Gene description for LRP2
Gene name low density lipoprotein receptor-related protein 2
Gene symbol LRP2
Other names/aliases DBS
GP330
Species Homo sapiens
 Database cross references - LRP2
ExoCarta ExoCarta_4036
Vesiclepedia VP_4036
Entrez Gene 4036
HGNC 6694
MIM 600073
UniProt P98164  
 LRP2 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
Urine 25452312    
 Gene ontology annotations for LRP2
Molecular Function
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
    Wnt-activated receptor activity GO:0042813 IBA
    SH3 domain binding GO:0017124 IEA
    Wnt-protein binding GO:0017147 IBA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    forebrain development GO:0030900 IEA
    vitamin D metabolic process GO:0042359 TAS
    steroid metabolic process GO:0008202 TAS
    retinoid metabolic process GO:0001523 TAS
    lipid metabolic process GO:0006629 TAS
    protein glycosylation GO:0006486 TAS
    phototransduction, visible light GO:0007603 TAS
    endocytosis GO:0006897 TAS
    Wnt signaling pathway involved in dorsal/ventral axis specification GO:0044332 IBA
    canonical Wnt signaling pathway GO:0060070 IBA
    receptor-mediated endocytosis GO:0006898 IEA
    cell proliferation GO:0008283 IEA
Subcellular Localization
    endosome GO:0005768 IEA
    apical plasma membrane GO:0016324 ISS
    brush border membrane GO:0031526 IEA
    lysosomal membrane GO:0005765 IDA
    receptor complex GO:0043235 IDA
    lysosome GO:0005764 TAS
    Golgi apparatus GO:0005794 IEA
    coated pit GO:0005905 IEA
    endocytic vesicle GO:0030139 IEA
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum GO:0005783 IEA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified LRP2 in exosomes
1
Experiment ID 13
MISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
EV Biophysical techniques
AQP2
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
MISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70|CD9
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70|CD9
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 238
MISEV standards
EM
EV Biophysical techniques
TSG101
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LRP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANKS1B 56899
Two-hybrid Homo sapiens
2 APOB 338
Invitro Homo sapiens
Invivo Homo sapiens
3 APOH 350
Reconstituted Complex Homo sapiens
4 GIPC1 10755
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 ANKRA2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 DAB2 1601
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 SLC9A3 6550
Invivo Homo sapiens
Invitro Homo sapiens
8 MAPK8IP2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 DLG3 1741
Two-hybrid Homo sapiens
10 CUBN 8029
Invivo Homo sapiens
11 GC 2638
Affinity Capture-Western Homo sapiens
12 LPA 4018
Invitro Homo sapiens
13 ITGB1BP1 9270
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
14 DAB1 1600
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
15 DLG4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 CLU 1191
Invitro Homo sapiens
Invivo Homo sapiens
17 LRP2BP  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
18 INS 3630
Invitro Homo sapiens
19 SCN3A  
Two-hybrid Homo sapiens
20 MAPK8IP1 9479
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 LDLRAP1 26119
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
22 LRPAP1 4043
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
23 PLAU 5328
Reconstituted Complex Homo sapiens
24 SYNJ2BP 55333
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
25 APBA2 321
Two-hybrid Homo sapiens
26 SCGB1A1  
Reconstituted Complex Homo sapiens
27 TTR 7276
Invitro Homo sapiens
Invivo Homo sapiens
28 ANAPC10  
Two-hybrid Homo sapiens
29 ALB 213
Invivo Homo sapiens
30 CAPON  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
31 RBP1 5947
Reconstituted Complex Homo sapiens
32 ALB 280717
Reconstituted Complex Bos taurus
33 LPL 4023
Reconstituted Complex Homo sapiens
34 MAGI1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
35 DLG2 1740
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which LRP2 is involved
PathwayEvidenceSource
Retinoid metabolism and transport TAS Reactome
Vitamin D (calciferol) metabolism TAS Reactome





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