Gene description for ATP6V0A2
Gene name ATPase, H+ transporting, lysosomal V0 subunit a2
Gene symbol ATP6V0A2
Other names/aliases A2
ARCL
ARCL2A
ATP6A2
ATP6N1D
J6B7
RTF
STV1
TJ6
TJ6M
TJ6S
VPH1
WSS
Species Homo sapiens
 Database cross references - ATP6V0A2
ExoCarta ExoCarta_23545
Vesiclepedia VP_23545
Entrez Gene 23545
HGNC 18481
MIM 611716
UniProt Q9Y487  
 ATP6V0A2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ATP6V0A2
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ATPase binding GO:0051117 IBA
Biological Process
    intracellular iron ion homeostasis GO:0006879 IMP
    immune response GO:0006955 TAS
    vacuolar acidification GO:0007035 IBA
    regulation of macroautophagy GO:0016241 NAS
    cellular response to increased oxygen levels GO:0036295 IMP
    Golgi lumen acidification GO:0061795 NAS
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 IEA
    acrosomal vesicle GO:0001669 IEA
    lysosomal membrane GO:0005765 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IDA
    endosome membrane GO:0010008 TAS
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IBA
    phagocytic vesicle membrane GO:0030670 TAS
    proton-transporting V-type ATPase complex GO:0033176 NAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified ATP6V0A2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP6V0A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FAM131B  
Affinity Capture-MS Homo sapiens
3 ANKLE2 23141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RAB1A 5861
Affinity Capture-MS Homo sapiens
5 CD8B 926
Affinity Capture-MS Homo sapiens
6 KIAA2013 90231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BTF3 689
Affinity Capture-MS Homo sapiens
8 CD4 920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SLC2A1 6513
Affinity Capture-MS Homo sapiens
10 LGALS1 3956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMP2 3920
Affinity Capture-Western Homo sapiens
12 DNAJC25 548645
Proximity Label-MS Homo sapiens
13 TREM2  
Affinity Capture-MS Homo sapiens
14 ANXA6 309
Affinity Capture-MS Homo sapiens
15 SERINC2 347735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PTRH2 51651
Co-fractionation Homo sapiens
17 ERICH5  
Affinity Capture-MS Homo sapiens
18 COX11  
Affinity Capture-MS Homo sapiens
19 MUC1 4582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FBL 2091
Affinity Capture-MS Homo sapiens
21 OBSL1 23363
Affinity Capture-MS Homo sapiens
22 GJB3  
Affinity Capture-MS Homo sapiens
23 MTUS2 23281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ACP2 53
Affinity Capture-MS Homo sapiens
25 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CCDC115 84317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
30 RNF148  
Affinity Capture-MS Homo sapiens
31 STX12 23673
Affinity Capture-MS Homo sapiens
32 SLC25A3 5250
Co-fractionation Homo sapiens
33 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
34 RPL10L 140801
Co-fractionation Homo sapiens
35 TMEM63B 55362
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 RAB1B 81876
Affinity Capture-MS Homo sapiens
37 AQP1 358
Affinity Capture-MS Homo sapiens
38 POMGNT1 55624
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RNFT1  
Affinity Capture-MS Homo sapiens
40 CERS2 29956
Affinity Capture-MS Homo sapiens
41 RAB14 51552
Affinity Capture-MS Homo sapiens
42 MS4A12  
Affinity Capture-MS Homo sapiens
43 ATP6V1G2  
Affinity Capture-MS Homo sapiens
44 Rock1  
Affinity Capture-MS Mus musculus
45 EMP3 2014
Affinity Capture-MS Homo sapiens
46 GNAI1 2770
Affinity Capture-MS Homo sapiens
47 CLCN6 1185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MCAM 4162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
50 ATP6V0C 527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 DNAJC5 80331
Proximity Label-MS Homo sapiens
52 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CYS1 192668
Affinity Capture-MS Homo sapiens
54 ZNRF2 223082
Affinity Capture-Western Homo sapiens
55 TTC9C 283237
Affinity Capture-MS Homo sapiens
56 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 VMA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PPY  
Affinity Capture-MS Homo sapiens
59 SPRYD7 57213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 REEP5 7905
Affinity Capture-MS Homo sapiens
62 ANLN 54443
Affinity Capture-MS Homo sapiens
63 BCAM 4059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 VAMP3 9341
Affinity Capture-MS Homo sapiens
65 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CANX 821
Affinity Capture-MS Homo sapiens
67 RPS2 6187
Co-fractionation Homo sapiens
68 ATP5F1 515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SDHA 6389
Affinity Capture-MS Homo sapiens
70 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HOXB5  
Affinity Capture-MS Homo sapiens
72 RNF128  
Affinity Capture-MS Homo sapiens
73 SREBF1 6720
Negative Genetic Homo sapiens
74 ATP6V1D 51382
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 TMEM199  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SYT6  
Affinity Capture-MS Homo sapiens
78 SLC34A2 10568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 FTH1 2495
Affinity Capture-MS Homo sapiens
80 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
81 TCIRG1 10312
Affinity Capture-MS Homo sapiens
82 ATP6V0E1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PCDH10 57575
Affinity Capture-MS Homo sapiens
85 PPAPDC1A  
Affinity Capture-MS Homo sapiens
86 CERS6  
Affinity Capture-MS Homo sapiens
87 SLC12A6 9990
Affinity Capture-MS Homo sapiens
88 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 AKT1 207
Affinity Capture-MS Homo sapiens
91 C1orf95  
Affinity Capture-MS Homo sapiens
92 DNAJC6  
Affinity Capture-MS Homo sapiens
93 DRD2  
Two-hybrid Homo sapiens
94 SLC14A1  
Affinity Capture-MS Homo sapiens
95 SFT2D1 113402
Affinity Capture-MS Homo sapiens
96 EDIL3 10085
Affinity Capture-MS Homo sapiens
97 STK16 8576
Affinity Capture-MS Homo sapiens
98 ARRDC5  
Affinity Capture-MS Homo sapiens
99 KEAP1 9817
Negative Genetic Homo sapiens
100 TMCC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 EDEM1  
Affinity Capture-MS Homo sapiens
102 TYW1  
Affinity Capture-MS Homo sapiens
103 RPS15 6209
Co-fractionation Homo sapiens
104 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
105 GPRC5D  
Affinity Capture-MS Homo sapiens
106 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SFTPC  
Affinity Capture-MS Homo sapiens
108 XKR4  
Affinity Capture-MS Homo sapiens
109 C10orf88  
Affinity Capture-MS Homo sapiens
110 ATP6AP1 537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 TUBD1  
Affinity Capture-MS Homo sapiens
113 AQP5 362
Affinity Capture-MS Homo sapiens
114 ATP11C 286410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 CAV1 857
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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