Gene description for CDK2
Gene name cyclin-dependent kinase 2
Gene symbol CDK2
Other names/aliases CDKN2
p33(CDK2)
Species Homo sapiens
 Database cross references - CDK2
ExoCarta ExoCarta_1017
Vesiclepedia VP_1017
Entrez Gene 1017
HGNC 1771
MIM 116953
UniProt P24941  
 CDK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for CDK2
Molecular Function
    histone kinase activity GO:0035173 IDA
    cyclin binding GO:0030332 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 TAS
    metal ion binding GO:0046872 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
Biological Process
    histone phosphorylation GO:0016572 IDA
    positive regulation of cell proliferation GO:0008284 IDA
    mitotic G1 DNA damage checkpoint GO:0031571 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    mitotic nuclear division GO:0007067 IEA
    centrosome duplication GO:0051298 TAS
    DNA repair GO:0006281 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    regulation of gene silencing GO:0060968 IDA
    cell division GO:0051301 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    cellular response to nitric oxide GO:0071732 TAS
    blood coagulation GO:0007596 TAS
    mitotic cell cycle GO:0000278 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    potassium ion transport GO:0006813 IEA
    meiotic nuclear division GO:0007126 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    DNA replication GO:0006260 TAS
    positive regulation of DNA-dependent DNA replication initiation GO:0032298 IEA
    Ras protein signal transduction GO:0007265 IEP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    endosome GO:0005768 IDA
    Y chromosome GO:0000806 IEA
    condensed chromosome GO:0000793 IEA
    cytosol GO:0005829 TAS
    X chromosome GO:0000805 IEA
    chromosome, telomeric region GO:0000781 IEA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    transcription factor complex GO:0005667 IEA
    centrosome GO:0005813 TAS
    Cajal body GO:0015030 IDA
 Experiment description of studies that identified CDK2 in exosomes
1
Experiment ID 489
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
EV Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
EV Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 254
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1|CD81
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 255
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 256
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 257
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 258
MISEV standards
EM
EV Biophysical techniques
TSG101|FLOT1|CD81
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 224
MISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101|CD63|CD81
EV Enriched markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
14
Experiment ID 191
MISEV standards
EV Biophysical techniques
Alix|CD81|CD9
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 217
MISEV standards
EM
EV Biophysical techniques
TSG101|CD81|CD9|CD63
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NAA25 80018
Affinity Capture-MS Homo sapiens
2 CNOT7 29883
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 CASP4  
Affinity Capture-MS Homo sapiens
4 CDC25A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 MCM3 4172
Affinity Capture-MS Homo sapiens
6 LYN 4067
Invivo Homo sapiens
Invitro Homo sapiens
7 RBL1  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
8 CCNA2 890
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
9 RRN3  
Reconstituted Complex Homo sapiens
10 ORC2L  
Affinity Capture-MS Homo sapiens
11 NPDC1  
Reconstituted Complex Homo sapiens
12 TP53 7157
Biochemical Activity Homo sapiens
13 PTMA 5757
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
14 FEN1 2237
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
15 RB1 5925
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
16 PPM1B 5495
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
17 SMAD3 4088
Affinity Capture-MS Homo sapiens
18 POLA  
Biochemical Activity Homo sapiens
19 E2F1 1869
Reconstituted Complex Homo sapiens
20 INCA1  
Biochemical Activity Homo sapiens
21 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 PCED1A  
Affinity Capture-MS Homo sapiens
23 CCNE2  
Invivo Homo sapiens
24 CDC6  
Affinity Capture-Western Homo sapiens
25 SKP2  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
26 FLJ10324  
Affinity Capture-MS Homo sapiens
27 CCNE1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 PRKCH 5583
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
29 VHL  
Affinity Capture-MS Homo sapiens
30 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
31 ALS2CR7  
Affinity Capture-MS Homo sapiens
32 CCNB3  
Reconstituted Complex Homo sapiens
33 CKS1B  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
34 SPDY1  
Affinity Capture-Western Homo sapiens
35 MS4A3  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
36 WRAP73 49856
Affinity Capture-MS Homo sapiens
37 PCNA 5111
Invitro Homo sapiens
38 UBE2M 9040
Affinity Capture-MS Homo sapiens
39 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RBM4 5936
Biochemical Activity Homo sapiens
41 PTP4A3  
Affinity Capture-MS Homo sapiens
42 CCNB1 891
Affinity Capture-Western Homo sapiens
43 TSC22D1 8848
Affinity Capture-MS Homo sapiens
44 OGT 8473
Affinity Capture-MS Homo sapiens
45 RBL2 5934
Invitro Homo sapiens
Biochemical Activity Homo sapiens
46 CDKN3 1033
Two-hybrid Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 CDK6 1021
Co-purification Homo sapiens
48 EPB41 2035
Affinity Capture-MS Homo sapiens
49 CDK2AP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 BCCIP 56647
Affinity Capture-Western Homo sapiens
51 PPP2CA 5515
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
52 XRCC6 2547
Biochemical Activity Homo sapiens
53 CCNA1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
54 FBL 2091
Affinity Capture-MS Homo sapiens
55 CHAF1B  
Affinity Capture-MS Homo sapiens
56 CDKN1B 1027
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 SORT1 6272
Affinity Capture-MS Homo sapiens
58 CEBPA  
Invitro Homo sapiens
Invivo Homo sapiens
59 NEDD8 4738
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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