Gene description for PHKA1
Gene name phosphorylase kinase, alpha 1 (muscle)
Gene symbol PHKA1
Other names/aliases PHKA
Species Homo sapiens
 Database cross references - PHKA1
ExoCarta ExoCarta_5255
Vesiclepedia VP_5255
Entrez Gene 5255
HGNC 8925
MIM 311870
UniProt P46020  
 PHKA1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PHKA1
Molecular Function
    phosphorylase kinase activity GO:0004689 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
Biological Process
    glycogen metabolic process GO:0005977 IEA
    generation of precursor metabolites and energy GO:0006091 TAS
    positive regulation of glycogen catabolic process GO:0045819 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    phosphorylase kinase complex GO:0005964 IBA
 Experiment description of studies that identified PHKA1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHKA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 CEP57  
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
5 SOD1 6647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 VAC14 55697
Co-fractionation Homo sapiens
7 KIAA1715 80856
Affinity Capture-MS Homo sapiens
8 PFN1 5216
Proximity Label-MS Homo sapiens
9 CALM2 805
Affinity Capture-MS Homo sapiens
10 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SAP18 10284
Affinity Capture-MS Homo sapiens
12 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FOXA1  
Affinity Capture-MS Homo sapiens
14 EYA2  
Affinity Capture-MS Homo sapiens
15 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CDKN1A  
Affinity Capture-MS Homo sapiens
17 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PHKA2 5256
Affinity Capture-MS Homo sapiens
19 GEMIN4 50628
Affinity Capture-MS Homo sapiens
20 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RDX 5962
Affinity Capture-MS Homo sapiens
22 PARD6B 84612
Affinity Capture-MS Homo sapiens
23 CALM3 808
Affinity Capture-MS Homo sapiens
24 MRPL55  
Co-fractionation Homo sapiens
25 SGTA 6449
Co-fractionation Homo sapiens
26 PTPRT  
Proximity Label-MS Homo sapiens
27 CUL4A 8451
Affinity Capture-MS Homo sapiens
28 TULP3 7289
Affinity Capture-MS Homo sapiens
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