Gene description for LPL
Gene name lipoprotein lipase
Gene symbol LPL
Other names/aliases HDLCQ11
LIPD
Species Homo sapiens
 Database cross references - LPL
ExoCarta ExoCarta_4023
Vesiclepedia VP_4023
Entrez Gene 4023
HGNC 6677
MIM 609708
UniProt P06858  
 LPL identified in exosomes derived from the following tissue/cell type
Breast milk 17641064    
Breast milk 17641064    
Chondrocytes 35931686    
Chondrocytes 35931686    
Prostate cancer cells 25844599    
 Gene ontology annotations for LPL
Molecular Function
    triglyceride binding GO:0017129 IEA
    protein binding GO:0005515 IPI
    lipoprotein lipase activity GO:0004465 ISS
    phospholipase activity GO:0004620 ISS
    apolipoprotein binding GO:0034185 IPI
    triglyceride lipase activity GO:0004806 ISS
    heparin binding GO:0008201 IDA
    receptor binding GO:0005102 IPI
Biological Process
    triglyceride metabolic process GO:0006641 ISS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IC
    triglyceride biosynthetic process GO:0019432 IEA
    triglyceride catabolic process GO:0019433 ISS
    positive regulation of cholesterol storage GO:0010886 IMP
    phototransduction, visible light GO:0007603 TAS
    lipoprotein metabolic process GO:0042157 TAS
    response to drug GO:0042493 IEA
    retinoid metabolic process GO:0001523 TAS
    small molecule metabolic process GO:0044281 TAS
    very-low-density lipoprotein particle remodeling GO:0034372 ISS
    phospholipid metabolic process GO:0006644 ISS
    fatty acid biosynthetic process GO:0006633 ISS
    triglyceride homeostasis GO:0070328 IGI
    positive regulation of sequestering of triglyceride GO:0010890 IMP
    chylomicron remodeling GO:0034371 IC
    response to cold GO:0009409 IEA
Subcellular Localization
    extracellular matrix GO:0031012 IEA
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    very-low-density lipoprotein particle GO:0034361 IEA
    chylomicron GO:0042627 IEA
    plasma membrane GO:0005886 IEA
    anchored component of membrane GO:0031225 IEA
    cell surface GO:0009986 IEA
 Experiment description of studies that identified LPL in exosomes
1
Experiment ID 46
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 48
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
3
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for LPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2Z 65264
Two-hybrid Homo sapiens
2 PTPN4  
Two-hybrid Homo sapiens
3 COPS6 10980
Two-hybrid Homo sapiens
4 ASCC2  
Two-hybrid Homo sapiens
5 VLDLR 7436
Invitro Homo sapiens
6 LRP1 4035
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
7 LUC7L2 51631
Two-hybrid Homo sapiens
8 RPL18A 6142
Two-hybrid Homo sapiens
9 EMD  
Two-hybrid Homo sapiens
10 APOC2 344
Invitro Homo sapiens
11 KIAA1377  
Two-hybrid Homo sapiens
12 LRP2 4036
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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