Gene description for MAPK8IP1
Gene name mitogen-activated protein kinase 8 interacting protein 1
Gene symbol MAPK8IP1
Other names/aliases IB1
JIP-1
JIP1
PRKM8IP
Species Homo sapiens
 Database cross references - MAPK8IP1
ExoCarta ExoCarta_9479
Vesiclepedia VP_9479
Entrez Gene 9479
HGNC 6882
MIM 604641
UniProt Q9UQF2  
 MAPK8IP1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for MAPK8IP1
Molecular Function
    protein kinase inhibitor activity GO:0004860 TAS
    MAP-kinase scaffold activity GO:0005078 IBA
    MAP-kinase scaffold activity GO:0005078 IPI
    protein binding GO:0005515 IPI
    JUN kinase binding GO:0008432 IBA
    kinesin binding GO:0019894 IPI
Biological Process
    regulation of DNA-templated transcription GO:0006355 IEA
    JNK cascade GO:0007254 IBA
    vesicle-mediated transport GO:0016192 ISS
    negative regulation of JUN kinase activity GO:0043508 ISS
    regulation of JNK cascade GO:0046328 ISS
    positive regulation of JNK cascade GO:0046330 ISS
    regulation of CD8-positive, alpha-beta T cell proliferation GO:2000564 ISS
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    dendrite GO:0030425 IEA
    mitochondrial membrane GO:0031966 IEA
    neuronal cell body GO:0043025 IEA
    dentate gyrus mossy fiber GO:0044302 IEA
    synapse GO:0045202 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified MAPK8IP1 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for MAPK8IP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
2 SH3RF3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 RECQL5  
Two-hybrid Homo sapiens
4 SOCS3 9021
Affinity Capture-Western Homo sapiens
5 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
6 ERBB2 2064
Protein-peptide Homo sapiens
7 NEDD4 4734
Affinity Capture-MS Homo sapiens
8 MAPK8IP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MAPK8IP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SETX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KEAP1 9817
Affinity Capture-MS Homo sapiens
12 MYO18A 399687
Affinity Capture-MS Homo sapiens
13 TUBB8 347688
Affinity Capture-MS Homo sapiens
14 JUN 3725
Affinity Capture-Western Homo sapiens
15 PAX2  
Affinity Capture-Western Homo sapiens
16 TUBB3 10381
Affinity Capture-MS Homo sapiens
17 TAB1 10454
Co-fractionation Homo sapiens
18 MAP3K12  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 FEM1B 10116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 DUSP16  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 APP 351
Two-hybrid Homo sapiens
22 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 LRP1 4035
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
24 TTLL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MAP2K3 5606
Affinity Capture-Western Homo sapiens
26 MAPT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 MAP3K13 9175
Affinity Capture-Western Homo sapiens
28 HOXC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MAP3K7  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
30 MAPK9 5601
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 EGFR 1956
Protein-peptide Homo sapiens
32 LRP2 4036
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
33 MAP3K11 4296
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 ANK2 287
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 VRK2 7444
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 GNA13 10672
Affinity Capture-MS Homo sapiens
37 ANK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SENP5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
40 TIAM1  
Affinity Capture-Western Homo sapiens
41 MAPK8 5599
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MAP3K10  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 LRP8 7804
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MAPK8IP1 is involved
No pathways found





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