Gene ontology annotations for SGTA |
|
Experiment description of studies that identified SGTA in exosomes |
1 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
2 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
3 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
4 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
5 |
Experiment ID |
412 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
6 |
Experiment ID |
414 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
7 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
8 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
9 |
Experiment ID |
207 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
208 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
11 |
Experiment ID |
209 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
✔
VDAC
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
407 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
13 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
14 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
15 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
16 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
17 |
Experiment ID |
405 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Foreskin fibroblasts |
Sample name |
BJ |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
18 |
Experiment ID |
363 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
19 |
Experiment ID |
364 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
20 |
Experiment ID |
365 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
21 |
Experiment ID |
417 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Lymphoma cells |
Sample name |
Raji |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
22 |
Experiment ID |
411 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Mammary cancer-associated fibroblasts |
Sample name |
mCAF |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
23 |
Experiment ID |
418 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Monocytic leukemia cells |
Sample name |
THP-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
24 |
Experiment ID |
413 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Normal mammary epithelial cells |
Sample name |
MCF10A |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
25 |
Experiment ID |
211 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
cytochrome c|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
26 |
Experiment ID |
212 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
Cytochrome C|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
27 |
Experiment ID |
406 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
BxPC3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
28 |
Experiment ID |
415 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
29 |
Experiment ID |
434 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
30 |
Experiment ID |
435 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
31 |
Experiment ID |
408 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPDE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
32 |
Experiment ID |
409 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPNE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
33 |
Experiment ID |
416 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pluripotent stem cells |
Sample name |
PSC |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
34 |
Experiment ID |
191 |
MISEV standards |
✘
|
Biophysical techniques |
✔
Alix|CD81|CD9
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
35 |
Experiment ID |
410 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
T lymphocytes |
Sample name |
Jurkat |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
36 |
Experiment ID |
217 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|CD81|CD9|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for SGTA |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
1 |
HIST1H2BC |
8347 |
Cross-Linking-MS (XL-MS) |
|
Homo sapiens |
|
2 |
SGCA |
|
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
|
3 |
F11R |
50848 |
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
|
4 |
CTAG1A |
|
Two-hybrid |
|
Homo sapiens |
|
5 |
CDSN |
1041 |
Two-hybrid |
|
Homo sapiens |
|
6 |
ZG16 |
|
Two-hybrid |
|
Homo sapiens |
Two-hybrid |
|
Homo sapiens |
|
7 |
PPP1R3C |
|
Two-hybrid |
|
Homo sapiens |
|
8 |
PRB4 |
|
Two-hybrid |
|
Homo sapiens |
|
9 |
UBL4A |
8266 |
Affinity Capture-MS |
|
Homo sapiens |
Affinity Capture-MS |
|
Homo sapiens |
Affinity Capture-Western |
|
Homo sapiens |
Reconstituted Complex |
|
Homo sapiens |
Co-crystal Structure |
|
Homo sapiens |
Reconstituted Complex |
| | | |