Gene description for CDC23
Gene name cell division cycle 23
Gene symbol CDC23
Other names/aliases ANAPC8
APC8
CUT23
Species Homo sapiens
 Database cross references - CDC23
ExoCarta ExoCarta_8697
Entrez Gene 8697
HGNC 1724
MIM 603462
UniProt Q9UJX2  
 CDC23 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for CDC23
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 TAS
Biological Process
    regulation of exit from mitosis GO:0007096 TAS
    protein K11-linked ubiquitination GO:0070979 IDA
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    mitotic spindle assembly checkpoint GO:0007094 TAS
    cell division GO:0051301 IEA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    mitotic metaphase plate congression GO:0007080 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    mitotic nuclear division GO:0007067 IDA
    mitotic cell cycle GO:0000278 TAS
    regulation of mitotic metaphase/anaphase transition GO:0030071 IDA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 TAS
Subcellular Localization
    intracellular GO:0005622 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    anaphase-promoting complex GO:0005680 IDA
 Experiment description of studies that identified CDC23 in exosomes
1
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC4  
Affinity Capture-Western Homo sapiens
2 APC2  
Affinity Capture-Western Homo sapiens
3 TSC22D4  
Two-hybrid Homo sapiens
4 ANAPC5 51433
Invivo Homo sapiens
5 CDH1 999
Reconstituted Complex Homo sapiens
6 ANAPC1 64682
Co-purification Homo sapiens
7 RAD54B  
Two-hybrid Homo sapiens
8 RBPMS  
Two-hybrid Homo sapiens
9 CDC27 996
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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