Gene description for PARD6B
Gene name par-6 family cell polarity regulator beta
Gene symbol PARD6B
Other names/aliases PAR6B
Species Homo sapiens
 Database cross references - PARD6B
ExoCarta ExoCarta_84612
Entrez Gene 84612
HGNC 16245
MIM 608975
UniProt Q9BYG5  
 PARD6B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for PARD6B
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell division GO:0051301 IEA
    protein complex assembly GO:0006461 IDA
    axonogenesis GO:0007409 TAS
    cell-cell junction organization GO:0045216 TAS
    bicellular tight junction assembly GO:0070830 TAS
    cell junction assembly GO:0034329 TAS
    cell-cell junction assembly GO:0007043 TAS
    establishment or maintenance of cell polarity GO:0007163 TAS
    regulation of cell migration GO:0030334 TAS
    cell cycle GO:0007049 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    apical part of cell GO:0045177 IEA
    plasma membrane GO:0005886 TAS
    bicellular tight junction GO:0005923 IDA
    nucleus GO:0005634 IEA
    cell cortex GO:0005938 IEA
    cytoplasm GO:0005737 IDA
    protein complex GO:0043234 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PARD6B in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PARD6B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARD3 56288
Invivo Homo sapiens
Invitro Homo sapiens
2 CDC42 998
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 RHOQ 23433
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PARD6B is involved
PathwayEvidenceSource
Tight junction interactions TAS Reactome





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