Gene description for SRGN
Gene name serglycin
Gene symbol SRGN
Other names/aliases PPG
PRG
PRG1
Species Homo sapiens
 Database cross references - SRGN
ExoCarta ExoCarta_5552
Vesiclepedia VP_5552
Entrez Gene 5552
HGNC 9361
MIM 177040
UniProt P10124  
 SRGN identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 22723089    
 Gene ontology annotations for SRGN
Molecular Function
    collagen binding GO:0005518 IEA
    protein binding GO:0005515 IPI
Biological Process
    platelet activation GO:0030168 TAS
    maintenance of protease location in mast cell secretory granule GO:0033373 ISS
    negative regulation of cytokine secretion GO:0050710 ISS
    mast cell secretory granule organization GO:0033364 ISS
    T cell secretory granule organization GO:0033371 ISS
    granzyme-mediated apoptotic signaling pathway GO:0008626 IDA
    biomineral tissue development GO:0031214 IEA
    protein processing GO:0016485 ISS
    blood coagulation GO:0007596 TAS
    negative regulation of bone mineralization GO:0030502 IDA
    platelet degranulation GO:0002576 TAS
    maintenance of granzyme B location in T cell secretory granule GO:0033382 ISS
Subcellular Localization
    platelet alpha granule lumen GO:0031093 TAS
    Golgi membrane GO:0000139 IEA
    extracellular space GO:0005615 IDA
    zymogen granule GO:0042588 IEA
    extracellular region GO:0005576 TAS
    Golgi apparatus GO:0005794 IDA
    mast cell granule GO:0042629 ISS
 Experiment description of studies that identified SRGN in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 136
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for SRGN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGTA 6449
Two-hybrid Homo sapiens
2 CD44 960
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCL3  
Invivo Homo sapiens
4 PF4 5196
Invivo Homo sapiens
Invitro Homo sapiens
5 GZMB  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SRGN is involved
PathwayEvidenceSource
Platelet degranulation TAS Reactome





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