Gene description for MAPK8
Gene name mitogen-activated protein kinase 8
Gene symbol MAPK8
Other names/aliases JNK
JNK-46
JNK1
JNK1A2
JNK21B1/2
PRKM8
SAPK1
SAPK1c
Species Homo sapiens
 Database cross references - MAPK8
ExoCarta ExoCarta_5599
Vesiclepedia VP_5599
Entrez Gene 5599
HGNC 6881
MIM 601158
UniProt P45983  
 MAPK8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MAPK8
Molecular Function
    enzyme binding GO:0019899 IPI
    JUN kinase activity GO:0004705 IDA
    histone deacetylase regulator activity GO:0035033 IMP
    histone deacetylase binding GO:0042826 IPI
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein binding GO:0005515 IPI
Biological Process
    stress-activated MAPK cascade GO:0051403 TAS
    positive regulation of determination of dorsal identity GO:2000017 IEA
    apoptotic process GO:0006915 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    response to stress GO:0006950 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    cellular response to lipopolysaccharide GO:0071222 IDA
    positive regulation of deacetylase activity GO:0090045 IMP
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    JUN phosphorylation GO:0007258 IDA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    negative regulation of apoptotic process GO:0043066 IDA
    innate immune response GO:0045087 TAS
    regulation of protein localization GO:0032880 IDA
    apoptotic signaling pathway GO:0097190 TAS
    response to UV GO:0009411 IDA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    ossification GO:0001503 IEA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    cellular response to nitric oxide GO:0071732 IEA
    positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:1900740 TAS
    programmed necrotic cell death GO:0097300 IEA
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 TAS
    cellular response to hydrogen peroxide GO:0070301 IEA
    peptidyl-threonine phosphorylation GO:0018107 IMP
    response to cadmium ion GO:0046686 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of protein metabolic process GO:0051247 IMP
    positive regulation of gene expression GO:0010628 IMP
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    programmed cell death GO:0012501 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    negative regulation of protein binding GO:0032091 IDA
    regulation of circadian rhythm GO:0042752 ISS
    positive regulation of cyclase activity GO:0031281 IMP
    rhythmic process GO:0048511 IEA
    regulation of histone deacetylation GO:0031063 IMP
    positive regulation of apoptotic process GO:0043065 TAS
    JNK cascade GO:0007254 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    protein phosphorylation GO:0006468 IMP
Subcellular Localization
    mitochondrion GO:0005739 IEA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified MAPK8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPK8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Invivo Homo sapiens
Invitro Homo sapiens
2 GSTP1 2950
Invivo Homo sapiens
Invitro Homo sapiens
3 DUSP22  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
4 IKBKAP 8518
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
5 SNCA 6622
Affinity Capture-Western Homo sapiens
6 CASP8 841
Co-purification Homo sapiens
7 FADD 8772
Co-purification Homo sapiens
8 DUSP1  
Two-hybrid Homo sapiens
9 SPIB  
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
10 MAP2K4 6416
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 DUSP10 11221
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 Rps6kb1  
Reconstituted Complex Mus musculus
13 HRAS 3265
Invivo Homo sapiens
14 TP53 7157
Invivo Homo sapiens
15 MAPK8IP2  
Invitro Homo sapiens
16 FAS 355
Co-purification Homo sapiens
17 SH2D3A  
Phenotypic Enhancement Homo sapiens
18 REL 5966
Invivo Homo sapiens
Two-hybrid Homo sapiens
19 TNFSF11  
Invivo Homo sapiens
20 EZR 7430
Co-purification Homo sapiens
21 SNCG 6623
Affinity Capture-Western Homo sapiens
22 FASLG 356
Co-purification Homo sapiens
23 PAX2  
Invivo Homo sapiens
Invitro Homo sapiens
24 JUND 3727
Reconstituted Complex Homo sapiens
25 BID  
Co-purification Homo sapiens
26 MAP3K2 10746
Invivo Homo sapiens
Invitro Homo sapiens
27 PRKD1 5587
Affinity Capture-Western Homo sapiens
28 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
29 MAP3K7  
Invivo Homo sapiens
Invitro Homo sapiens
30 BCAR1 9564
Affinity Capture-Western Homo sapiens
31 PRKDC 5591
Invivo Homo sapiens
Invitro Homo sapiens
32 ARHGDIA 396
Co-purification Homo sapiens
33 ATF2  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
34 JUN 3725
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
35 TNFRSF10B 8795
Co-purification Homo sapiens
36 SH3BP5 9467
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
37 MYC  
Invivo Homo sapiens
Invitro Homo sapiens
38 SPI1  
Biochemical Activity Homo sapiens
39 CRK 1398
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 CASP10  
Co-purification Homo sapiens
41 SPAG9 9043
Affinity Capture-Western Homo sapiens
42 JUNB  
Invivo Homo sapiens
Invitro Homo sapiens
43 XRCC6 2547
Invitro Homo sapiens
Invivo Homo sapiens
44 MAPK8IP3  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 MSN 4478
Co-purification Homo sapiens
46 DUSP16  
Invivo Homo sapiens
47 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
48 RHOA 387
Co-purification Homo sapiens
49 IRS1 3667
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 Dusp22  
Affinity Capture-Western Mus musculus
51 TNFRSF1A 7132
Co-purification Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here