Gene description for CTBP1
Gene name C-terminal binding protein 1
Gene symbol CTBP1
Other names/aliases BARS
Species Homo sapiens
 Database cross references - CTBP1
ExoCarta ExoCarta_1487
Entrez Gene 1487
HGNC 2494
MIM 602618
UniProt Q13363  
 CTBP1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CTBP1
Molecular Function
    transcription factor binding GO:0008134 IPI
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    repressing transcription factor binding GO:0070491 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    protein homodimerization activity GO:0042803 IEA
    RNA polymerase II transcription corepressor activity GO:0001106 IDA
    protein domain specific binding GO:0019904 IPI
    protein C-terminus binding GO:0008022 TAS
    NAD binding GO:0051287 ISS
    protein binding GO:0005515 IPI
Biological Process
    protein phosphorylation GO:0006468 TAS
    viral genome replication GO:0019079 TAS
    Golgi organization GO:0007030 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of histone deacetylation GO:0031065 IMP
    negative regulation of histone H4 acetylation GO:0090241 IMP
    oxidation-reduction process GO:0055114 IEA
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    negative regulation of transcription from RNA polymerase II promoter by histone modification GO:1903758 IMP
    negative regulation of histone acetylation GO:0035067 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    regulation of cell cycle GO:0051726 IMP
    negative regulation of cell proliferation GO:0008285 TAS
    white fat cell differentiation GO:0050872 ISS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    transcription factor complex GO:0005667 IEA
    nucleus GO:0005634 ISS
    cytosol GO:0005829 IEA
    transcriptional repressor complex GO:0017053 ISS
 Experiment description of studies that identified CTBP1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX4  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 Nrip1  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
3 HDAC4  
Reconstituted Complex Homo sapiens
4 FOXP2 93986
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 ZNFN1A1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 GEMIN2 8487
Affinity Capture-Western Homo sapiens
7 SIN3A  
Invivo Homo sapiens
8 FOXP1 27086
Affinity Capture-Western Homo sapiens
9 CTBP2 1488
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 ZEB1  
Invivo Homo sapiens
11 RB1 5925
Affinity Capture-MS Homo sapiens
12 MLL  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 TGIF  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 BRCA1 672
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
15 PCAF  
Biochemical Activity Homo sapiens
16 ZNFN1A4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 HDAC1 3065
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 GTF2B 2959
Reconstituted Complex Homo sapiens
19 ELK3  
Affinity Capture-MS Homo sapiens
20 ZFPM2  
Two-hybrid Homo sapiens
21 TBP  
Reconstituted Complex Homo sapiens
22 HTT 3064
Invivo Homo sapiens
23 HDAC3 8841
Reconstituted Complex Homo sapiens
24 HDAC2 3066
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
25 NRIP1 8204
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
26 ACTL6B  
Invivo Homo sapiens
Two-hybrid Homo sapiens
27 SMAD6  
Affinity Capture-Western Homo sapiens
28 MECOM 2122
Phenotypic Enhancement Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 RBBP8  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 Pc1  
Two-hybrid 8355
31 HDAC5 10014
Reconstituted Complex Homo sapiens
32 MDM2  
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 NOS1  
Invivo Homo sapiens
Invitro Homo sapiens
34 HDAC9  
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
35 PNN 5411
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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