Gene description for ATAD1
Gene name ATPase family, AAA domain containing 1
Gene symbol ATAD1
Other names/aliases AFDC1
FNP001
THORASE
Species Homo sapiens
 Database cross references - ATAD1
ExoCarta ExoCarta_84896
Vesiclepedia VP_84896
Entrez Gene 84896
HGNC 25903
MIM 614452
UniProt Q8NBU5  
 ATAD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ATAD1
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 ISS
    membrane protein dislocase activity GO:0140567 IEA
Biological Process
    positive regulation of receptor internalization GO:0002092 ISS
    learning GO:0007612 ISS
    memory GO:0007613 ISS
    negative regulation of synaptic transmission, glutamatergic GO:0051967 ISS
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    extraction of mislocalized protein from mitochondrial outer membrane GO:0140570 IBA
    extraction of mislocalized protein from mitochondrial outer membrane GO:0140570 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    postsynaptic membrane GO:0045211 ISS
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ATAD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ATAD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
2 RELA 5970
Affinity Capture-MS Homo sapiens
3 PRMT8  
Affinity Capture-MS Homo sapiens
4 Rab5c 19345
Affinity Capture-MS Mus musculus
5 TPT1 7178
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 ZBTB2 57621
Affinity Capture-MS Homo sapiens
8 KLK15  
Affinity Capture-MS Homo sapiens
9 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GPR45  
Affinity Capture-MS Homo sapiens
11 FOXA1  
Affinity Capture-MS Homo sapiens
12 RPN2 6185
Proximity Label-MS Homo sapiens
13 AIFM1 9131
Proximity Label-MS Homo sapiens
14 MAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 VAPA 9218
Affinity Capture-MS Homo sapiens
16 SYAP1 94056
Affinity Capture-MS Homo sapiens
17 MARCH3 115123
Affinity Capture-MS Homo sapiens
18 RNF170 81790
Affinity Capture-MS Homo sapiens
19 SNX27 81609
Affinity Capture-MS Homo sapiens
20 TPM2 7169
Affinity Capture-MS Homo sapiens
21 Bmpr1a  
Affinity Capture-MS Mus musculus
22 FAM57A  
Affinity Capture-MS Homo sapiens
23 Rmdn3  
Affinity Capture-MS Mus musculus
24 BSCL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EGFR 1956
Affinity Capture-MS Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 GOSR1 9527
Affinity Capture-Western Homo sapiens
28 Tmed2 56334
Affinity Capture-MS Mus musculus
29 NDUFA4 4697
Affinity Capture-MS Homo sapiens
30 AQP3  
Affinity Capture-MS Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 FFAR1  
Affinity Capture-MS Homo sapiens
33 OCIAD1 54940
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
34 BABAM1 29086
Affinity Capture-MS Homo sapiens
35 NFKB1 4790
Affinity Capture-MS Homo sapiens
36 ANKS6 203286
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 FIS1 51024
Proximity Label-MS Homo sapiens
39 SREBF2 6721
Positive Genetic Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 LMNB1 4001
Affinity Capture-MS Homo sapiens
42 COQ9  
Affinity Capture-MS Homo sapiens
43 RAB9A 9367
Proximity Label-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 CIT 11113
Affinity Capture-MS Homo sapiens
46 USP19 10869
Affinity Capture-MS Homo sapiens
47 PEX14 5195
Co-purification Homo sapiens
48 METAP2 10988
Affinity Capture-MS Homo sapiens
49 PRC1 9055
Affinity Capture-MS Homo sapiens
50 RPN1 6184
Proximity Label-MS Homo sapiens
51 VDAC1 7416
Affinity Capture-MS Homo sapiens
52 CGRRF1  
Affinity Capture-MS Homo sapiens
53 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RNF185  
Affinity Capture-MS Homo sapiens
55 PLD6  
Affinity Capture-MS Homo sapiens
56 AKAP1 8165
Proximity Label-MS Homo sapiens
57 SEC61B 10952
Proximity Label-MS Homo sapiens
58 REV3L  
Synthetic Growth Defect Homo sapiens
View the network image/svg+xml
 Pathways in which ATAD1 is involved
PathwayEvidenceSource
Class I peroxisomal membrane protein import TAS Reactome
Protein localization TAS Reactome





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