Gene description for Rab5c
Gene name RAB5C, member RAS oncogene family
Gene symbol Rab5c
Other names/aliases AI326010
Rabl
Species Mus musculus
 Database cross references - Rab5c
ExoCarta ExoCarta_19345
Entrez Gene 19345
UniProt P35278  
 Rab5c identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Rab5c
Molecular Function
    GDP binding GO:0019003 ISO
    protein binding GO:0005515 IPI
    GTPase activity GO:0003924 TAS
    GTP binding GO:0005525 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    Rab protein signal transduction GO:0032482 IBA
    plasma membrane to endosome transport GO:0048227 ISO
    regulation of endocytosis GO:0030100 IDA
    metabolic process GO:0008152 ISO
    protein transport GO:0015031 IEA
    small GTPase mediated signal transduction GO:0007264 IEA
    endosome organization GO:0007032 IDA
    transport GO:0006810 IEA
Subcellular Localization
    lipid particle GO:0005811 ISO
    endosome GO:0005768 IEA
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 IBA
    early endosome GO:0005769 IBA
    extracellular exosome GO:0070062 ISO
    lysosomal membrane GO:0005765 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    cytoplasmic vesicle GO:0031410 ISO
    endocytic vesicle GO:0030139 IDA
 Experiment description of studies that identified Rab5c in exosomes
1
Experiment ID 89
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 39
ISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Rab5c
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rab5c is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis IEA Reactome





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