Gene description for CDK2
Gene name cyclin-dependent kinase 2
Gene symbol CDK2
Other names/aliases CDKN2
p33(CDK2)
Species Homo sapiens
 Database cross references - CDK2
ExoCarta ExoCarta_1017
Entrez Gene 1017
HGNC 1771
MIM 116953
UniProt P24941  
 CDK2 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for CDK2
Molecular Function
    histone kinase activity GO:0035173 IDA
    cyclin binding GO:0030332 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 TAS
    metal ion binding GO:0046872 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
Biological Process
    histone phosphorylation GO:0016572 IDA
    positive regulation of cell proliferation GO:0008284 IDA
    mitotic G1 DNA damage checkpoint GO:0031571 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    mitotic nuclear division GO:0007067 IEA
    centrosome duplication GO:0051298 TAS
    DNA repair GO:0006281 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    regulation of gene silencing GO:0060968 IDA
    cell division GO:0051301 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    cellular response to nitric oxide GO:0071732 TAS
    blood coagulation GO:0007596 TAS
    mitotic cell cycle GO:0000278 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    potassium ion transport GO:0006813 IEA
    meiotic nuclear division GO:0007126 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    DNA replication GO:0006260 TAS
    positive regulation of DNA-dependent DNA replication initiation GO:0032298 IEA
    Ras protein signal transduction GO:0007265 IEP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    endosome GO:0005768 IDA
    Y chromosome GO:0000806 IEA
    condensed chromosome GO:0000793 IEA
    cytosol GO:0005829 TAS
    X chromosome GO:0000805 IEA
    chromosome, telomeric region GO:0000781 IEA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    transcription factor complex GO:0005667 IEA
    centrosome GO:0005813 TAS
    Cajal body GO:0015030 IDA
 Experiment description of studies that identified CDK2 in exosomes
1
Experiment ID 254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 CASP4  
Affinity Capture-MS Homo sapiens
3 CDC25A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 MCM3 4172
Affinity Capture-MS Homo sapiens
5 LYN 4067
Invivo Homo sapiens
Invitro Homo sapiens
6 WDR8  
Affinity Capture-MS Homo sapiens
7 CCNA2 890
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
8 RRN3  
Reconstituted Complex Homo sapiens
9 ORC2L  
Affinity Capture-MS Homo sapiens
10 NPDC1  
Reconstituted Complex Homo sapiens
11 PTMA 5757
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 FLJ10324  
Affinity Capture-MS Homo sapiens
13 FEN1 2237
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
14 RB1 5925
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
15 PPM1B 5495
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
16 TP53  
Biochemical Activity Homo sapiens
17 POLA  
Biochemical Activity Homo sapiens
18 E2F1 1869
Reconstituted Complex Homo sapiens
19 INCA1  
Biochemical Activity Homo sapiens
20 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 PCED1A  
Affinity Capture-MS Homo sapiens
22 CCNE2  
Invivo Homo sapiens
23 CDC6  
Affinity Capture-Western Homo sapiens
24 SKP2  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
25 EPB41 2035
Affinity Capture-MS Homo sapiens
26 CCNE1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 PRKCH 5583
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
28 VHL  
Affinity Capture-MS Homo sapiens
29 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
30 ALS2CR7  
Affinity Capture-MS Homo sapiens
31 CCNB3  
Reconstituted Complex Homo sapiens
32 CKS1B  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 SPDY1  
Affinity Capture-Western Homo sapiens
34 MS4A3  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
35 PCNA 5111
Invitro Homo sapiens
36 UBE2M 9040
Affinity Capture-MS Homo sapiens
37 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RBM4 5936
Biochemical Activity Homo sapiens
39 PTP4A3  
Affinity Capture-MS Homo sapiens
40 CCNB1 891
Affinity Capture-Western Homo sapiens
41 SMAD3  
Affinity Capture-MS Homo sapiens
42 OGT 8473
Affinity Capture-MS Homo sapiens
43 RBL2 5934
Invitro Homo sapiens
Biochemical Activity Homo sapiens
44 CDKN3 1033
Two-hybrid Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 CDK6 1021
Co-purification Homo sapiens
46 TSC22D1  
Affinity Capture-MS Homo sapiens
47 CDK2AP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 BCCIP 56647
Affinity Capture-Western Homo sapiens
49 PPP2CA 5515
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
50 XRCC6 2547
Biochemical Activity Homo sapiens
51 CCNA1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
52 RBL1  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
53 FBL 2091
Affinity Capture-MS Homo sapiens
54 CHAF1B  
Affinity Capture-MS Homo sapiens
55 CDKN1B 1027
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 FLJ13089  
Affinity Capture-MS Homo sapiens
57 SORT1 6272
Affinity Capture-MS Homo sapiens
58 CEBPA  
Invitro Homo sapiens
Invivo Homo sapiens
59 NEDD8 4738
Affinity Capture-MS Homo sapiens
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