Gene description for EEFSEC
Gene name eukaryotic elongation factor, selenocysteine-tRNA-specific
Gene symbol EEFSEC
Other names/aliases EFSEC
SELB
Species Homo sapiens
 Database cross references - EEFSEC
ExoCarta ExoCarta_60678
Vesiclepedia VP_60678
Entrez Gene 60678
HGNC 24614
MIM 607695
UniProt P57772  
 EEFSEC identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EEFSEC
Molecular Function
    tRNA binding GO:0000049 IEA
    translation elongation factor activity GO:0003746 IBA
    GTPase activity GO:0003924 IDA
    GTP binding GO:0005525 IEA
    selenocysteine insertion sequence binding GO:0035368 IEA
    ribonucleoprotein complex binding GO:0043021 IEA
Biological Process
    selenocysteine incorporation GO:0001514 IBA
    selenocysteine incorporation GO:0001514 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified EEFSEC in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EEFSEC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Co-fractionation Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 Gspt1 14852
Affinity Capture-MS Mus musculus
5 NUDT21 11051
Co-fractionation Homo sapiens
6 GAN 8139
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 TERF2  
Affinity Capture-MS Homo sapiens
10 RPS3 6188
Co-fractionation Homo sapiens
11 POLR1B 84172
Co-fractionation Homo sapiens
12 PCF11 51585
Co-fractionation Homo sapiens
13 HSPA8 3312
Affinity Capture-MS Homo sapiens
14 RIT1 6016
Negative Genetic Homo sapiens
15 TFAP2A  
Co-fractionation Homo sapiens
16 RNF214  
Affinity Capture-MS Homo sapiens
17 CD6 923
Affinity Capture-MS Homo sapiens
18 PTEN 5728
Affinity Capture-MS Homo sapiens
19 SOX17  
Affinity Capture-MS Homo sapiens
20 RPL9 6133
Co-fractionation Homo sapiens
21 DDRGK1 65992
Affinity Capture-MS Homo sapiens
22 CLUH 23277
Co-fractionation Homo sapiens
23 POLR2A 5430
Co-fractionation Homo sapiens
24 NXF1 10482
Affinity Capture-RNA Homo sapiens
25 EFTUD2 9343
Co-fractionation Homo sapiens
26 RPS20 6224
Co-fractionation Homo sapiens
27 TRPT1 83707
Co-fractionation Homo sapiens
28 PARD6B 84612
Affinity Capture-MS Homo sapiens
29 Rrbp1  
Affinity Capture-MS Mus musculus
30 FBXW7  
Affinity Capture-MS Homo sapiens
31 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
32 HNRNPU 3192
Affinity Capture-MS Homo sapiens
33 Rpl35 66489
Affinity Capture-MS Mus musculus
34 PML 5371
Affinity Capture-MS Homo sapiens
35 CEBPA  
Protein-peptide Homo sapiens
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