Gene description for Rpa1
Gene name replication protein A1
Gene symbol Rpa1
Other names/aliases 5031405K23Rik
70kDa
AA589576
AW557552
RF-A
RP-A
Rpa
Species Mus musculus
 Database cross references - Rpa1
ExoCarta ExoCarta_68275
Vesiclepedia VP_68275
Entrez Gene 68275
UniProt Q8VEE4  
 Rpa1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpa1
Molecular Function
    chromatin binding GO:0003682 IDA
    metal ion binding GO:0046872 IEA
    DNA binding GO:0003677 IEA
    damaged DNA binding GO:0003684 ISO
    single-stranded DNA binding GO:0003697 ISO
    nucleic acid binding GO:0003676 IEA
Biological Process
    mismatch repair GO:0006298 ISO
    double-strand break repair via homologous recombination GO:0000724 ISO
    in utero embryonic development GO:0001701 IMP
    homeostasis of number of cells within a tissue GO:0048873 IMP
    telomere maintenance GO:0000723 ISO
    DNA replication GO:0006260 ISO
    nucleotide-excision repair GO:0006289 ISO
    cellular response to DNA damage stimulus GO:0006974 IEA
    base-excision repair GO:0006284 ISO
    hemopoiesis GO:0030097 IMP
    positive regulation of cell proliferation GO:0008284 IMP
    cell cycle checkpoint GO:0000075 ISO
    chromosome organization GO:0051276 IMP
    DNA repair GO:0006281 ISO
    meiotic nuclear division GO:0007126 IDA
    DNA recombination GO:0006310 IEA
Subcellular Localization
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 ISO
    condensed chromosome GO:0000793 IDA
    DNA replication factor A complex GO:0005662 ISO
    lateral element GO:0000800 IDA
    condensed nuclear chromosome GO:0000794 IDA
    PML body GO:0016605 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Rpa1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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