Gene description for CTSA
Gene name cathepsin A
Gene symbol CTSA
Other names/aliases GLB2
GSL
NGBE
PPCA
PPGB
Species Homo sapiens
 Database cross references - CTSA
ExoCarta ExoCarta_5476
Entrez Gene 5476
HGNC 9251
MIM 613111
UniProt P10619  
 CTSA identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25857301    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CTSA
Molecular Function
    enzyme activator activity GO:0008047 TAS
    carboxypeptidase activity GO:0004180 TAS
    serine-type carboxypeptidase activity GO:0004185 IEA
Biological Process
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    small molecule metabolic process GO:0044281 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    post-translational protein modification GO:0043687 TAS
    proteolysis GO:0006508 IEA
    intracellular protein transport GO:0006886 TAS
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of catalytic activity GO:0043085 TAS
    sphingolipid metabolic process GO:0006665 TAS
    glycosphingolipid metabolic process GO:0006687 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleoplasm GO:0005654 IDA
    membrane GO:0016020 IDA
    endoplasmic reticulum GO:0005783 TAS
    lysosomal lumen GO:0043202 TAS
    lysosome GO:0005764 NAS
 Experiment description of studies that identified CTSA in exosomes
1
Experiment ID 250
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTSA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEU1 4758
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 CTNNBIP1 56998
Affinity Capture-MS Homo sapiens
3 CTSA 5476
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CTSA is involved
PathwayEvidenceSource
Glycosphingolipid metabolism TAS Reactome
MHC class II antigen presentation TAS Reactome
Sialic acid metabolism TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here