104 RAC1 5879
Two-hybrid Homo sapiens
105 ANXA2 302
Affinity Capture-MS Homo sapiens
106 HSF2BP  
Two-hybrid Homo sapiens
107 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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