Experiment description of studies that identified MALAT1 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

3 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

4 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

6 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

7 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for MALAT1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RUNX3   Affinity Capture-RNA Homo sapiens 2 FBXW7   Protein-RNA Homo sapiens 3 EHMT1   Co-localization Homo sapiens 4 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 5 HNRNPD 3184 Affinity Capture-RNA Homo sapiens 6 LIG3 3980 Protein-RNA Homo sapiens 7 PARP1 142 Affinity Capture-RNA Homo sapiens Protein-RNA Homo sapiens 8 KHSRP 8570 Affinity Capture-RNA Homo sapiens Protein-RNA Homo sapiens 9 ILF3 3609 Affinity Capture-RNA Homo sapiens Affinity Capture-RNA Homo sapiens 10 HMGB1 3146 Two-hybrid Homo sapiens 11 RPS6 6194 Affinity Capture-RNA Homo sapiens 12 AGO2 27161 Affinity Capture-RNA Homo sapiens View the network

Pathways in which MALAT1 is involved

No pathways found