Gene description for PTRH2
Gene name peptidyl-tRNA hydrolase 2
Gene symbol PTRH2
Other names/aliases 2
BIT1
CFAP37
CGI-147
IMNEPD
PTH
PTH 2
PTH2
Species Homo sapiens
 Database cross references - PTRH2
ExoCarta ExoCarta_51651
Vesiclepedia VP_51651
Entrez Gene 51651
HGNC 24265
MIM 608625
UniProt Q9Y3E5  
 PTRH2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PTRH2
Molecular Function
    aminoacyl-tRNA hydrolase activity GO:0004045 IBA
    aminoacyl-tRNA hydrolase activity GO:0004045 IMP
    protein binding GO:0005515 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of anoikis GO:2000210 IBA
    positive regulation of anoikis GO:2000210 IMP
    negative regulation of anoikis GO:2000811 IBA
    negative regulation of anoikis GO:2000811 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrial outer membrane GO:0005741 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IMP
    membrane GO:0016020 HDA
 Experiment description of studies that identified PTRH2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTRH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
2 TM4SF5  
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 SLC31A2  
Affinity Capture-MS Homo sapiens
5 OR10H1  
Affinity Capture-MS Homo sapiens
6 OR10H2  
Affinity Capture-MS Homo sapiens
7 SYNGR1 9145
Affinity Capture-MS Homo sapiens
8 AES 166
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 SQSTM1 8878
Proximity Label-MS Homo sapiens
10 DNAJC25 548645
Proximity Label-MS Homo sapiens
11 PTPN1 5770
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
12 FFAR1  
Affinity Capture-MS Homo sapiens
13 PDE2A  
Affinity Capture-MS Homo sapiens
14 SERINC2 347735
Affinity Capture-MS Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 ISM2  
Affinity Capture-MS Homo sapiens
17 PHYHIP 9796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 COQ9  
Affinity Capture-MS Homo sapiens
19 CHCHD2  
Affinity Capture-MS Homo sapiens
20 PRC1 9055
Affinity Capture-MS Homo sapiens
21 YIPF3 25844
Affinity Capture-MS Homo sapiens
22 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LAMTOR3 8649
Co-fractionation Homo sapiens
24 CCDC47 57003
Affinity Capture-MS Homo sapiens
25 GRPR  
Affinity Capture-MS Homo sapiens
26 ATP6V0A1 535
Co-fractionation Homo sapiens
27 ABCC2 1244
Co-fractionation Homo sapiens
28 SLC6A15 55117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 UFL1 23376
Affinity Capture-MS Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 RB1CC1 9821
Affinity Capture-MS Homo sapiens
32 CD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 DUSP8  
Affinity Capture-MS Homo sapiens
34 CBX3 11335
Proximity Label-MS Homo sapiens
35 CDKAL1  
Affinity Capture-MS Homo sapiens
36 EYA3  
Affinity Capture-MS Homo sapiens
37 ACTB 60
Proximity Label-MS Homo sapiens
38 TEX29  
Affinity Capture-MS Homo sapiens
39 FLOT2 2319
Co-fractionation Homo sapiens
40 SEC61B 10952
Affinity Capture-MS Homo sapiens
41 DUSP22  
Affinity Capture-MS Homo sapiens
42 CLEC5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GPIHBP1  
Affinity Capture-MS Homo sapiens
45 CXCR4 7852
Affinity Capture-MS Homo sapiens
46 CANX 821
Affinity Capture-MS Homo sapiens
47 RPS2 6187
Co-fractionation Homo sapiens
48 RAB5A 5868
Proximity Label-MS Homo sapiens
49 CHST8 64377
Affinity Capture-MS Homo sapiens
50 HMOX2 3163
Affinity Capture-MS Homo sapiens
51 SIGLEC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MEX3A  
Affinity Capture-RNA Homo sapiens
53 ATP6V1D 51382
Co-fractionation Homo sapiens
54 SYT6  
Affinity Capture-MS Homo sapiens
55 NIPAL1  
Affinity Capture-MS Homo sapiens
56 GADD45GIP1  
Co-fractionation Homo sapiens
57 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TMEM169  
Affinity Capture-MS Homo sapiens
60 DUSP6 1848
Affinity Capture-MS Homo sapiens
61 SAAL1 113174
Affinity Capture-MS Homo sapiens
62 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 SCGB2A2  
Affinity Capture-MS Homo sapiens
65 GPR21  
Affinity Capture-MS Homo sapiens
66 RPL35A 6165
Affinity Capture-MS Homo sapiens
67 STT3B 201595
Co-fractionation Homo sapiens
68 TGFA  
Affinity Capture-MS Homo sapiens
69 DUSP9  
Affinity Capture-MS Homo sapiens
70 DDRGK1 65992
Affinity Capture-MS Homo sapiens
71 CLTC 1213
Affinity Capture-MS Homo sapiens
72 CD55 1604
Co-fractionation Homo sapiens
73 SLC22A4 6583
Affinity Capture-MS Homo sapiens
74 AKAP1 8165
Proximity Label-MS Homo sapiens
75 FLOT1 10211
Co-fractionation Homo sapiens
76 LHFP 10186
Affinity Capture-MS Homo sapiens
77 MRPL42  
Affinity Capture-MS Homo sapiens
78 FASN 2194
Positive Genetic Homo sapiens
79 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
80 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 JUP 3728
Co-fractionation Homo sapiens
82 FBXL6  
Affinity Capture-MS Homo sapiens
83 RTN3 10313
Affinity Capture-MS Homo sapiens
84 TLR5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 RPS15 6209
Co-fractionation Homo sapiens
86 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 WIF1 11197
Affinity Capture-MS Homo sapiens
88 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 MED21  
Affinity Capture-MS Homo sapiens
91 TECTB  
Affinity Capture-MS Homo sapiens
92 TRIM31  
Affinity Capture-MS Homo sapiens
93 CIT 11113
Affinity Capture-MS Homo sapiens
94 VAMP5 10791
Affinity Capture-MS Homo sapiens
95 ATP6V0A2 23545
Co-fractionation Homo sapiens
96 EVA1B 55194
Affinity Capture-MS Homo sapiens
97 CAV1 857
Proximity Label-MS Homo sapiens
98 DERL1 79139
Proximity Label-MS Homo sapiens
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