Gene description for BTN3A1
Gene name butyrophilin, subfamily 3, member A1
Gene symbol BTN3A1
Other names/aliases BT3.1
BTF5
BTN3.1
CD277
Species Homo sapiens
 Database cross references - BTN3A1
ExoCarta ExoCarta_11119
Vesiclepedia VP_11119
Entrez Gene 11119
HGNC 1138
MIM 613593
UniProt O00481  
 BTN3A1 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for BTN3A1
Molecular Function
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cytokine production GO:0001817 IBA
    positive regulation of cytokine production GO:0001819 IDA
    adaptive immune response GO:0002250 IEA
    positive regulation of type II interferon production GO:0032729 IMP
    activated T cell proliferation GO:0050798 IMP
    T cell receptor signaling pathway GO:0050852 IBA
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
 Experiment description of studies that identified BTN3A1 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BTN3A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 RABGEF1 27342
Affinity Capture-MS Homo sapiens
3 MTHFR 4524
Affinity Capture-MS Homo sapiens
4 GOLGA2 2801
Affinity Capture-MS Homo sapiens
5 CHAC2 494143
Affinity Capture-MS Homo sapiens
6 TRIM68 55128
Affinity Capture-MS Homo sapiens
7 BTN3A2 11118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PKMYT1  
Affinity Capture-MS Homo sapiens
9 CHRAC1  
Affinity Capture-MS Homo sapiens
10 ANKLE2 23141
Affinity Capture-MS Homo sapiens
11 CDKAL1  
Affinity Capture-MS Homo sapiens
12 MGC39584  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PIK3CB 5291
Affinity Capture-MS Homo sapiens
14 TAOK3 51347
Affinity Capture-MS Homo sapiens
15 TAOK1 57551
Affinity Capture-MS Homo sapiens
16 VEZT 55591
Affinity Capture-MS Homo sapiens
17 BTN3A3 10384
Affinity Capture-MS Homo sapiens
18 LRPAP1 4043
Affinity Capture-MS Homo sapiens
19 UACA 55075
Affinity Capture-MS Homo sapiens
20 PIK3CA 5290
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BTN3A1 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Butyrophilin (BTN) family interactions TAS Reactome
Immune System TAS Reactome





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