Gene description for ANKLE2
Gene name ankyrin repeat and LEM domain containing 2
Gene symbol ANKLE2
Other names/aliases KIAA0692
LEMD7
Lem4
Species Homo sapiens
 Database cross references - ANKLE2
ExoCarta ExoCarta_23141
Vesiclepedia VP_23141
Entrez Gene 23141
HGNC 29101
MIM 616062
UniProt Q86XL3  
 ANKLE2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ANKLE2
Molecular Function
    protein kinase inhibitor activity GO:0004860 IDA
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 TAS
    protein phosphatase 2A binding GO:0051721 IBA
    protein phosphatase 2A binding GO:0051721 IDA
Biological Process
    mitotic nuclear membrane reassembly GO:0007084 IBA
    mitotic nuclear membrane reassembly GO:0007084 IMP
    central nervous system development GO:0007417 IDA
    negative regulation of phosphorylation GO:0042326 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    regulation of catalytic activity GO:0050790 TAS
    cell division GO:0051301 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ANKLE2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ANKLE2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 OR2M7  
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 HSD17B11 51170
Proximity Label-MS Homo sapiens
5 KLK15  
Affinity Capture-MS Homo sapiens
6 MTHFR 4524
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 IMMP2L 83943
Affinity Capture-MS Homo sapiens
11 TCTN3  
Proximity Label-MS Homo sapiens
12 MAPK13 5603
Two-hybrid Homo sapiens
13 EMD 2010
Proximity Label-MS Homo sapiens
14 GJD3  
Proximity Label-MS Homo sapiens
15 LAMP2 3920
Proximity Label-MS Homo sapiens
16 DNAJC25 548645
Proximity Label-MS Homo sapiens
17 RET 5979
Proximity Label-MS Homo sapiens
18 PTPN1 5770
Proximity Label-MS Homo sapiens
19 ST7  
Proximity Label-MS Homo sapiens
20 TMPO 7112
Proximity Label-MS Homo sapiens
21 HSF2  
Affinity Capture-MS Homo sapiens
22 KIAA1429 25962
Affinity Capture-RNA Homo sapiens
23 PTRH2 51651
Proximity Label-MS Homo sapiens
24 B3GAT1  
Proximity Label-MS Homo sapiens
25 INSRR 3645
Affinity Capture-MS Homo sapiens
26 ABCA6 23460
Affinity Capture-MS Homo sapiens
27 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
28 OR6T1  
Affinity Capture-MS Homo sapiens
29 MPHOSPH8 54737
Affinity Capture-MS Homo sapiens
30 CCNF  
Proximity Label-MS Homo sapiens
31 RAB3B 5865
Proximity Label-MS Homo sapiens
32 FAM57A  
Affinity Capture-MS Homo sapiens
33 STX7 8417
Proximity Label-MS Homo sapiens
34 ZNF512B  
Two-hybrid Homo sapiens
35 SSR1 6745
Proximity Label-MS Homo sapiens
36 MARCH5  
Proximity Label-MS Homo sapiens
37 PIGH  
Affinity Capture-MS Homo sapiens
38 TMEM17  
Proximity Label-MS Homo sapiens
39 LMBR1L  
Affinity Capture-MS Homo sapiens
40 ATP2A3 489
Affinity Capture-MS Homo sapiens
41 CES1  
Cross-Linking-MS (XL-MS) Homo sapiens
42 DNAJC16  
Proximity Label-MS Homo sapiens
43 SYNE3 161176
Proximity Label-MS Homo sapiens
44 SLC25A46 91137
Proximity Label-MS Homo sapiens
45 IL17RA 23765
Affinity Capture-MS Homo sapiens
46 RAB35 11021
Proximity Label-MS Homo sapiens
47 TMEM106A  
Affinity Capture-MS Homo sapiens
48 NPAS1  
Affinity Capture-MS Homo sapiens
49 ASF1A 25842
Affinity Capture-MS Homo sapiens
50 ZFYVE9  
Two-hybrid Homo sapiens
51 RHOG 391
Affinity Capture-MS Homo sapiens
52 MARCKS 4082
Proximity Label-MS Homo sapiens
53 RHOH  
Affinity Capture-MS Homo sapiens
54 LMAN1 3998
Proximity Label-MS Homo sapiens
55 TMEM216  
Proximity Label-MS Homo sapiens
56 KIAA1715 80856
Proximity Label-MS Homo sapiens
57 PXMP2  
Proximity Label-MS Homo sapiens
58 EDC4 23644
Affinity Capture-MS Homo sapiens
59 RAB5A 5868
Proximity Label-MS Homo sapiens
60 FAM96A  
Affinity Capture-MS Homo sapiens
61 PANX1 24145
Proximity Label-MS Homo sapiens
62 PTPRN2  
Proximity Label-MS Homo sapiens
63 PLBD1 79887
Affinity Capture-MS Homo sapiens
64 SHISA3  
Affinity Capture-MS Homo sapiens
65 BCL2L11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SLC45A4  
Affinity Capture-MS Homo sapiens
67 PDGFRB 5159
Affinity Capture-MS Homo sapiens
68 CXADR 1525
Proximity Label-MS Homo sapiens
69 ZDHHC12  
Affinity Capture-MS Homo sapiens
70 RAB4A 5867
Proximity Label-MS Homo sapiens
71 HTT 3064
Affinity Capture-MS Homo sapiens
72 Immt  
Affinity Capture-MS Mus musculus
73 NUDT4 11163
Affinity Capture-MS Homo sapiens
74 RHOD 29984
Affinity Capture-MS Homo sapiens
75 SDF2L1 23753
Affinity Capture-MS Homo sapiens
76 VCP 7415
Affinity Capture-MS Homo sapiens
77 NTRK1 4914
Affinity Capture-MS Homo sapiens
78 OR2T33  
Affinity Capture-MS Homo sapiens
79 RFXANK  
Affinity Capture-MS Homo sapiens
80 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 KIF11 3832
Affinity Capture-MS Homo sapiens
82 DHFRL1  
Proximity Label-MS Homo sapiens
83 OSMR 9180
Affinity Capture-MS Homo sapiens
84 SIDT2 51092
Affinity Capture-MS Homo sapiens
85 ACAD11 84129
Affinity Capture-MS Homo sapiens
86 RHOT2 89941
Proximity Label-MS Homo sapiens
87 ELOVL5 60481
Proximity Label-MS Homo sapiens
88 SEC61B 10952
Proximity Label-MS Homo sapiens
89 UBXN6 80700
Affinity Capture-MS Homo sapiens
90 RMDN3 55177
Proximity Label-MS Homo sapiens
91 RTEL1 51750
Affinity Capture-MS Homo sapiens
92 TEX2 55852
Proximity Label-MS Homo sapiens
93 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
94 REEP5 7905
Proximity Label-MS Homo sapiens
95 CYP26B1  
Affinity Capture-MS Homo sapiens
96 SEC63 11231
Proximity Label-MS Homo sapiens
97 EVC2  
Proximity Label-MS Homo sapiens
98 RHBDD1 84236
Proximity Label-MS Homo sapiens
99 C4orf32  
Affinity Capture-MS Homo sapiens
100 SLC30A2  
Affinity Capture-MS Homo sapiens
101 RPN2 6185
Proximity Label-MS Homo sapiens
102 HTRA2 27429
Affinity Capture-MS Homo sapiens
103 CANX 821
Proximity Label-MS Homo sapiens
104 RAB7A 7879
Proximity Label-MS Homo sapiens
105 ZFPL1 7542
Proximity Label-MS Homo sapiens
106 LRRC59 55379
Proximity Label-MS Homo sapiens
107 GLMN 11146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 STAC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 STAC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 LAMP1 3916
Proximity Label-MS Homo sapiens
111 TMEM9B 56674
Affinity Capture-MS Homo sapiens
112 MEX3A  
Affinity Capture-RNA Homo sapiens
113 BCAP31 10134
Proximity Label-MS Homo sapiens
114 HSD3B7 80270
Proximity Label-MS Homo sapiens
115 STIM1 6786
Proximity Label-MS Homo sapiens
116 CHRNA7  
Affinity Capture-MS Homo sapiens
117 PIP 5304
Affinity Capture-MS Homo sapiens
118 FKBP8 23770
Proximity Label-MS Homo sapiens
119 METTL21B  
Affinity Capture-MS Homo sapiens
120 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
122 KIF26B  
Affinity Capture-MS Homo sapiens
123 CDC42 998
Affinity Capture-MS Homo sapiens
124 LEPR 3953
Affinity Capture-MS Homo sapiens
125 TRIM67  
Affinity Capture-MS Homo sapiens
126 ESYT2 57488
Proximity Label-MS Homo sapiens
127 RND2  
Affinity Capture-MS Homo sapiens
128 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 DNAI2  
Affinity Capture-MS Homo sapiens
130 ATP2A1 487
Proximity Label-MS Homo sapiens
131 EGFR 1956
Negative Genetic Homo sapiens
132 RAB9A 9367
Proximity Label-MS Homo sapiens
133 RPN1 6184
Proximity Label-MS Homo sapiens
134 PRKY  
Affinity Capture-MS Homo sapiens
135 STX4 6810
Proximity Label-MS Homo sapiens
136 CYP2C9  
Proximity Label-MS Homo sapiens
137 RAC2 5880
Affinity Capture-MS Homo sapiens
138 Dctn1 13191
Affinity Capture-MS Mus musculus
139 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 KCNA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 STX6 10228
Proximity Label-MS Homo sapiens
143 RNF43  
Proximity Label-MS Homo sapiens
144 RHOA 387
Affinity Capture-MS Homo sapiens
145 OR5L1  
Affinity Capture-MS Homo sapiens
146 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
147 IL17RB  
Affinity Capture-MS Homo sapiens
148 TMEM9 252839
Affinity Capture-MS Homo sapiens
149 LGI1  
Affinity Capture-MS Homo sapiens
150 STAC2  
Affinity Capture-MS Homo sapiens
151 UMODL1  
Affinity Capture-MS Homo sapiens
152 RHBDF2 79651
Proximity Label-MS Homo sapiens
153 CTXN1  
Affinity Capture-MS Homo sapiens
154 NUDT11 55190
Affinity Capture-MS Homo sapiens
155 RHOC 389
Affinity Capture-MS Homo sapiens
156 BTN3A1 11119
Affinity Capture-MS Homo sapiens
157 FCER1A  
Affinity Capture-MS Homo sapiens
158 OR1M1  
Affinity Capture-MS Homo sapiens
159 NUP155 9631
Proximity Label-MS Homo sapiens
160 ERGIC1 57222
Proximity Label-MS Homo sapiens
161 PDZD8 118987
Proximity Label-MS Homo sapiens
162 IL4R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 ERGIC2 51290
Proximity Label-MS Homo sapiens
164 TRIM37  
Proximity Label-MS Homo sapiens
165 BCL2  
Affinity Capture-MS Homo sapiens
166 SEC62 7095
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
167 FIS1 51024
Proximity Label-MS Homo sapiens
168 CEP135  
Proximity Label-MS Homo sapiens
169 RAB11A 8766
Proximity Label-MS Homo sapiens
170 RAC3 5881
Affinity Capture-MS Homo sapiens
171 CNGA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
172 EPHA2 1969
Proximity Label-MS Homo sapiens
173 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
174 DNAJC1 64215
Proximity Label-MS Homo sapiens
175 KRAS 3845
Negative Genetic Homo sapiens
176 CCDC47 57003
Affinity Capture-MS Homo sapiens
177 CKAP4 10970
Proximity Label-MS Homo sapiens
178 VIM 7431
Proximity Label-MS Homo sapiens
179 CCT8L2  
Affinity Capture-MS Homo sapiens
180 RPA3 6119
Proximity Label-MS Homo sapiens
181 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
182 ESYT1 23344
Proximity Label-MS Homo sapiens
183 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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