Gene description for PARD6B
Gene name par-6 family cell polarity regulator beta
Gene symbol PARD6B
Other names/aliases PAR6B
Species Homo sapiens
 Database cross references - PARD6B
ExoCarta ExoCarta_84612
Vesiclepedia VP_84612
Entrez Gene 84612
HGNC 16245
MIM 608975
UniProt Q9BYG5  
 PARD6B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for PARD6B
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell-cell junction assembly GO:0007043 TAS
    centrosome cycle GO:0007098 IBA
    establishment or maintenance of cell polarity GO:0007163 IBA
    establishment or maintenance of cell polarity GO:0007163 TAS
    axonogenesis GO:0007409 TAS
    regulation of cell migration GO:0030334 TAS
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISS
    cell division GO:0051301 IEA
    regulation of cellular localization GO:0060341 IBA
    protein-containing complex assembly GO:0065003 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    bicellular tight junction GO:0005923 IDA
    cell cortex GO:0005938 IBA
    apical plasma membrane GO:0016324 IBA
    extracellular exosome GO:0070062 HDA
    tight junction GO:0070160 NAS
    PAR polarity complex GO:0120157 ISS
 Experiment description of studies that identified PARD6B in exosomes
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PARD6B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM3 4172
Co-fractionation Homo sapiens
2 RASSF8 11228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RHOQ 23433
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 IQGAP1 8826
Co-fractionation Homo sapiens
5 LLGL1 3996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KIAA2013 90231
Affinity Capture-MS Homo sapiens
7 PARD3 56288
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 EME1  
Affinity Capture-MS Homo sapiens
9 MAP4 4134
Co-fractionation Homo sapiens
10 ROBO1 6091
Affinity Capture-MS Homo sapiens
11 WDR83  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 PSMD10 5716
Co-fractionation Homo sapiens
13 LTN1 26046
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 CAPZB 832
Co-fractionation Homo sapiens
16 IARS2 55699
Affinity Capture-MS Homo sapiens
17 TRIM35 23087
Two-hybrid Homo sapiens
18 GNS 2799
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
19 KIF5B 3799
Co-fractionation Homo sapiens
20 AGGF1  
Affinity Capture-MS Homo sapiens
21 MAGOHB 55110
Two-hybrid Homo sapiens
22 ZSCAN4  
Two-hybrid Homo sapiens
23 PSMB6 5694
Co-fractionation Homo sapiens
24 RHOJ 57381
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 PTPN14 5784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PSMD5 5711
Affinity Capture-MS Homo sapiens
28 HSPA8 3312
Co-fractionation Homo sapiens
29 GOLIM4 27333
Affinity Capture-MS Homo sapiens
30 BANP  
Two-hybrid Homo sapiens
31 PPP1CA 5499
Affinity Capture-MS Homo sapiens
32 P4HA1 5033
Affinity Capture-MS Homo sapiens
33 AVL9 23080
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 DLGAP4 22839
Affinity Capture-MS Homo sapiens
36 PHKA1 5255
Affinity Capture-MS Homo sapiens
37 GOLGA2 2801
Two-hybrid Homo sapiens
38 PTPN21 11099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 YWHAE 7531
Affinity Capture-MS Homo sapiens
40 MYO10 4651
Affinity Capture-MS Homo sapiens
41 CRB3 92359
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 YWHAQ 10971
Affinity Capture-MS Homo sapiens
43 CEP76  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 AGPS 8540
Affinity Capture-MS Homo sapiens
45 ICAM5 7087
Affinity Capture-MS Homo sapiens
46 PDF  
Affinity Capture-MS Homo sapiens
47 ARL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 LRCH3 84859
Affinity Capture-MS Homo sapiens
49 RBM10  
Affinity Capture-MS Homo sapiens
50 UBR2 23304
Affinity Capture-MS Homo sapiens
51 RECQL5  
Affinity Capture-MS Homo sapiens
52 OGT 8473
Reconstituted Complex Homo sapiens
53 IPO5 3843
Co-fractionation Homo sapiens
54 KBTBD6  
Affinity Capture-MS Homo sapiens
55 YWHAZ 7534
Affinity Capture-MS Homo sapiens
56 CDCA3 83461
Affinity Capture-MS Homo sapiens
57 RNF170 81790
Affinity Capture-MS Homo sapiens
58 LAMP1 3916
Proximity Label-MS Homo sapiens
59 PRKCZ 5590
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ANKRD32  
Affinity Capture-MS Homo sapiens
61 SF1 7536
Affinity Capture-MS Homo sapiens
62 PTEN 5728
Negative Genetic Homo sapiens
63 ATAD3C 219293
Affinity Capture-MS Homo sapiens
64 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
65 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CDC42 998
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
67 PRKCI 5584
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
68 TP53BP2  
Affinity Capture-MS Homo sapiens
69 WWC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 EEFSEC 60678
Affinity Capture-MS Homo sapiens
71 INPP5B 3633
Affinity Capture-MS Homo sapiens
72 GMCL1  
Two-hybrid Homo sapiens
73 MSANTD4  
Affinity Capture-MS Homo sapiens
74 TMEM92  
Affinity Capture-MS Homo sapiens
75 FRMD6 122786
Two-hybrid Homo sapiens
76 IFT22 64792
Affinity Capture-MS Homo sapiens
77 PROSC 11212
Affinity Capture-MS Homo sapiens
78 DMBT1 1755
Affinity Capture-MS Homo sapiens
79 LLGL2 3993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 RASSF3 283349
Two-hybrid Homo sapiens
81 Prkci  
Affinity Capture-MS Mus musculus
82 CDCA4 55038
Two-hybrid Homo sapiens
83 EPB41L4B 54566
Affinity Capture-MS Homo sapiens
84 VPS28 51160
Affinity Capture-MS Homo sapiens
85 MKNK1  
Affinity Capture-MS Homo sapiens
86 PARD6G 84552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 HECTD3 79654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 CLPTM1 1209
Affinity Capture-MS Homo sapiens
89 ZZEF1 23140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 PDHA1 5160
Co-fractionation Homo sapiens
91 PBRM1 55193
Negative Genetic Homo sapiens
92 CCNK  
Affinity Capture-MS Homo sapiens
93 PSMA1 5682
Co-fractionation Homo sapiens
94 HNRNPL 3191
Co-fractionation Homo sapiens
95 ROBO2 6092
Affinity Capture-MS Homo sapiens
96 TRIB3  
Two-hybrid Homo sapiens
97 FOXM1  
Affinity Capture-MS Homo sapiens
98 EPHA2 1969
Proximity Label-MS Homo sapiens
99 KIFC1 3833
Affinity Capture-MS Homo sapiens
100 MRPL55  
Affinity Capture-MS Homo sapiens
101 RAC1 5879
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
102 HSF2BP  
Two-hybrid Homo sapiens
103 PARD6A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 DR1  
Affinity Capture-MS Homo sapiens
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