Gene description for Nudt21
Gene name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Gene symbol Nudt21
Other names/aliases 25kDa
3110048P04Rik
5730530J16Rik
AU014860
AW549947
Cpsf5
Species Mus musculus
 Database cross references - Nudt21
ExoCarta ExoCarta_68219
Vesiclepedia VP_68219
Entrez Gene 68219
UniProt Q9CQF3  
 Nudt21 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nudt21
Molecular Function
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    protein binding GO:0005515 IPI
    mRNA 3'-UTR AU-rich region binding GO:0035925 IBA
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISO
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISS
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
Biological Process
    mRNA processing GO:0006397 IBA
    mRNA processing GO:0006397 ISO
    mRNA processing GO:0006397 ISS
    post-transcriptional regulation of gene expression GO:0010608 IMP
    cell differentiation GO:0030154 IEA
    mRNA 3'-end processing GO:0031124 ISO
    mRNA 3'-end processing GO:0031124 ISS
    protein tetramerization GO:0051262 ISO
    protein heterotetramerization GO:0051290 ISO
    protein heterotetramerization GO:0051290 ISS
    mRNA alternative polyadenylation GO:0110104 ISO
    mRNA alternative polyadenylation GO:0110104 ISS
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 ISO
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 ISS
    positive regulation of stem cell differentiation GO:2000738 IMP
    positive regulation of pro-B cell differentiation GO:2000975 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 ISO
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IEA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 ISO
    mRNA cleavage factor complex GO:0005849 IBA
    mRNA cleavage factor complex GO:0005849 ISO
    mRNA cleavage factor complex GO:0005849 ISS
    nuclear body GO:0016604 ISO
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
    paraspeckles GO:0042382 ISO
    paraspeckles GO:0042382 ISS
 Experiment description of studies that identified Nudt21 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nudt21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIP7 51388
Two-hybrid Homo sapiens
2 Mrpl13  
Two-hybrid Mus musculus
3 YTHDF3  
Two-hybrid Homo sapiens
4 EXOSC7 23016
Two-hybrid Homo sapiens
5 RBM42  
Two-hybrid Homo sapiens
6 DGCR14  
Two-hybrid Homo sapiens
7 Cnn3 71994
Two-hybrid Mus musculus
8 BCAS2 10286
Two-hybrid Homo sapiens
9 MAZ 4150
Two-hybrid Homo sapiens
10 QARS 5859
Two-hybrid Homo sapiens
11 Fndc5  
Affinity Capture-MS Mus musculus
12 TRNAU1AP  
Two-hybrid Homo sapiens
13 DENND2C  
Two-hybrid Homo sapiens
14 DMRTB1  
Two-hybrid Homo sapiens
15 YTHDF1 54915
Two-hybrid Homo sapiens
16 Cstf2t  
Two-hybrid Mus musculus
17 SLU7  
Two-hybrid Homo sapiens
18 NSUN2 54888
Two-hybrid Homo sapiens
19 LARP1B 55132
Two-hybrid Homo sapiens
20 Kctd13  
Affinity Capture-MS Mus musculus
21 TEX10 54881
Two-hybrid Homo sapiens
22 Klc4  
Two-hybrid Mus musculus
23 CPSF4  
Two-hybrid Homo sapiens
24 LENG1  
Two-hybrid Homo sapiens
25 CTBP1 1487
Two-hybrid Homo sapiens
26 EIF4A3 9775
Two-hybrid Homo sapiens
27 PQBP1  
Two-hybrid Homo sapiens
28 UBL5 59286
Two-hybrid Homo sapiens
29 RBM7  
Two-hybrid Homo sapiens
30 SUGP2  
Two-hybrid Homo sapiens
31 LSM5 23658
Two-hybrid Homo sapiens
32 NXF1 10482
Two-hybrid Homo sapiens
33 Mettl21e  
Affinity Capture-MS Mus musculus
34 Cpsf6  
Two-hybrid Mus musculus
35 Tmem173  
Proximity Label-MS Mus musculus
36 Usp48  
Affinity Capture-MS Mus musculus
37 RPL10A 4736
Two-hybrid Homo sapiens
38 MREG  
Two-hybrid Homo sapiens
39 EXOSC4 54512
Two-hybrid Homo sapiens
40 CXorf56  
Two-hybrid Homo sapiens
41 BAG4  
Two-hybrid Homo sapiens
42 EXOSC5 56915
Two-hybrid Homo sapiens
43 Grb2 14784
Two-hybrid Mus musculus
44 PRC1 9055
Two-hybrid Homo sapiens
45 CISD2 493856
Two-hybrid Homo sapiens
46 CPSF7 79869
Two-hybrid Homo sapiens
47 SUV39H1  
Two-hybrid Homo sapiens
48 Nono 53610
Two-hybrid Mus musculus
49 MAGOH 4116
Two-hybrid Homo sapiens
50 RPL7 6129
Two-hybrid Homo sapiens
51 SNRPA1 6627
Two-hybrid Homo sapiens
52 Pspc1  
Two-hybrid Mus musculus
53 MRPL28 10573
Two-hybrid Homo sapiens
54 TXNL4A 10907
Two-hybrid Homo sapiens
55 EIF3E 3646
Two-hybrid Homo sapiens
56 Cfd  
Affinity Capture-MS Mus musculus
57 ZNF830  
Two-hybrid Homo sapiens
58 ERAL1  
Two-hybrid Homo sapiens
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