Gene description for LRPAP1
Gene name low density lipoprotein receptor-related protein associated protein 1
Gene symbol LRPAP1
Other names/aliases A2MRAP
A2RAP
HBP44
MRAP
MYP23
RAP
alpha-2-MRAP
Species Homo sapiens
 Database cross references - LRPAP1
ExoCarta ExoCarta_4043
Vesiclepedia VP_4043
Entrez Gene 4043
HGNC 6701
MIM 104225
UniProt P30533  
 LRPAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LRPAP1
Molecular Function
    low-density lipoprotein particle receptor binding GO:0050750 IDA
    asialoglycoprotein receptor activity GO:0004873 TAS
    protein binding GO:0005515 IPI
    lipase binding GO:0035473 IEA
    receptor antagonist activity GO:0048019 IDA
    unfolded protein binding GO:0051082 TAS
    heparin binding GO:0008201 IEA
    very-low-density lipoprotein particle receptor binding GO:0070326 IPI
Biological Process
    receptor-mediated endocytosis GO:0006898 TAS
    negative regulation of very-low-density lipoprotein particle clearance GO:0010916 IDA
    negative regulation of protein binding GO:0032091 IDA
    vesicle-mediated transport GO:0016192 TAS
    extracellular negative regulation of signal transduction GO:1900116 IDA
    negative regulation of beta-amyloid clearance GO:1900222 IGI
    protein folding GO:0006457 TAS
Subcellular Localization
    extracellular region GO:0005576 IDA
    integral component of membrane GO:0016021 TAS
    plasma membrane GO:0005886 IDA
    rough endoplasmic reticulum lumen GO:0048237 IEA
    vesicle GO:0031982 IEA
    endoplasmic reticulum GO:0005783 IDA
    cell surface GO:0009986 IEA
 Experiment description of studies that identified LRPAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LRPAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LDLR 3949
Invivo Homo sapiens
Invitro Homo sapiens
2 RAF1 5894
Two-hybrid Homo sapiens
3 POLA2  
Two-hybrid Homo sapiens
4 VLDLR 7436
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 LRP1 4035
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
6 EIF4EBP1  
Affinity Capture-Western Homo sapiens
7 SORL1 6653
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
8 RTN4 57142
Two-hybrid Homo sapiens
9 RALGDS  
Two-hybrid Homo sapiens
10 SORT1 6272
Invitro Homo sapiens
11 MTOR 2475
Affinity Capture-Western Homo sapiens
12 LRP2 4036
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
13 RALA 5898
Reconstituted Complex Homo sapiens
14 LRP8 7804
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which LRPAP1 is involved
No pathways found





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