Gene description for DSG1
Gene name desmoglein 1
Gene symbol DSG1
Other names/aliases CDHF4
DG1
DSG
EPKHE
EPKHIA
PPKS1
SPPK1
Species Homo sapiens
 Database cross references - DSG1
ExoCarta ExoCarta_1828
Vesiclepedia VP_1828
Entrez Gene 1828
HGNC 3048
MIM 125670
UniProt Q02413  
 DSG1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DSG1
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    toxic substance binding GO:0015643 NAS
    gamma-catenin binding GO:0045295 IBA
    gamma-catenin binding GO:0045295 IPI
Biological Process
    cell-cell junction assembly GO:0007043 NAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 NAS
    response to progesterone GO:0032570 IEA
    protein stabilization GO:0050821 IDA
    maternal process involved in female pregnancy GO:0060135 IEA
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 NAS
Subcellular Localization
    cornified envelope GO:0001533 TAS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    apical plasma membrane GO:0016324 IEA
    lateral plasma membrane GO:0016328 IEA
    desmosome GO:0030057 IBA
    desmosome GO:0030057 IDA
    desmosome GO:0030057 NAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified DSG1 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DSG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 MAGEA3  
Affinity Capture-MS Homo sapiens
3 PKP2 5318
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 RPA2 6118
Proximity Label-MS Homo sapiens
5 SSB 6741
Affinity Capture-MS Homo sapiens
6 SAMD1  
Affinity Capture-MS Homo sapiens
7 SYDE2  
Affinity Capture-MS Homo sapiens
8 PI4KA 5297
Affinity Capture-MS Homo sapiens
9 S100A14 57402
Affinity Capture-MS Homo sapiens
10 KIF23 9493
Affinity Capture-MS Homo sapiens
11 MCM2 4171
Affinity Capture-MS Homo sapiens
12 IQGAP1 8826
Affinity Capture-MS Homo sapiens
13 COPS5 10987
Affinity Capture-MS Homo sapiens
14 DSC2 1824
Affinity Capture-Western Homo sapiens
15 Kalrn  
Affinity Capture-MS Rattus norvegicus
16 FLG 2312
Affinity Capture-MS Homo sapiens
17 KPRP 448834
Affinity Capture-MS Homo sapiens
18 CDC73  
Affinity Capture-MS Homo sapiens
19 MYCN  
Affinity Capture-MS Homo sapiens
20 USP53 54532
Affinity Capture-MS Homo sapiens
21 RLIM 51132
Affinity Capture-MS Homo sapiens
22 KIF14 9928
Affinity Capture-MS Homo sapiens
23 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 VAV1 7409
Affinity Capture-MS Homo sapiens
25 PARK2  
Affinity Capture-MS Homo sapiens
26 ZDHHC19  
Affinity Capture-MS Homo sapiens
27 RAD51  
Affinity Capture-MS Homo sapiens
28 ARG1 383
Affinity Capture-MS Homo sapiens
29 CCDC51 79714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TRIM21 6737
Affinity Capture-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 PLK1 5347
Affinity Capture-MS Homo sapiens
33 Arhgap18  
Affinity Capture-MS Mus musculus
34 CUL2 8453
Affinity Capture-MS Homo sapiens
35 CEP76  
Affinity Capture-MS Homo sapiens
36 DDX39B 7919
Affinity Capture-MS Homo sapiens
37 Wipi2 74781
Affinity Capture-MS Mus musculus
38 CYLD  
Affinity Capture-MS Homo sapiens
39 C1orf68 100129271
Affinity Capture-MS Homo sapiens
40 PMVK 10654
Affinity Capture-MS Homo sapiens
41 CASP14 23581
Affinity Capture-MS Homo sapiens
42 SLX4  
Affinity Capture-MS Homo sapiens
43 FANCD2  
Affinity Capture-MS Homo sapiens
44 METTL14  
Affinity Capture-MS Homo sapiens
45 DOCK8 81704
Affinity Capture-MS Homo sapiens
46 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 IQCB1  
Affinity Capture-MS Homo sapiens
48 HRNR 388697
Affinity Capture-MS Homo sapiens
49 GOT1 2805
Affinity Capture-MS Homo sapiens
50 CD274 29126
Affinity Capture-MS Homo sapiens
51 ZC3HC1  
Affinity Capture-MS Homo sapiens
52 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
53 CSTA 1475
Affinity Capture-MS Homo sapiens
54 SYDE1 85360
Affinity Capture-MS Homo sapiens
55 CDK2 1017
Affinity Capture-MS Homo sapiens
56 SOAT1 6646
Affinity Capture-MS Homo sapiens
57 PI4KAP1  
Affinity Capture-MS Homo sapiens
58 FBXO6 26270
Affinity Capture-MS Homo sapiens
59 FABP5 2171
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 BLMH 642
Affinity Capture-MS Homo sapiens
62 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
63 UBXN1 51035
Affinity Capture-MS Homo sapiens
64 CCDC8  
Affinity Capture-MS Homo sapiens
65 PKP3 11187
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
66 DSP 1832
Affinity Capture-MS Homo sapiens
67 PRSS16 10279
Affinity Capture-MS Homo sapiens
68 C21orf59 56683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ANXA2 302
Affinity Capture-MS Homo sapiens
70 PARP1 142
Affinity Capture-MS Homo sapiens
71 JUP 3728
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
72 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 TUBG1 7283
Affinity Capture-MS Homo sapiens
74 POLR2L 5441
Affinity Capture-MS Homo sapiens
75 MCM5 4174
Affinity Capture-MS Homo sapiens
76 USP1 7398
Affinity Capture-MS Homo sapiens
77 DSC1 1823
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
78 LINC00152  
Affinity Capture-RNA Homo sapiens
79 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
80 STK38L 23012
Affinity Capture-MS Homo sapiens
81 PKP1 5317
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
82 DCD 117159
Affinity Capture-MS Homo sapiens
83 KHDRBS2  
Affinity Capture-MS Homo sapiens
84 COQ2  
Affinity Capture-MS Homo sapiens
85 MAPK7  
Affinity Capture-MS Homo sapiens
86 TRIM37  
Proximity Label-MS Homo sapiens
87 OTUD4  
Affinity Capture-MS Homo sapiens
88 TGM3 7053
Affinity Capture-MS Homo sapiens
89 CDK4 1019
Affinity Capture-MS Homo sapiens
90 SERPINB12 89777
Affinity Capture-MS Homo sapiens
91 PINK1  
Affinity Capture-MS Homo sapiens
92 SSSCA1 10534
Affinity Capture-MS Homo sapiens
93 METTL3  
Affinity Capture-MS Homo sapiens
94 ZUFSP 221302
Affinity Capture-MS Homo sapiens
95 C9orf72  
Affinity Capture-MS Homo sapiens
96 OR2A4 79541
Affinity Capture-MS Homo sapiens
97 MYC  
Affinity Capture-MS Homo sapiens
98 ATXN3 4287
Affinity Capture-MS Homo sapiens
99 DDX3X 1654
Affinity Capture-MS Homo sapiens
100 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
101 PRMT1 3276
Affinity Capture-MS Homo sapiens
102 STX17 55014
Affinity Capture-MS Homo sapiens
103 FLG2 388698
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here