Gene description for AKAP1
Gene name A kinase (PRKA) anchor protein 1
Gene symbol AKAP1
Other names/aliases AKAP
AKAP121
AKAP149
AKAP84
D-AKAP1
PPP1R43
PRKA1
SAKAP84
TDRD17
Species Homo sapiens
 Database cross references - AKAP1
ExoCarta ExoCarta_8165
Vesiclepedia VP_8165
Entrez Gene 8165
HGNC 367
MIM 602449
UniProt Q92667  
 AKAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for AKAP1
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A regulatory subunit binding GO:0034237 IPI
Biological Process
    apoptotic process GO:0006915 ISS
    antiviral innate immune response GO:0140374 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
 Experiment description of studies that identified AKAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AKAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PEX13  
Proximity Label-MS Homo sapiens
2 SLC25A13 10165
Proximity Label-MS Homo sapiens
3 ARHGAP1 392
Proximity Label-MS Homo sapiens
4 FAM73A  
Proximity Label-MS Homo sapiens
5 DNAJC11 55735
Proximity Label-MS Homo sapiens
6 STT3A 3703
Proximity Label-MS Homo sapiens
7 EIF3C 8663
Affinity Capture-MS Homo sapiens
8 PKP2 5318
Proximity Label-MS Homo sapiens
9 TMEM135  
Proximity Label-MS Homo sapiens
10 SMG7  
Proximity Label-MS Homo sapiens
11 TMEM263 90488
Proximity Label-MS Homo sapiens
12 MARC1  
Proximity Label-MS Homo sapiens
13 MSI1  
Proximity Label-MS Homo sapiens
14 CKB 1152
Proximity Label-MS Homo sapiens
15 C18orf32  
Proximity Label-MS Homo sapiens
16 QSOX2 169714
Proximity Label-MS Homo sapiens
17 IGF2BP3 10643
Proximity Label-MS Homo sapiens
18 ARL3 403
Proximity Label-MS Homo sapiens
19 ARHGEF2 9181
Proximity Label-MS Homo sapiens
20 IARS2 55699
Proximity Label-MS Homo sapiens
21 RAF1 5894
Proximity Label-MS Homo sapiens
22 APBB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 WSB2 55884
Proximity Label-MS Homo sapiens
24 ARMCX4  
Proximity Label-MS Homo sapiens
25 FUNDC2 65991
Proximity Label-MS Homo sapiens
26 TRABD 80305
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PSMD12 5718
Proximity Label-MS Homo sapiens
28 ABCD3 5825
Proximity Label-MS Homo sapiens
29 ETFA 2108
Proximity Label-MS Homo sapiens
30 MARCH5  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
31 VPS13D 55187
Proximity Label-MS Homo sapiens
32 NUS1  
Proximity Label-MS Homo sapiens
33 POLRMT 5442
Proximity Label-MS Homo sapiens
34 PTRH2 51651
Proximity Label-MS Homo sapiens
35 USP33  
Proximity Label-MS Homo sapiens
36 SLC25A46 91137
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
37 SLC30A9 10463
Proximity Label-MS Homo sapiens
38 FAM177A1 283635
Proximity Label-MS Homo sapiens
39 MLEC 9761
Proximity Label-MS Homo sapiens
40 FAM120A 23196
Proximity Label-MS Homo sapiens
41 SLC25A3 5250
Proximity Label-MS Homo sapiens
42 EXOC4 60412
Proximity Label-MS Homo sapiens
43 EMC1 23065
Proximity Label-MS Homo sapiens
44 UNC45A 55898
Proximity Label-MS Homo sapiens
45 BAG5 9529
Proximity Label-MS Homo sapiens
46 FARSA 2193
Proximity Label-MS Homo sapiens
47 MTCH2 23788
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
48 LRRC57 255252
Proximity Label-MS Homo sapiens
49 CAPZA1 829
Proximity Label-MS Homo sapiens
50 RAB14 51552
Proximity Label-MS Homo sapiens
51 MTFR1L 56181
Proximity Label-MS Homo sapiens
52 CSTF2 1478
Proximity Label-MS Homo sapiens
53 MICU2 221154
Proximity Label-MS Homo sapiens
54 COMTD1 118881
Proximity Label-MS Homo sapiens
55 KDELC2 143888
Proximity Label-MS Homo sapiens
56 ZFP41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 NUDC 10726
Proximity Label-MS Homo sapiens
58 CKAP5 9793
Affinity Capture-MS Homo sapiens
59 RAB24 53917
Proximity Label-MS Homo sapiens
60 SAR1B 51128
Proximity Label-MS Homo sapiens
61 UBXN4 23190
Proximity Label-MS Homo sapiens
62 PEX14 5195
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
63 XPOT 11260
Proximity Label-MS Homo sapiens
64 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
65 TNNI3  
Affinity Capture-MS Homo sapiens
66 HECTD1 25831
Affinity Capture-MS Homo sapiens
67 PRKAR1B  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
68 MICU1 10367
Proximity Label-MS Homo sapiens
69 CACYBP 27101
Proximity Label-MS Homo sapiens
70 REEP5 7905
Affinity Capture-MS Homo sapiens
71 NUCB2 4925
Proximity Label-MS Homo sapiens
72 CISD1 55847
Proximity Label-MS Homo sapiens
73 HSPA5 3309
Proximity Label-MS Homo sapiens
74 FAM83B  
Proximity Label-MS Homo sapiens
75 TARBP1 6894
Proximity Label-MS Homo sapiens
76 CDCA3 83461
Proximity Label-MS Homo sapiens
77 SUGT1 10910
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
78 TUBAL3 79861
Proximity Label-MS Homo sapiens
79 BCAP31 10134
Proximity Label-MS Homo sapiens
80 RAB29 8934
Proximity Label-MS Homo sapiens
81 CUL3 8452
Affinity Capture-MS Homo sapiens
82 MLX  
Proximity Label-MS Homo sapiens
83 FKBP8 23770
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
84 MYO19  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 AAAS 8086
Proximity Label-MS Homo sapiens
86 WIBG 84305
Affinity Capture-MS Homo sapiens
87 VPS13C 54832
Proximity Label-MS Homo sapiens
88 MYO1C 4641
Proximity Label-MS Homo sapiens
89 MAATS1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
90 RPA3 6119
Proximity Label-MS Homo sapiens
91 NME1 4830
Proximity Label-MS Homo sapiens
92 FAR2  
Proximity Label-MS Homo sapiens
93 HSPA1A 3303
Proximity Label-MS Homo sapiens
94 NCLN 56926
Proximity Label-MS Homo sapiens
95 HSPA4 3308
Proximity Label-MS Homo sapiens
96 GANAB 23193
Proximity Label-MS Homo sapiens
97 PEX3 8504
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
98 Prkar2a 19087
Affinity Capture-MS Mus musculus
99 CEPT1 10390
Proximity Label-MS Homo sapiens
100 Prkacb 18749
Affinity Capture-MS Mus musculus
101 STUB1 10273
Proximity Label-MS Homo sapiens
102 HK2 3099
Proximity Label-MS Homo sapiens
103 MIEF1  
Proximity Label-MS Homo sapiens
104 CPEB4  
Proximity Label-MS Homo sapiens
105 TRAK2 66008
Proximity Label-MS Homo sapiens
106 PEX11B 8799
Proximity Label-MS Homo sapiens
107 RAB27B 5874
Proximity Label-MS Homo sapiens
108 NAA20  
Affinity Capture-MS Homo sapiens
109 CDC37 11140
Proximity Label-MS Homo sapiens
110 SDF4 51150
Proximity Label-MS Homo sapiens
111 YARS 8565
Proximity Label-MS Homo sapiens
112 DHRS1 115817
Proximity Label-MS Homo sapiens
113 ITPR3 3710
Proximity Label-MS Homo sapiens
114 NUDT19 390916
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
115 ATAD3A 55210
Proximity Label-MS Homo sapiens
116 DRG1 4733
Proximity Label-MS Homo sapiens
117 ARMCX2 9823
Proximity Label-MS Homo sapiens
118 HM13 81502
Proximity Label-MS Homo sapiens
119 MIEF2  
Proximity Label-MS Homo sapiens
120 NDC1 55706
Proximity Label-MS Homo sapiens
121 SLC25A51  
Proximity Label-MS Homo sapiens
122 RAB11A 8766
Proximity Label-MS Homo sapiens
123 HSDL1 83693
Proximity Label-MS Homo sapiens
124 CISD2 493856
Proximity Label-MS Homo sapiens
125 RAB5C 5878
Proximity Label-MS Homo sapiens
126 PSMC4 5704
Proximity Label-MS Homo sapiens
127 PLD6  
Proximity Label-MS Homo sapiens
128 ARMC10 83787
Proximity Label-MS Homo sapiens
129 ASB14  
Affinity Capture-MS Homo sapiens
130 DERL1 79139
Proximity Label-MS Homo sapiens
131 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
132 KIF16B 55614
Proximity Label-MS Homo sapiens
133 LDHA 3939
Proximity Label-MS Homo sapiens
134 AFG3L2 10939
Proximity Label-MS Homo sapiens
135 PEX6  
Proximity Label-MS Homo sapiens
136 YKT6 10652
Proximity Label-MS Homo sapiens
137 SIAH2 6478
Affinity Capture-Western Homo sapiens
138 EIF2S3 1968
Proximity Label-MS Homo sapiens
139 NUP188 23511
Proximity Label-MS Homo sapiens
140 RBMS2 5939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 NUDCD3 23386
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
142 PRKCH 5583
Proximity Label-MS Homo sapiens
143 SURF4 6836
Proximity Label-MS Homo sapiens
144 PUM2 23369
Proximity Label-MS Homo sapiens
145 NENF 29937
Proximity Label-MS Homo sapiens
146 MARC2 54996
Proximity Label-MS Homo sapiens
147 YTHDF2 51441
Proximity Label-MS Homo sapiens
148 URI1  
Proximity Label-MS Homo sapiens
149 ABCD1 215
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 RAB3B 5865
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
151 POTEE 445582
Proximity Label-MS Homo sapiens
152 HP 3240
Affinity Capture-MS Homo sapiens
153 FIS1 51024
Proximity Label-MS Homo sapiens
154 PRR11  
Proximity Label-MS Homo sapiens
155 WDR41  
Proximity Label-MS Homo sapiens
156 HELZ 9931
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 STIP1 10963
Proximity Label-MS Homo sapiens
158 TBC1D15 64786
Proximity Label-MS Homo sapiens
159 DNAJC7 7266
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
160 PHB 5245
Affinity Capture-MS Homo sapiens
161 DDX6 1656
Affinity Capture-MS Homo sapiens
162 RAB6B 51560
Proximity Label-MS Homo sapiens
163 NPAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 USP7 7874
Affinity Capture-MS Homo sapiens
165 HK1 3098
Proximity Label-MS Homo sapiens
166 ARMCX3 51566
Proximity Label-MS Homo sapiens
167 TDRKH  
Proximity Label-MS Homo sapiens
168 LARP4B 23185
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
169 ATXN2 6311
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 CCT2 10576
Proximity Label-MS Homo sapiens
171 UGGT1 56886
Proximity Label-MS Homo sapiens
172 TTC37 9652
Proximity Label-MS Homo sapiens
173 CHMP7 91782
Proximity Label-MS Homo sapiens
174 PEX12  
Proximity Label-MS Homo sapiens
175 CORO7-PAM16 100529144
Proximity Label-MS Homo sapiens
176 TAB3 257397
Proximity Label-MS Homo sapiens
177 ATPAF1  
Proximity Label-MS Homo sapiens
178 BAG6 7917
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
179 SNAP47 116841
Proximity Label-MS Homo sapiens
180 RAP1A 5906
Proximity Label-MS Homo sapiens
181 PLK1 5347
Proximity Label-MS Homo sapiens
182 GK  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
183 PATL1 219988
Proximity Label-MS Homo sapiens
184 NME3 4832
Proximity Label-MS Homo sapiens
185 SLC25A17 10478
Proximity Label-MS Homo sapiens
186 MAOA 4128
Proximity Label-MS Homo sapiens
187 ACTR10 55860
Cross-Linking-MS (XL-MS) Homo sapiens
188 ABCB7 22
Proximity Label-MS Homo sapiens
189 FBXL4 26235
Proximity Label-MS Homo sapiens
190 RPN2 6185
Proximity Label-MS Homo sapiens
191 AIFM1 9131
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
192 CANX 821
Proximity Label-MS Homo sapiens
193 RAB5A 5868
Proximity Label-MS Homo sapiens
194 R3HDM1  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
195 MTX2 10651
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
196 PRKAR2A 5576
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
197 TNPO3 23534
Proximity Label-MS Homo sapiens
198 MTFR1  
Proximity Label-MS Homo sapiens
199 MTFR2  
Proximity Label-MS Homo sapiens
200 SCYL2 55681
Proximity Label-MS Homo sapiens
201 TOMM70A 9868
Proximity Label-MS Homo sapiens
202 BIRC6 57448
Proximity Label-MS Homo sapiens
203 R3HDM2  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
204 CABLES1  
Proximity Label-MS Homo sapiens
205 DSTN 11034
Proximity Label-MS Homo sapiens
206 RCN2 5955
Proximity Label-MS Homo sapiens
207 BNIP3 664
Proximity Label-MS Homo sapiens
208 LMNA 4000
Proximity Label-MS Homo sapiens
209 STX17 55014
Proximity Label-MS Homo sapiens
210 LARP4 113251
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
211 TOMM20 9804
Proximity Label-MS Homo sapiens
212 HUWE1 10075
Proximity Label-MS Homo sapiens
213 RAB18 22931
Proximity Label-MS Homo sapiens
214 SUN1 23353
Proximity Label-MS Homo sapiens
215 C17orf80 55028
Proximity Label-MS Homo sapiens
216 PRKCSH 5589
Proximity Label-MS Homo sapiens
217 PEX16 9409
Proximity Label-MS Homo sapiens
218 TTC1 7265
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
219 PRKY  
Affinity Capture-MS Homo sapiens
220 NME1-NME2 654364
Proximity Label-MS Homo sapiens
221 PEX19 5824
Proximity Label-MS Homo sapiens
222 PDCL 5082
Proximity Label-MS Homo sapiens
223 CALU 813
Proximity Label-MS Homo sapiens
224 TACC1 6867
Proximity Label-MS Homo sapiens
225 ARF5 381
Proximity Label-MS Homo sapiens
226 SMIM12  
Proximity Label-MS Homo sapiens
227 FKBP4 2288
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
228 PALD1 27143
Proximity Label-MS Homo sapiens
229 PRKCQ 5588
Proximity Label-MS Homo sapiens
230 PRKAR2B 5577