Gene description for EXOSC5
Gene name exosome component 5
Gene symbol EXOSC5
Other names/aliases RRP41B
RRP46
Rrp46p
hRrp46p
p12B
Species Homo sapiens
 Database cross references - EXOSC5
ExoCarta ExoCarta_56915
Vesiclepedia VP_56915
Entrez Gene 56915
HGNC 24662
MIM 606492
UniProt Q9NQT4  
 EXOSC5 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EXOSC5
Molecular Function
    exoribonuclease activity GO:0004532 IDA
    3'-5'-exoribonuclease activity GO:0000175 NAS
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    polyadenylation-dependent snoRNA 3'-end processing GO:0071051 IBA
    U4 snRNA 3'-end processing GO:0034475 IBA
    nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' GO:0034427 IBA
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    rRNA 3'-end processing GO:0031125 IBA
    exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0043928 TAS
    defense response to virus GO:0051607 IMP
    gene expression GO:0010467 TAS
    rRNA processing GO:0006364 NAS
    nuclear mRNA surveillance GO:0071028 IBA
    rRNA catabolic process GO:0016075 IBA
    DNA deamination GO:0045006 IDA
    RNA phosphodiester bond hydrolysis, exonucleolytic GO:0090503 NAS
Subcellular Localization
    exosome (RNase complex) GO:0000178 IDA
    cytoplasm GO:0005737 NAS
    cytoplasmic exosome (RNase complex) GO:0000177 IBA
    nucleolus GO:0005730 NAS
    transcriptionally active chromatin GO:0035327 IMP
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    nuclear exosome (RNase complex) GO:0000176 IBA
 Experiment description of studies that identified EXOSC5 in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOSC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LSM5 23658
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 KIAA1217 56243
Two-hybrid Homo sapiens
3 NUP210 23225
Two-hybrid Homo sapiens
4 SFPQ 6421
Two-hybrid Homo sapiens
5 CDK5RAP1  
Affinity Capture-MS Homo sapiens
6 EXOSC1 51013
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
7 EXOSC3 51010
Affinity Capture-MS Homo sapiens
8 PKM 5315
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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