Gene description for Rab5c
Gene name RAB5C, member RAS oncogene family
Gene symbol Rab5c
Other names/aliases AI326010
Rabl
Species Mus musculus
 Database cross references - Rab5c
ExoCarta ExoCarta_19345
Vesiclepedia VP_19345
Entrez Gene 19345
UniProt P35278  
 Rab5c identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab5c
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytosis GO:0006897 IBA
    endosome organization GO:0007032 IDA
    protein transport GO:0015031 IEA
    regulation of endocytosis GO:0030100 IDA
    plasma membrane to endosome transport GO:0048227 ISO
Subcellular Localization
    endosome GO:0005768 ISO
    early endosome GO:0005769 IBA
    lipid droplet GO:0005811 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    endomembrane system GO:0012505 IBA
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 IDA
    synaptic vesicle membrane GO:0030672 ISO
    synaptic vesicle membrane GO:0030672 ISO
    early endosome membrane GO:0031901 IEA
    melanosome GO:0042470 IEA
 Experiment description of studies that identified Rab5c in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab5c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 CHMP2A 27243
Affinity Capture-MS Homo sapiens
3 ABCG2 9429
Affinity Capture-MS Homo sapiens
4 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
5 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
6 Lmna 16905
Affinity Capture-MS Mus musculus
7 NDUFS1 4719
Affinity Capture-MS Homo sapiens
8 TMUB1  
Affinity Capture-MS Homo sapiens
9 SPNS1 83985
Affinity Capture-MS Homo sapiens
10 MRPL27 51264
Affinity Capture-MS Homo sapiens
11 METTL5  
Affinity Capture-MS Homo sapiens
12 ABCD1 215
Affinity Capture-MS Homo sapiens
13 Tmem173  
Proximity Label-MS Mus musculus
14 CLCN7 1186
Affinity Capture-MS Homo sapiens
15 RNF5  
Affinity Capture-MS Homo sapiens
16 TMEM199  
Affinity Capture-MS Homo sapiens
17 NDUFA7 4701
Affinity Capture-MS Homo sapiens
18 RPS10 6204
Affinity Capture-MS Homo sapiens
19 PNPLA6 10908
Affinity Capture-MS Homo sapiens
20 TMEM55B 90809
Affinity Capture-MS Homo sapiens
21 CHMP2B 25978
Affinity Capture-MS Homo sapiens
22 Mapt  
Affinity Capture-MS Mus musculus
23 RAB5B 5869
Affinity Capture-MS Homo sapiens
24 EPS15L1 58513
Affinity Capture-MS Homo sapiens
25 SUN2 25777
Affinity Capture-MS Homo sapiens
26 GALNT2 2590
Affinity Capture-MS Homo sapiens
27 NDUFV1 4723
Affinity Capture-MS Homo sapiens
28 PHB 5245
Affinity Capture-MS Homo sapiens
29 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
30 HCN2  
Affinity Capture-MS Homo sapiens
31 ARHGAP39  
Affinity Capture-MS Homo sapiens
32 TFRC 7037
Affinity Capture-MS Homo sapiens
33 NDUFS5 4725
Affinity Capture-MS Homo sapiens
34 CYBRD1 79901
Affinity Capture-MS Homo sapiens
35 NDUFB1 4707
Affinity Capture-MS Homo sapiens
36 STOML2 30968
Affinity Capture-MS Homo sapiens
37 GOLIM4 27333
Affinity Capture-MS Homo sapiens
38 TAP1 6890
Affinity Capture-MS Homo sapiens
39 ATAD1 84896
Affinity Capture-MS Homo sapiens
40 GMDS 2762
Affinity Capture-MS Homo sapiens
41 TMEM43 79188
Affinity Capture-MS Homo sapiens
42 TBL2 26608
Affinity Capture-MS Homo sapiens
43 STOM 2040
Affinity Capture-MS Homo sapiens
44 TMEM165 55858
Affinity Capture-MS Homo sapiens
45 CHCHD3 54927
Affinity Capture-MS Homo sapiens
46 ACIN1 22985
Affinity Capture-MS Homo sapiens
47 PTCD3 55037
Affinity Capture-MS Homo sapiens
48 UBAC2 337867
Affinity Capture-MS Homo sapiens
49 SLC9A1 6548
Affinity Capture-MS Homo sapiens
50 SPRR1B 6699
Affinity Capture-MS Homo sapiens
51 UQCRC1 7384
Affinity Capture-MS Homo sapiens
52 STX5 6811
Affinity Capture-MS Homo sapiens
53 ERMP1 79956
Affinity Capture-MS Homo sapiens
54 FLOT2 2319
Affinity Capture-MS Homo sapiens
55 VDAC2 7417
Affinity Capture-MS Homo sapiens
56 RMDN3 55177
Affinity Capture-MS Homo sapiens
57 GPRC5A 9052
Affinity Capture-MS Homo sapiens
58 RAB37 326624
Affinity Capture-MS Homo sapiens
59 BRF1  
Affinity Capture-MS Homo sapiens
60 COX5B 1329
Affinity Capture-MS Homo sapiens
61 Eed  
Affinity Capture-MS Mus musculus
62 VAPB 9217
Affinity Capture-MS Homo sapiens
63 PIP 5304
Affinity Capture-MS Homo sapiens
64 SLC35E1 79939
Affinity Capture-MS Homo sapiens
65 PDIA3 2923
Affinity Capture-MS Homo sapiens
66 FKBP8 23770
Affinity Capture-MS Homo sapiens
67 HOOK3 84376
Affinity Capture-MS Homo sapiens
68 COX6B1 1340
Affinity Capture-MS Homo sapiens
69 NDUFA10 4705
Affinity Capture-MS Homo sapiens
70 OCIAD1 54940
Affinity Capture-MS Homo sapiens
71 GDI2 2665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 COG1 9382
Affinity Capture-MS Homo sapiens
73 CCDC127  
Affinity Capture-MS Homo sapiens
74 PCDH7 5099
Affinity Capture-MS Homo sapiens
75 SOAT1 6646
Affinity Capture-MS Homo sapiens
76 MYOF 26509
Affinity Capture-MS Homo sapiens
77 ATP6V1A 523
Affinity Capture-MS Homo sapiens
78 INO80  
Affinity Capture-MS Homo sapiens
79 KTN1 3895
Affinity Capture-MS Homo sapiens
80 VDAC1 7416
Affinity Capture-MS Homo sapiens
81 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
82 STX10 8677
Affinity Capture-MS Homo sapiens
83 KIDINS220 57498
Affinity Capture-MS Homo sapiens
84 NDUFV2 4729
Affinity Capture-MS Homo sapiens
85 CHM 1121
Affinity Capture-MS Homo sapiens
86 Tia1  
Affinity Capture-MS Mus musculus
87 TPM4 7171
Affinity Capture-MS Homo sapiens
88 FLOT1 10211
Affinity Capture-MS Homo sapiens
89 Fancd2  
Affinity Capture-MS Mus musculus
90 IMMT 10989
Affinity Capture-MS Homo sapiens
91 PHB2 11331
Affinity Capture-MS Homo sapiens
92 SLC38A2 54407
Affinity Capture-MS Homo sapiens
93 TMEM57  
Affinity Capture-MS Homo sapiens
94 JUP 3728
Affinity Capture-MS Homo sapiens
95 PIEZO1 9780
Affinity Capture-MS Homo sapiens
96 ERLIN1 10613
Affinity Capture-MS Homo sapiens
97 GOSR1 9527
Affinity Capture-MS Homo sapiens
98 XPR1 9213
Affinity Capture-MS Homo sapiens
99 MRPS31  
Affinity Capture-MS Homo sapiens
100 Atxn1  
Affinity Capture-MS Mus musculus
101 Kctd13  
Affinity Capture-MS Mus musculus
102 DNAAF5 54919
Affinity Capture-MS Homo sapiens
103 Rab7 19349
Co-fractionation Mus musculus
104 ERLIN2 11160
Affinity Capture-MS Homo sapiens
105 NDUFA12 55967
Affinity Capture-MS Homo sapiens
106 SLC35F6 54978
Affinity Capture-MS Homo sapiens
107 MMGT1 93380
Affinity Capture-MS Homo sapiens
108 Rpa1 68275
Co-fractionation Mus musculus
109 NDUFA2 4695
Affinity Capture-MS Homo sapiens
110 TMEM109 79073
Affinity Capture-MS Homo sapiens
111 RAB5C 5878
Affinity Capture-MS Homo sapiens
112 NDUFB11 54539
Affinity Capture-MS Homo sapiens
113 MRPL55  
Affinity Capture-MS Homo sapiens
114 NCBP2-AS2  
Affinity Capture-MS Homo sapiens
115 CKAP4 10970
Affinity Capture-MS Homo sapiens
116 PTRF 284119
Affinity Capture-MS Homo sapiens
117 CAV1 857
Affinity Capture-MS Homo sapiens
118 NDUFS6  
Affinity Capture-MS Homo sapiens
119 HLA-C 3107
Affinity Capture-MS Homo sapiens
120 NDUFB4 4710
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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