Gene description for LIG3
Gene name ligase III, DNA, ATP-dependent
Gene symbol LIG3
Other names/aliases LIG2
Species Homo sapiens
 Database cross references - LIG3
ExoCarta ExoCarta_3980
Vesiclepedia VP_3980
Entrez Gene 3980
HGNC 6600
MIM 600940
UniProt P49916  
 LIG3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for LIG3
Molecular Function
    DNA binding GO:0003677 IEA
    DNA ligase activity GO:0003909 IDA
    DNA ligase activity GO:0003909 TAS
    DNA ligase (ATP) activity GO:0003910 IBA
    DNA ligase (ATP) activity GO:0003910 IDA
    DNA ligase (ATP) activity GO:0003910 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    double-strand break repair via homologous recombination GO:0000724 TAS
    DNA ligation GO:0006266 IDA
    lagging strand elongation GO:0006273 IBA
    base-excision repair, gap-filling GO:0006287 TAS
    base-excision repair, DNA ligation GO:0006288 IBA
    base-excision repair, DNA ligation GO:0006288 IDA
    base-excision repair, DNA ligation GO:0006288 IMP
    base-excision repair, DNA ligation GO:0006288 TAS
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 IDA
    mitochondrion organization GO:0007005 IDA
    V(D)J recombination GO:0033151 IDA
    mitochondrial DNA repair GO:0043504 IMP
    cell division GO:0051301 IEA
    DNA biosynthetic process GO:0071897 IEA
    negative regulation of mitochondrial DNA replication GO:0090298 IMP
    double-strand break repair via alternative nonhomologous end joining GO:0097681 IGI
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    DNA ligase III-XRCC1 complex GO:0070421 IDA
 Experiment description of studies that identified LIG3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 C6orf62 81688
Affinity Capture-MS Homo sapiens
3 MACROD1 28992
Affinity Capture-MS Homo sapiens
4 DNAJC8 22826
Cross-Linking-MS (XL-MS) Homo sapiens
5 JMJD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 THRB 7068
Affinity Capture-MS Homo sapiens
7 FOXD3  
Affinity Capture-MS Homo sapiens
8 RPA2 6118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NFATC1 4772
Affinity Capture-MS Homo sapiens
10 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
11 AIM2  
Affinity Capture-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
14 ST7  
Affinity Capture-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 VPS29 51699
Affinity Capture-MS Homo sapiens
18 CENPA  
Proximity Label-MS Homo sapiens
19 APEX1 328
Proximity Label-MS Homo sapiens
20 DDX23 9416
Proximity Label-MS Homo sapiens
21 WDR76  
Affinity Capture-MS Homo sapiens
22 PDXK 8566
Co-fractionation Homo sapiens
23 KIF23 9493
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
24 PRC1 9055
Affinity Capture-MS Homo sapiens
25 HMGA1 3159
Affinity Capture-MS Homo sapiens
26 HIST1H3A 8350
Proximity Label-MS Homo sapiens
27 ANKRD36 375248
Cross-Linking-MS (XL-MS) Homo sapiens
28 STIP1 10963
Cross-Linking-MS (XL-MS) Homo sapiens
29 HMGA2 8091
Affinity Capture-MS Homo sapiens
30 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
31 EED  
Affinity Capture-MS Homo sapiens
32 MECP2 4204
Affinity Capture-MS Homo sapiens
33 MYCN  
Affinity Capture-MS Homo sapiens
34 PREPL 9581
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CSNK2B 1460
Affinity Capture-MS Homo sapiens
36 TNFRSF1B  
Affinity Capture-MS Homo sapiens
37 KIF14 9928
Affinity Capture-MS Homo sapiens
38 Cebpb  
Affinity Capture-Western Rattus norvegicus
39 FOXP1 27086
Affinity Capture-MS Homo sapiens
40 PSME3 10197
Affinity Capture-MS Homo sapiens
41 H2AFZ 3015
Affinity Capture-MS Homo sapiens
42 NF2 4771
Affinity Capture-MS Homo sapiens
43 GATA4  
Affinity Capture-MS Homo sapiens
44 FOXC1  
Affinity Capture-MS Homo sapiens
45 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
46 PARK2  
Affinity Capture-MS Homo sapiens
47 APLF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PNKP 11284
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 BRD3 8019
Affinity Capture-MS Homo sapiens
50 RACGAP1 29127
Co-fractionation Homo sapiens
51 NBN 4683
Affinity Capture-MS Homo sapiens
52 HTT 3064
Affinity Capture-Western Homo sapiens
53 RNF146  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CBX3 11335
Proximity Label-MS Homo sapiens
55 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
56 BTF3 689
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 FOXK2  
Affinity Capture-MS Homo sapiens
59 RNF166  
Affinity Capture-MS Homo sapiens
60 TK2  
Affinity Capture-MS Homo sapiens
61 IFI16 3428
Affinity Capture-MS Homo sapiens
62 EXOSC4 54512
Affinity Capture-MS Homo sapiens
63 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
64 SRF  
Affinity Capture-MS Homo sapiens
65 RPA4  
Affinity Capture-MS Homo sapiens
66 HECTD1 25831
Affinity Capture-MS Homo sapiens
67 Kif20b  
Affinity Capture-MS Mus musculus
68 APTX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 POLB 5423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
70 NUDC 10726
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
71 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
72 PIPSL 266971
Affinity Capture-MS Homo sapiens
73 CD3EAP  
Proximity Label-MS Homo sapiens
74 CALML3 810
Affinity Capture-MS Homo sapiens
75 NUMA1 4926
Affinity Capture-MS Homo sapiens
76 TMPO 7112
Affinity Capture-MS Homo sapiens
77 HIST1H3F 8968
Affinity Capture-MS Homo sapiens
78 RPL31 6160
Proximity Label-MS Homo sapiens
79 BAX 581
Affinity Capture-MS Homo sapiens
80 TLX1  
Affinity Capture-MS Homo sapiens
81 CCT6A 908
Cross-Linking-MS (XL-MS) Homo sapiens
82 CTNNB1 1499
Affinity Capture-MS Homo sapiens
83 PTPRH 5794
Affinity Capture-MS Homo sapiens
84 RSAD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 SPC24 147841
Affinity Capture-MS Homo sapiens
86 NR3C1 2908
Proximity Label-MS Homo sapiens
87 TDP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TOP3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 H1F0 3005
Affinity Capture-MS Homo sapiens
90 EMX2  
Affinity Capture-MS Homo sapiens
91 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
92 NXF1 10482
Affinity Capture-RNA Homo sapiens
93 THRA  
Affinity Capture-MS Homo sapiens
94 JUN 3725
Affinity Capture-MS Homo sapiens
95 HIST1H3B 8358
Affinity Capture-MS Homo sapiens
96 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
97 HIST1H2BA 255626
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 EMC2 9694
Affinity Capture-MS Homo sapiens
99 TOP1 7150
Affinity Capture-MS Homo sapiens
100 BRD4 23476
Affinity Capture-MS Homo sapiens
101 PRKY  
Affinity Capture-MS Homo sapiens
102 NFATC2  
Affinity Capture-MS Homo sapiens
103 POLR2A 5430
Affinity Capture-Western Homo sapiens
104 HIST1H2BI 8346
Affinity Capture-MS Homo sapiens
105 SUPT16H 11198
Affinity Capture-MS Homo sapiens
106 PYHIN1  
Affinity Capture-MS Homo sapiens
107 XRCC5 7520
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
108 CCDC8  
Affinity Capture-MS Homo sapiens
109 NCAPG 64151
Affinity Capture-Western Homo sapiens
110 H2AFX 3014
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 FTL 2512
Affinity Capture-MS Homo sapiens
112 Cbx1  
Affinity Capture-MS Mus musculus
113 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
114 CLPX 10845
Affinity Capture-MS Homo sapiens
115 TP53 7157
Affinity Capture-MS Homo sapiens
116 MAX  
Affinity Capture-MS Homo sapiens
117 PYCARD 29108
Affinity Capture-MS Homo sapiens
118 SSRP1 6749
Affinity Capture-MS Homo sapiens
119 LARS2 23395
Affinity Capture-MS Homo sapiens
120 FOXB1  
Affinity Capture-MS Homo sapiens
121 C7orf55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 RPA1 6117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 MEN1 4221
Affinity Capture-MS Homo sapiens
124 HMGN5 79366
Affinity Capture-MS Homo sapiens
125 CCT8L2  
Affinity Capture-MS Homo sapiens
126 CMTR1 23070
Affinity Capture-MS Homo sapiens
127 H2AFB3  
Affinity Capture-MS Homo sapiens
128 XRCC1 7515
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
129 OGDHL  
Affinity Capture-MS Homo sapiens
130 PARP2  
Affinity Capture-MS Homo sapiens
131 MMGT1 93380
Affinity Capture-MS Homo sapiens
132 MALAT1 378938
Protein-RNA Homo sapiens
133 NEIL1  
Far Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
134 MOV10 4343
Affinity Capture-RNA Homo sapiens
135 RPS6 6194
Proximity Label-MS Homo sapiens
136 XRCC6 2547
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
137 ATXN3 4287
Affinity Capture-Western Homo sapiens
138 NUCKS1 64710
Affinity Capture-MS Homo sapiens
139 FOXE1  
Affinity Capture-MS Homo sapiens
140 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
142 NUCB1 4924
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here