Gene description for EXOSC5
Gene name exosome component 5
Gene symbol EXOSC5
Other names/aliases RRP41B
RRP46
Rrp46p
hRrp46p
p12B
Species Homo sapiens
 Database cross references - EXOSC5
ExoCarta ExoCarta_56915
Vesiclepedia VP_56915
Entrez Gene 56915
HGNC 24662
MIM 606492
UniProt Q9NQT4  
 EXOSC5 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EXOSC5
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 NAS
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 IBA
    RNA exonuclease activity GO:0004532 IDA
    protein binding GO:0005515 IPI
Biological Process
    rRNA processing GO:0006364 NAS
    RNA processing GO:0006396 IDA
    RNA catabolic process GO:0006401 IDA
    mRNA catabolic process GO:0006402 IMP
    rRNA catabolic process GO:0016075 IBA
    U4 snRNA 3'-end processing GO:0034475 IBA
    DNA deamination GO:0045006 IDA
    defense response to virus GO:0051607 IMP
    nuclear mRNA surveillance GO:0071028 IBA
    poly(A)-dependent snoRNA 3'-end processing GO:0071051 IBA
Subcellular Localization
    nuclear exosome (RNase complex) GO:0000176 IBA
    nuclear exosome (RNase complex) GO:0000176 NAS
    cytoplasmic exosome (RNase complex) GO:0000177 IBA
    cytoplasmic exosome (RNase complex) GO:0000177 NAS
    exosome (RNase complex) GO:0000178 IDA
    exosome (RNase complex) GO:0000178 NAS
    euchromatin GO:0000791 IMP
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 NAS
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nucleolar exosome (RNase complex) GO:0101019 NAS
 Experiment description of studies that identified EXOSC5 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOSC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
3 EXOSC7 23016
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 WDR3  
Affinity Capture-MS Homo sapiens
5 DIS3 22894
Affinity Capture-MS Homo sapiens
6 PKM 5315
Two-hybrid Homo sapiens
7 PSMA4 5685
Co-fractionation Homo sapiens
8 REL 5966
Two-hybrid Homo sapiens
9 TEX11  
Two-hybrid Homo sapiens
10 FCHO1  
Two-hybrid Homo sapiens
11 CDK5RAP1  
Two-hybrid Homo sapiens
12 TERF2IP 54386
Affinity Capture-MS Homo sapiens
13 EIF4E2  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 DECR1 1666
Affinity Capture-MS Homo sapiens
16 LSM5 23658
Two-hybrid Homo sapiens
17 USP36  
Affinity Capture-MS Homo sapiens
18 PSIP1 11168
Co-fractionation Homo sapiens
19 TOP2A 7153
Affinity Capture-MS Homo sapiens
20 EXOSC2 23404
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C1D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Htatsf1  
Two-hybrid Mus musculus
23 CPSF7 79869
Two-hybrid Homo sapiens
24 NUP210 23225
Two-hybrid Homo sapiens
25 NPM1 4869
Affinity Capture-MS Homo sapiens
26 CALCOCO2  
Two-hybrid Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 KRT31 3881
Two-hybrid Homo sapiens
29 EXOSC4 54512
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 Dynlt1b  
Two-hybrid Mus musculus
31 LIPG 9388
Two-hybrid Homo sapiens
32 EXOSC6 118460
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MPHOSPH6 10200
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 RPL19 6143
Affinity Capture-MS Homo sapiens
35 EXOSC9 5393
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DMRTB1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 KIAA1217 56243
Two-hybrid Homo sapiens
38 DIS3L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CEP55 55165
Two-hybrid Homo sapiens
40 FHL3 2275
Two-hybrid Homo sapiens
41 MEPCE 56257
Affinity Capture-MS Homo sapiens
42 PEG10 23089
Two-hybrid Homo sapiens
43 Nudt21 68219
Two-hybrid Mus musculus
44 RBM7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NOL11  
Affinity Capture-MS Homo sapiens
46 GOLGA2 2801
Two-hybrid Homo sapiens
47 DHRS2 10202
Two-hybrid Homo sapiens
48 CIRH1A 84916
Affinity Capture-MS Homo sapiens
49 TPI1 7167
Co-fractionation Homo sapiens
50 RPL36 25873
Affinity Capture-MS Homo sapiens
51 WDR43 23160
Affinity Capture-MS Homo sapiens
52 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SRPK3  
Affinity Capture-MS Homo sapiens
54 Exosc1  
Affinity Capture-MS Mus musculus
55 GNL3L 54552
Affinity Capture-MS Homo sapiens
56 MRPL41 64975
Affinity Capture-MS Homo sapiens
57 Nufip2  
Two-hybrid Mus musculus
58 UTP15 84135
Affinity Capture-MS Homo sapiens
59 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 BIRC2  
Two-hybrid Homo sapiens
61 SFPQ 6421
Two-hybrid Homo sapiens
62 PSMA3 5684
Co-fractionation Homo sapiens
63 HBS1L 10767
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 LRP4 4038
Affinity Capture-MS Homo sapiens
65 DOCK8 81704
Two-hybrid Homo sapiens
66 XPO1 7514
Affinity Capture-MS Homo sapiens
67 IKZF3  
Two-hybrid Homo sapiens
68 Rbm14  
Two-hybrid Mus musculus
69 CUL3 8452
Affinity Capture-MS Homo sapiens
70 PSMA2 5683
Co-fractionation Homo sapiens
71 EGFR 1956
Negative Genetic Homo sapiens
72 PAPD5 64282
Affinity Capture-MS Homo sapiens
73 ZNF558  
Two-hybrid Homo sapiens
74 PSPC1 55269
Affinity Capture-MS Homo sapiens
75 RPA3 6119
Proximity Label-MS Homo sapiens
76 LSM3 27258
Two-hybrid Homo sapiens
77 EXOSC3 51010
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 WDR75  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 SKIV2L2 23517
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 DDRGK1 65992
Affinity Capture-MS Homo sapiens
81 Pef1  
Two-hybrid Mus musculus
82 TFIP11  
Two-hybrid Homo sapiens
83 SCLY  
Affinity Capture-MS Homo sapiens
84 PWP2 5822
Affinity Capture-MS Homo sapiens
85 HUWE1 10075
Affinity Capture-MS Homo sapiens
86 RPS16 6217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 PA2G4 5036
Two-hybrid Homo sapiens
88 EXOSC10 5394
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 GIGYF2 26058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TRIM54  
Two-hybrid Homo sapiens
91 NMI  
Two-hybrid Homo sapiens
92 POLR2L 5441
Two-hybrid Homo sapiens
93 TNFAIP1 7126
Two-hybrid Homo sapiens
94 OSTM1 28962
Affinity Capture-Western Homo sapiens
95 THOP1 7064
Affinity Capture-MS Homo sapiens
96 TBL3 10607
Affinity Capture-MS Homo sapiens
97 YTHDF3  
Two-hybrid Homo sapiens
98 PSMA1 5682
Co-fractionation Homo sapiens
99 UFL1 23376
Affinity Capture-MS Homo sapiens
100 PSMA5 5686
Co-fractionation Homo sapiens
101 C16orf72 29035
Affinity Capture-MS Homo sapiens
102 SH3GLB1 51100
Two-hybrid Homo sapiens
103 CDK9 1025
Affinity Capture-MS Homo sapiens
104 TCAF1 9747
Affinity Capture-MS Homo sapiens
105 RPS7 6201
Affinity Capture-MS Homo sapiens
106 TJP2 9414
Affinity Capture-MS Homo sapiens
107 KRT13 3860
Two-hybrid Homo sapiens
108 KRAS 3845
Negative Genetic Homo sapiens
109 OGDH 4967
Affinity Capture-MS Homo sapiens
110 EXOSC1 51013
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
111 FGF1  
Affinity Capture-MS Homo sapiens
112 EXOSC8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 SH3GLB2 56904
Two-hybrid Homo sapiens
View the network image/svg+xml



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