Gene description for TMEM55B
Gene name transmembrane protein 55B
Gene symbol TMEM55B
Other names/aliases C14orf9
Species Homo sapiens
 Database cross references - TMEM55B
ExoCarta ExoCarta_90809
Vesiclepedia VP_90809
Entrez Gene 90809
HGNC 19299
MIM 609865
UniProt Q86T03  
 TMEM55B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TMEM55B
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 IBA
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 IDA
    phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity GO:0034597 TAS
Biological Process
    phospholipid metabolic process GO:0006644 TAS
    response to sterol depletion GO:0006991 IDA
    cholesterol metabolic process GO:0008203 IMP
    lysosome localization GO:0032418 IMP
    phosphatidylinositol dephosphorylation GO:0046856 IBA
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    proton-transporting V-type ATPase complex assembly GO:0070070 ISS
    regulation of signal transduction by p53 class mediator GO:1901796 TAS
    positive regulation of TORC1 signaling GO:1904263 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    phagocytic vesicle membrane GO:0030670 IBA
    phagocytic vesicle membrane GO:0030670 ISS
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IDA
    late endosome membrane GO:0031902 TAS
 Experiment description of studies that identified TMEM55B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMEM55B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F10 2159
Affinity Capture-MS Homo sapiens
2 Rab5c 19345
Affinity Capture-MS Mus musculus
3 SPAG9 9043
Affinity Capture-MS Homo sapiens
4 TPD52L2 7165
Affinity Capture-MS Homo sapiens
5 ZNFX1 57169
Affinity Capture-MS Homo sapiens
6 FAF2 23197
Affinity Capture-MS Homo sapiens
7 SLC10A6  
Two-hybrid Homo sapiens
8 NEDD4 4734
Affinity Capture-MS Homo sapiens
9 RIC3  
Affinity Capture-MS Homo sapiens
10 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 Vps4b 20479
Affinity Capture-MS Mus musculus
13 OPALIN  
Affinity Capture-MS Homo sapiens
14 WWP1 11059
Affinity Capture-MS Homo sapiens
15 SCAMP1 9522
Affinity Capture-MS Homo sapiens
16 EMC7 56851
Affinity Capture-MS Homo sapiens
17 LAMP1 3916
Affinity Capture-MS Homo sapiens
18 GLMN 11146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GOLT1B 51026
Affinity Capture-MS Homo sapiens
20 TMEM63A 9725
Affinity Capture-MS Homo sapiens
21 LAMTOR2 28956
Affinity Capture-MS Homo sapiens
22 C10orf32  
Affinity Capture-MS Homo sapiens
23 LAMP2 3920
Proximity Label-MS Homo sapiens
24 SAMD1  
Affinity Capture-MS Homo sapiens
25 VPS13C 54832
Affinity Capture-MS Homo sapiens
26 DFFB  
Affinity Capture-MS Homo sapiens
27 RAB39B 116442
Affinity Capture-MS Homo sapiens
28 RILPL1 353116
Two-hybrid Homo sapiens
29 TMEM55B 90809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ARL8B 55207
Affinity Capture-MS Homo sapiens
31 EIF2B5 8893
Affinity Capture-MS Homo sapiens
32 TTC30B  
Affinity Capture-MS Homo sapiens
33 MMGT1 93380
Affinity Capture-MS Homo sapiens
34 LAMTOR3 8649
Affinity Capture-MS Homo sapiens
35 TPD52 7163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 RNF213 57674
Affinity Capture-MS Homo sapiens
37 PLEKHB2 55041
Two-hybrid Homo sapiens
38 BIRC6 57448
Affinity Capture-MS Homo sapiens
39 RAB2A 5862
Affinity Capture-MS Homo sapiens
40 SPPL2B 56928
Affinity Capture-MS Homo sapiens
41 SCGN  
Affinity Capture-MS Homo sapiens
42 RAB9A 9367
Proximity Label-MS Homo sapiens
43 EIF2B2 8892
Affinity Capture-MS Homo sapiens
44 OC90  
Affinity Capture-MS Homo sapiens
45 ASPH 444
Affinity Capture-MS Homo sapiens
46 TMEM106B 54664
Affinity Capture-MS Homo sapiens
47 EIF2B3 8891
Affinity Capture-MS Homo sapiens
48 NDUFB11 54539
Affinity Capture-MS Homo sapiens
49 SLC6A15 55117
Affinity Capture-MS Homo sapiens
50 NFATC3 4775
Affinity Capture-MS Homo sapiens
51 SLC1A1 6505
Affinity Capture-MS Homo sapiens
52 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
53 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TMEM171  
Affinity Capture-MS Homo sapiens
55 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 RNF13 11342
Affinity Capture-MS Homo sapiens
57 LOH12CR1 118426
Affinity Capture-MS Homo sapiens
58 PIN4 5303
Affinity Capture-MS Homo sapiens
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