Gene description for BAX
Gene name BCL2-associated X protein
Gene symbol BAX
Other names/aliases BCL2L4
Species Homo sapiens
 Database cross references - BAX
ExoCarta ExoCarta_581
Vesiclepedia VP_581
Entrez Gene 581
HGNC 959
MIM 600040
UniProt Q07812  
 BAX identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for BAX
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IDA
    protein homodimerization activity GO:0042803 IPI
    BH3 domain binding GO:0051434 IPI
    protein heterodimerization activity GO:0046982 IPI
    identical protein binding GO:0042802 IPI
    channel activity GO:0015267 IDA
Biological Process
    regulation of protein heterodimerization activity GO:0043497 IPI
    homeostasis of number of cells within a tissue GO:0048873 IEA
    regulation of protein homodimerization activity GO:0043496 IDA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    viral process GO:0016032 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IBA
    Sertoli cell proliferation GO:0060011 IEA
    regulation of cell cycle GO:0051726 IEA
    protein oligomerization GO:0051259 IDA
    development of secondary sexual characteristics GO:0045136 IEA
    cerebral cortex development GO:0021987 IEA
    thymocyte apoptotic process GO:0070242 IEA
    positive regulation of apoptotic process involved in mammary gland involution GO:0060058 IEA
    positive regulation of developmental pigmentation GO:0048087 IEA
    positive regulation of neuron apoptotic process GO:0043525 IDA
    retina development in camera-type eye GO:0060041 IEA
    response to axon injury GO:0048678 IEA
    T cell homeostatic proliferation GO:0001777 IEA
    response to salt stress GO:0009651 IEA
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IEA
    release of matrix enzymes from mitochondria GO:0032976 IDA
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IEA
    positive regulation of IRE1-mediated unfolded protein response GO:1903896 TAS
    regulation of mitochondrial membrane potential GO:0051881 IDA
    B cell homeostasis GO:0001782 IEA
    apoptotic mitochondrial changes GO:0008637 IDA
    apoptotic process involved in embryonic digit morphogenesis GO:1902263 IEA
    negative regulation of protein binding GO:0032091 IDA
    B cell receptor apoptotic signaling pathway GO:1990117 IDA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IDA
    fertilization GO:0009566 IEA
    endoplasmic reticulum calcium ion homeostasis GO:0032469 TAS
    positive regulation of apoptotic process GO:0043065 IMP
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IDA
    kidney development GO:0001822 IEA
    myeloid cell homeostasis GO:0002262 IEA
    response to gamma radiation GO:0010332 IEA
    B cell negative selection GO:0002352 IEA
    B cell homeostatic proliferation GO:0002358 IEA
    apoptotic process involved in patterning of blood vessels GO:1902262 IEA
    ovarian follicle development GO:0001541 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 IDA
    release of cytochrome c from mitochondria GO:0001836 IDA
    positive regulation of protein oligomerization GO:0032461 IDA
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    neuron apoptotic process GO:0051402 IEA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    protein homooligomerization GO:0051260 IDA
    retinal cell programmed cell death GO:0046666 IEA
    spermatid differentiation GO:0048515 IEA
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    positive regulation of endoplasmic reticulum unfolded protein response GO:1900103 IMP
    retinal cell apoptotic process GO:1990009 IMP
    positive regulation of apoptotic DNA fragmentation GO:1902512 IMP
    vagina development GO:0060068 IEA
    apoptotic signaling pathway GO:0097190 IDA
    blood vessel remodeling GO:0001974 IEA
    regulation of mitochondrial membrane permeability involved in programmed necrotic cell death GO:1902445 IEA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IC
    mitochondrion morphogenesis GO:0070584 IEA
    transformed cell apoptotic process GO:0006927 IMP
    hypothalamus development GO:0021854 IEA
    programmed cell death GO:0012501 TAS
    neuron migration GO:0001764 IEA
    response to toxic substance GO:0009636 IDA
    regulation of mammary gland epithelial cell proliferation GO:0033599 IEA
    negative regulation of fibroblast proliferation GO:0048147 IEA
    ectopic germ cell programmed cell death GO:0035234 IEA
    post-embryonic camera-type eye morphogenesis GO:0048597 IEA
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 TAS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IMP
    apoptotic process GO:0006915 TAS
    mitochondrial fusion GO:0008053 IDA
    regulation of nitrogen utilization GO:0006808 IEA
    positive regulation of B cell apoptotic process GO:0002904 IEA
    protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:0001844 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    glycosphingolipid metabolic process GO:0006687 IEA
    cellular response to UV GO:0034644 IEA
    B cell apoptotic process GO:0001783 IDA
    activation of signaling protein activity involved in unfolded protein response GO:0006987 TAS
    negative regulation of endoplasmic reticulum calcium ion concentration GO:0032471 IEA
    germ cell development GO:0007281 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IMP
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    mitochondrial permeability transition pore complex GO:0005757 IDA
    membrane GO:0016020 IDA
    Bcl-2 family protein complex GO:0097136 IDA
    mitochondrial outer membrane GO:0005741 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum GO:0005783 IDA
    mitochondrion GO:0005739 IDA
    BAX complex GO:0097144 IDA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IMP
    cytosol GO:0005829 TAS
    pore complex GO:0046930 IDA
 Experiment description of studies that identified BAX in exosomes
1
Experiment ID 79
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BAX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MOAP1  
Affinity Capture-MS Homo sapiens
2 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 MCL1 4170
Invitro Homo sapiens
4 OYE2  
Synthetic Rescue 4932
Phenotypic Enhancement 4932
Dosage Rescue 4932
5 BCL2L1 598
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 BAK1 578
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 VDAC1 7416
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 SNCA 6622
Reconstituted Complex Homo sapiens
9 HSPD1 3329
Affinity Capture-Western Homo sapiens
10 BCL2A1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 BCL2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 SLC25A4 291
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 PMAIP1  
Affinity Capture-Western Homo sapiens
14 SH3GLB1 51100
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 BAX 581
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 Bak1  
Affinity Capture-Western Rattus norvegicus
17 BCL2L10  
Affinity Capture-Western Homo sapiens
18 BNIPL  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BAX is involved
PathwayEvidenceSource
Activation, translocation and oligomerization of BAX TAS Reactome





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