Gene description for RNF213
Gene name ring finger protein 213
Gene symbol RNF213
Other names/aliases ALO17
C17orf27
KIAA1618
MYMY2
MYSTR
NET57
Species Homo sapiens
 Database cross references - RNF213
ExoCarta ExoCarta_57674
Vesiclepedia VP_57674
Entrez Gene 57674
HGNC 14539
MIM 613768
UniProt Q63HN8  
 RNF213 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for RNF213
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IDA
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    angiogenesis GO:0001525 IMP
    sprouting angiogenesis GO:0002040 IBA
    sprouting angiogenesis GO:0002040 IMP
    immune system process GO:0002376 IEA
    chromatin remodeling GO:0006338 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    protein ubiquitination GO:0016567 IDA
    regulation of lipid metabolic process GO:0019216 IDA
    defense response to bacterium GO:0042742 IDA
    protein autoubiquitination GO:0051865 IDA
    protein K63-linked ubiquitination GO:0070534 IDA
    xenophagy GO:0098792 IDA
    lipid ubiquitination GO:0120323 IDA
    lipid droplet formation GO:0140042 IDA
    chromatin looping GO:0140588 IEA
    negative regulation of non-canonical Wnt signaling pathway GO:2000051 IBA
    negative regulation of non-canonical Wnt signaling pathway GO:2000051 IMP
Subcellular Localization
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified RNF213 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RNF213
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 RNASEH2B  
Affinity Capture-MS Homo sapiens
5 SLC35G1  
Affinity Capture-MS Homo sapiens
6 ATG5 9474
Proximity Label-MS Homo sapiens
7 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HCST  
Affinity Capture-MS Homo sapiens
9 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 REL 5966
Two-hybrid Homo sapiens
12 TRIM66  
Affinity Capture-MS Homo sapiens
13 IGHM 3507
Affinity Capture-MS Homo sapiens
14 FPR1  
Affinity Capture-MS Homo sapiens
15 TMEM9B 56674
Affinity Capture-MS Homo sapiens
16 UBC 7316
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 MRPL50 54534
Affinity Capture-MS Homo sapiens
18 PTPN1 5770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
19 UBE2N 7334
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 FFAR1  
Affinity Capture-MS Homo sapiens
21 KIAA1429 25962
Affinity Capture-MS Homo sapiens
22 FIS1 51024
Proximity Label-MS Homo sapiens
23 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
24 MCM2 4171
Affinity Capture-MS Homo sapiens
25 HLA-B 3106
Affinity Capture-MS Homo sapiens
26 FAM174A 345757
Affinity Capture-MS Homo sapiens
27 OSBP 5007
Co-fractionation Homo sapiens
28 FBXW7  
Affinity Capture-MS Homo sapiens
29 KRT40  
Two-hybrid Homo sapiens
30 GSPT1 2935
Co-fractionation Homo sapiens
31 TACSTD2 4070
Affinity Capture-MS Homo sapiens
32 GPR17 2840
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 GOLGA2 2801
Two-hybrid Homo sapiens
35 CHMP4C 92421
Affinity Capture-MS Homo sapiens
36 BSG 682
Affinity Capture-MS Homo sapiens
37 HS2ST1 9653
Affinity Capture-MS Homo sapiens
38 GCGR  
Affinity Capture-MS Homo sapiens
39 C19orf38  
Affinity Capture-MS Homo sapiens
40 CTDP1 9150
Affinity Capture-MS Homo sapiens
41 TMEM55B 90809
Affinity Capture-MS Homo sapiens
42 NONO 4841
Cross-Linking-MS (XL-MS) Homo sapiens
43 VSIG4  
Affinity Capture-MS Homo sapiens
44 RNF213 57674
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
45 Ube2k 53323
Affinity Capture-MS Mus musculus
46 NPDC1  
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 C5AR1  
Affinity Capture-MS Homo sapiens
49 PML 5371
Affinity Capture-MS Homo sapiens
50 UBE2D3 7323
Reconstituted Complex Homo sapiens
51 CYLD  
Affinity Capture-MS Homo sapiens
52 LOC388882  
Affinity Capture-MS Homo sapiens
53 UPK2 7379
Affinity Capture-MS Homo sapiens
54 CD40 958
Affinity Capture-MS Homo sapiens
55 GAN 8139
Affinity Capture-MS Homo sapiens
56 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 NPTN 27020
Affinity Capture-MS Homo sapiens
58 MGARP  
Affinity Capture-MS Homo sapiens
59 CLEC4A  
Affinity Capture-MS Homo sapiens
60 EGFR 1956
Affinity Capture-MS Homo sapiens
61 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 OCIAD1 54940
Proximity Label-MS Homo sapiens
63 AURKB 9212
Affinity Capture-MS Homo sapiens
64 UBE2D2 7322
Reconstituted Complex Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 RELT  
Affinity Capture-MS Homo sapiens
67 GPR45  
Affinity Capture-MS Homo sapiens
68 TRIM27  
Two-hybrid Homo sapiens
69 CD244 51744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 Rpl35 66489
Affinity Capture-MS Mus musculus
72 MGEA5 10724
Co-fractionation Homo sapiens
73 UBE2U  
Two-hybrid Homo sapiens
74 TRIP6 7205
Two-hybrid Homo sapiens
75 STK16 8576
Affinity Capture-MS Homo sapiens
76 OPALIN  
Affinity Capture-MS Homo sapiens
77 MFSD4  
Affinity Capture-MS Homo sapiens
78 OSTM1 28962
Affinity Capture-MS Homo sapiens
79 MEN1 4221
Affinity Capture-MS Homo sapiens
80 EFNB1 1947
Affinity Capture-MS Homo sapiens
81 UBXN11  
Two-hybrid Homo sapiens
82 BAG5 9529
Affinity Capture-MS Homo sapiens
83 Kif4  
Affinity Capture-MS Mus musculus
84 TRIM23 373
Two-hybrid Homo sapiens
85 CIT 11113
Affinity Capture-MS Homo sapiens
86 HSPA4L 22824
Co-fractionation Homo sapiens
87 POLR1D 51082
Affinity Capture-MS Homo sapiens
88 AR 367
Affinity Capture-MS Homo sapiens
89 ATOH1  
Affinity Capture-MS Homo sapiens
90 PLAC9 219348
Two-hybrid Homo sapiens
91 CUL4A 8451
Affinity Capture-MS Homo sapiens
92 HLA-C 3107
Affinity Capture-MS Homo sapiens
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