Gene description for POLR2A
Gene name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Gene symbol POLR2A
Other names/aliases POLR2
POLRA
RPB1
RPBh1
RPO2
RPOL2
RpIILS
hRPB220
hsRPB1
Species Homo sapiens
 Database cross references - POLR2A
ExoCarta ExoCarta_5430
Vesiclepedia VP_5430
Entrez Gene 5430
HGNC 9187
MIM 180660
UniProt P24928  
 POLR2A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for POLR2A
Molecular Function
    DNA binding GO:0003677 NAS
    poly(A) RNA binding GO:0044822 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    metal ion binding GO:0046872 IEA
    DNA-directed RNA polymerase activity GO:0003899 NAS
    RNA-directed RNA polymerase activity GO:0003968 IEA
    RNA polymerase II activity GO:0001055 IBA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of viral transcription GO:0050434 TAS
    piRNA metabolic process GO:0034587 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    viral process GO:0016032 TAS
    somatic stem cell maintenance GO:0035019 TAS
    gene expression GO:0010467 TAS
    regulation of transcription, DNA-templated GO:0006355 NAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    transcription, RNA-templated GO:0001172 IEA
    nucleotide-excision repair GO:0006289 TAS
    DNA repair GO:0006281 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    positive regulation of RNA splicing GO:0033120 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 NAS
    nucleolus GO:0005730 IDA
    Prp19 complex GO:0000974 IDA
    DNA-directed RNA polymerase II, core complex GO:0005665 IDA
 Experiment description of studies that identified POLR2A in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2C 5432
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 PIN1 5300
Invitro Homo sapiens
Invitro Homo sapiens
3 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
4 MED19  
Affinity Capture-MS Homo sapiens
5 RDBP  
Affinity Capture-Western Homo sapiens
6 POLR2G 5436
Reconstituted Complex Homo sapiens
7 POLR2H 5437
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 POLR2F  
Reconstituted Complex Homo sapiens
9 SMARCB1 6598
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
10 SMARCA2 6595
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
11 CTDSP1 58190
Biochemical Activity Homo sapiens
12 TRAK1 22906
Invivo Homo sapiens
13 MDC1  
Protein-peptide Homo sapiens
14 CCNC  
Invivo Homo sapiens
Invitro Homo sapiens
15 CDK8 1024
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Co-fractionation Homo sapiens
16 PCIF1 63935
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 POLR2K  
Reconstituted Complex Homo sapiens
18 TCERG1 10915
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
19 NR0B2  
Reconstituted Complex Homo sapiens
20 PPIG 9360
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
21 HTATSF1 27336
Affinity Capture-Western Homo sapiens
22 POLR2E 5434
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
23 MED9  
Affinity Capture-MS Homo sapiens
24 TRG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 XAB2 56949
Affinity Capture-Western Homo sapiens
26 PCAF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 CTDP1 9150
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 CREBBP  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
29 POLR2L 5441
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
30 SUPT5H 6829
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 GTF2B 2959
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
32 NONO 4841
Invitro Homo sapiens
Invivo Homo sapiens
33 TBP  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
34 TCEA1 6917
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
35 SAFB 6294
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
36 CCNK  
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
37 ERCC6  
Invitro Homo sapiens
38 TAF11  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
39 CTDSP2 10106
Biochemical Activity Homo sapiens
40 CCNL2 81669
Affinity Capture-Western Homo sapiens
41 SMARCA4 6597
Invitro Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
42 MYC  
Reconstituted Complex Homo sapiens
43 FCP1  
Reconstituted Complex Homo sapiens
44 GTF2F2 2963
Invitro Homo sapiens
45 ZNF74  
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
46 CRSP7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 WHSC2  
Reconstituted Complex Homo sapiens
48 HNRNPF 3185
Invivo Homo sapiens
49 GTF2E1 2960
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
50 SMYD3 64754
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 EP300 2033
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
52 GTF2H4 2968
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
53 SURB7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
54 IXL  
Affinity Capture-MS Homo sapiens
55 SND1 27044
Invitro Homo sapiens
Invivo Homo sapiens
56 ELP3 55140
Co-purification Homo sapiens
57 CSH2  
Invivo Homo sapiens
Invitro Homo sapiens
58 BRCA1 672
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 MED28  
Affinity Capture-MS Homo sapiens
60 GTF2E2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which POLR2A is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Transcription Initiation IEA Reactome
MicroRNA (miRNA) biogenesis TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Pausing and recovery of HIV elongation TAS Reactome
Pausing and recovery of Tat-mediated HIV elongation TAS Reactome
PIWI-interacting RNA (piRNA) biogenesis TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Transcription of the HIV genome IEA Reactome
Transcription-coupled NER (TC-NER) TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of pluripotent stem cells TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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