Gene description for SRGN
Gene name serglycin
Gene symbol SRGN
Other names/aliases PPG
PRG
PRG1
Species Homo sapiens
 Database cross references - SRGN
ExoCarta ExoCarta_5552
Vesiclepedia VP_5552
Entrez Gene 5552
HGNC 9361
MIM 177040
UniProt P10124  
 SRGN identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 22723089    
 Gene ontology annotations for SRGN
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of cytokine production GO:0001818 ISS
    apoptotic process GO:0006915 IEA
    protein processing GO:0016485 ISS
    negative regulation of bone mineralization GO:0030502 IBA
    negative regulation of bone mineralization GO:0030502 IDA
    biomineral tissue development GO:0031214 IEA
    secretory granule organization GO:0033363 IBA
    mast cell secretory granule organization GO:0033364 ISS
    T cell secretory granule organization GO:0033371 ISS
    maintenance of protease location in mast cell secretory granule GO:0033373 ISS
    maintenance of granzyme B location in T cell secretory granule GO:0033382 ISS
    granzyme-mediated programmed cell death signaling pathway GO:0140507 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    Golgi apparatus GO:0005794 IDA
    secretory granule GO:0030141 IBA
    extracellular matrix GO:0031012 HDA
    platelet alpha granule lumen GO:0031093 TAS
    mast cell granule GO:0042629 ISS
    cytolytic granule GO:0044194 IEA
 Experiment description of studies that identified SRGN in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 136
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for SRGN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCALD 83988
Two-hybrid Homo sapiens
2 PEX12  
Two-hybrid Homo sapiens
3 UBR4 23352
Two-hybrid Homo sapiens
4 BAG6 7917
Two-hybrid Homo sapiens
5 GZMB  
Reconstituted Complex Homo sapiens
6 SGTB  
Two-hybrid Homo sapiens
7 SGTA 6449
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
9 UBQLN4 56893
Two-hybrid Homo sapiens
10 FHL3 2275
Two-hybrid Homo sapiens
11 UBQLN1 29979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 CEP70  
Two-hybrid Homo sapiens
13 PSRC1  
Two-hybrid Homo sapiens
14 UBQLN2 29978
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here