Gene description for OSTM1
Gene name osteopetrosis associated transmembrane protein 1
Gene symbol OSTM1
Other names/aliases GIPN
GL
HSPC019
OPTB5
Species Homo sapiens
 Database cross references - OSTM1
ExoCarta ExoCarta_28962
Vesiclepedia VP_28962
Entrez Gene 28962
HGNC 21652
MIM 607649
UniProt Q86WC4  
 OSTM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for OSTM1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    osteoclast differentiation GO:0030316 IBA
    transepithelial chloride transport GO:0030321 IDA
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    cytosol GO:0005829 IBA
    chloride channel complex GO:0034707 IPI
 Experiment description of studies that identified OSTM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for OSTM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA4A 7277
Affinity Capture-MS Homo sapiens
2 PEX6  
Affinity Capture-MS Homo sapiens
3 ATR  
Affinity Capture-MS Homo sapiens
4 LIG4 3981
Two-hybrid Homo sapiens
5 GUF1  
Affinity Capture-MS Homo sapiens
6 TUBB6 84617
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CLCN7 1186
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 DECR2 26063
Affinity Capture-MS Homo sapiens
10 TUBB 203068
Affinity Capture-MS Homo sapiens
11 METTL17  
Proximity Label-MS Homo sapiens
12 TRAF2 7186
Affinity Capture-MS Homo sapiens
13 SLC25A23  
Affinity Capture-MS Homo sapiens
14 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CXCR4 7852
Affinity Capture-MS Homo sapiens
16 FASTKD3  
Affinity Capture-MS Homo sapiens
17 TARBP1 6894
Affinity Capture-MS Homo sapiens
18 SCGB1D1  
Affinity Capture-MS Homo sapiens
19 BIRC2  
Affinity Capture-MS Homo sapiens
20 ACAD10  
Affinity Capture-MS Homo sapiens
21 HARS2 23438
Affinity Capture-MS Homo sapiens
22 RGS19 10287
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
23 RTCA 8634
Affinity Capture-MS Homo sapiens
24 ASAH1 427
Affinity Capture-MS Homo sapiens
25 ACTA2 59
Affinity Capture-MS Homo sapiens
26 RGS17  
Two-hybrid Homo sapiens
27 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 STK39 27347
Affinity Capture-MS Homo sapiens
29 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
30 TUBB3 10381
Affinity Capture-MS Homo sapiens
31 TUBB4B 10383
Affinity Capture-MS Homo sapiens
32 LAMP2 3920
Proximity Label-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 NSUN3  
Affinity Capture-MS Homo sapiens
35 PDXDC2P  
Affinity Capture-MS Homo sapiens
36 INTS1 26173
Affinity Capture-MS Homo sapiens
37 DDX19B 11269
Affinity Capture-MS Homo sapiens
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 TUBB4A 10382
Affinity Capture-MS Homo sapiens
40 TBCD 6904
Affinity Capture-MS Homo sapiens
41 NLRX1 79671
Affinity Capture-MS Homo sapiens
42 ILVBL 10994
Affinity Capture-MS Homo sapiens
43 PEX1 5189
Affinity Capture-MS Homo sapiens
44 HTT 3064
Affinity Capture-MS Homo sapiens
45 TAZ  
Affinity Capture-MS Homo sapiens
46 RNF213 57674
Affinity Capture-MS Homo sapiens
47 PPTC7 160760
Affinity Capture-MS Homo sapiens
48 TUBA1A 7846
Affinity Capture-MS Homo sapiens
49 TUBB2B 347733
Affinity Capture-MS Homo sapiens
50 SLC25A6 293
Affinity Capture-MS Homo sapiens
51 FAM118B  
Affinity Capture-MS Homo sapiens
52 SLC25A5 292
Affinity Capture-MS Homo sapiens
53 EXOSC2 23404
Affinity Capture-Western Homo sapiens
54 ABHD11 83451
Affinity Capture-MS Homo sapiens
55 JAK1 3716
Affinity Capture-MS Homo sapiens
56 AGPAT5 55326
Affinity Capture-MS Homo sapiens
57 FBXO2 26232
Affinity Capture-MS Homo sapiens
58 EXOSC3 51010
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 XIAP  
Affinity Capture-MS Homo sapiens
60 RGS20 8601
Two-hybrid Homo sapiens
61 EXOSC5 56915
Affinity Capture-Western Homo sapiens
62 SLC25A4 291
Affinity Capture-MS Homo sapiens
63 FLVCR1 28982
Affinity Capture-MS Homo sapiens
64 GPSM1 26086
Reconstituted Complex Homo sapiens
65 UFSP2 55325
Affinity Capture-MS Homo sapiens
66 TUBA1C 84790
Affinity Capture-MS Homo sapiens
67 ARFGEF1 10565
Affinity Capture-MS Homo sapiens
68 BIRC7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SFXN3 81855
Affinity Capture-MS Homo sapiens
71 ZWILCH 55055
Affinity Capture-MS Homo sapiens
72 SLC25A30 253512
Affinity Capture-MS Homo sapiens
73 EXOSC4 54512
Affinity Capture-Western Homo sapiens
74 TMTC4  
Affinity Capture-MS Homo sapiens
75 RTEL1 51750
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which OSTM1 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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